Preparation method of corn stigma polysaccharide and application of corn stigma polysaccharide in hypoglycemic products

Abstract

The invention provides a preparation method of corn stigma polysaccharide and application of the corn stigma polysaccharide in a hypoglycemic product, and relates to the technical field of extraction.The preparation method specifically comprises the steps that 1, pretreatment is conducted, specifically, corn stigma is cleaned, dried, smashed and then sieved, corn stigma powder is obtained, the corn stigma powder is soaked with ethyl alcohol, impurities are removed, and after the operation is repeated for three times, degreased corn stigma powder is obtained; (2) extraction: adding water and compound protease into the degreased corn stigma powder, soaking, carrying out primary heating extraction, cooling, then adding bromelain and the solution A, soaking, carrying out secondary heating extraction to obtain a leaching solution, and removing precipitates after enzyme deactivation and centrifugation to obtain a corn stigma polysaccharide clear solution; (3) impurity removal: dialyzing the corn stigma polysaccharide clear liquid to obtain dialyzed internal liquid; and (4) post-treatment: washing and drying the dialysis internal liquid to obtain the corn stigma polysaccharide. High-yield and high-purity corn stigma polysaccharide extraction can be realized, and the corn stigma polysaccharide can be applied to hypoglycemic products.

Description

technical field [0001] The invention relates to the technical field of extraction, in particular to a preparation method of corn silk polysaccharide and its application in hypoglycemic products. Background technique [0002] Corn silk is the style and stigma of the gramineous plant maize. It has the functions of diuresis and swelling, clearing the liver and gallbladder, and has a wide range of sources, low price, and easy collection. It has a relatively broad research prospect. Corn silk polysaccharide is one of the main active components of corn silk, accounting for about 4.87% of the dry weight of corn silk, and the specific types include glucose, arabinose, galactose and mannose. Literature: Zhang Jiajia, He Zhitao, Wang Xinru, et al. Research progress on the pharmacological effects of corn silk polysaccharides [J]. Journal of Jilin University of Medicine, 2021, 42(1): 3. et al. recorded the effects of corn silk polysaccharides in anti-tumor, hypoglycemic, regulating The...

AI Technical Summary

Problems solved by technology

The preparation method of the invention includes degreasing by soaking in ethanol and deproteinizing by trichloroacetic acid method, which needs to consume a large amount of solvent and will cause waste water pollution in certain cases.

[0005] Based on the above problems, the patent CN200910067588.1 discloses the application of corn silk polysaccharide in the preparation of drugs for the treatment of hyperlipidemia. The preparation of corn polysaccharides has been realized, and the prepared corn polysaccharides have better hypoglycemic and blood lipid-lowering effects. This invention makes full use of the effect of protease, is simple to operate and has little effect on the activity of polysaccharides, and is efficient and safe. However, the invention records Overall technical complexity and high solvent consumption

[0006] In view of the low extraction purity of corn silk polysaccharides, large solvent consumption and serious pollution in the prior art, it is necessary to find a preparation method of corn silk polysaccharides with high extraction purity, low solvent consumption and environmental protection

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