Anti-adhesion tendon material and preparation method thereof

Abstract

The invention discloses an anti-adhesion tendon material and a preparation method thereof. The tendon material is composed of regulatory T lymphocyte exosomes and tendons, and is prepared by compounding the T lymphocyte exosomes and the tendons and then freeze-drying the T lymphocyte exosomes and the tendons. The tendon tissue of the prepared composite Treg cell exosome is exposed in body fluid, nutrient substances can be fully obtained, the growth of implanted tendon cells into the tendon tissue can be accelerated, the regeneration and reconstruction time of the tendon tissue can be shortened, the Treg cells can be cultured on a large scale, the Treg cell exosome is easy to obtain, and after the Treg cell exosome and the tendon material are freeze-dried, the preparation process is simple, and the preparation cost is low. The tendon material can be stored for a long time under the refrigeration condition or the room temperature condition, the shelf life is long, and the prepared tendon material is convenient to use and does not need physical isolation.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering regenerative medical products, in particular to an anti-adhesion tendon material and a preparation method thereof. Background technique [0002] Tendon injuries are a common sports injury that usually requires surgical repair or suturing. Due to the poor blood supply of the tendon, it is difficult for the tendon to heal itself after injury. About 40% of the patients will experience different degrees of tendon adhesion after surgery, which seriously affects the functional recovery of the patients. Current research believes that tendon adhesion is a kind of aseptic fibroproliferative inflammatory reaction of the body. The body releases vasoactive substances and cytokines such as histamine and bradykinin under the stimulation of various physical, chemical and biological factors, which makes the local Increased capillary permeability, leading to congestion, edema, and the formation of infl...

AI Technical Summary

Problems solved by technology

[0003] At present, physical isolation is one of the most commonly used clinical methods to reduce tendon adhesions. The most common ones on the market are silica gel membrane, hyaluronic acid membrane, and chitin membrane , polylactic acid membrane, biological amniotic membrane, autologous or allogeneic fascia, etc. (Zhou Xiujun. Evaluation of different biomaterials for preventing adhesion after tendon injury[J]. Chinese Tissue Engineering Research and Clinical Rehabilitation, 2010, 14(21): 3915-3918 Kang Dong. The anti-adhesion effect of absorbable biomaterials in tendon injury reconstruction [J]. Chinese Tissue Engineering Research, 2016, 20(21): 3185-3192.), as disclosed in patent 202010574129.9 is chitosan modified Collagen film, patent 202110906851.2 discloses that fresh amniotic membrane is loaded with the drug ecoxib, and patent 202110264223.9 is a layered composite film of polylactic acid and type I collagen in a certain proportion. These film products have a certain effect of preventing tendon adhesion. However, the adhesion between the membrane and the body is poor, sutures are often required during surgery, and the fixing and wrapping operation is cumbersome; at the same time, these membranes mainly isolate the fibrin, multinucleated cells, and inflammatory cells exuded around the tendon from the tendon through the physical barrier function, hindering It prevents the fibroblasts secreting collagen fibers from the tendon and plays an anti-adhesion role. In addition, physical isolation is not conducive to the tendon tissue to obtain nutrients and prolongs the regeneration and reconstruction process of the tendon tissue.

[0004] In addition, some researchers have used mesenchymal stem cell exosomes (Yao Zhixiao. The effect of human umbilical cord mesenchymal stem cell exosomes on tendon repair and related Mechanism research. Shanghai Jiao Tong University doctoral dissertation, June 2020.) or macrophage exosomes (Cui Haomin. Mechanism of macrophage exosome mi RNA-mediated tendon adhesion. Shanghai Jiao Tong University doctoral dissertation, 2019 In May 2008.) carried out the mechanism study of tendon anti-adhesion, but it is well known that umbilical cord mesenchymal stem cells often serve as the regulator of the local tissue immune microenvironment, and do not have the targeting of immune regulation; and so far, giant The role of phagocytes in the formation of tendon adhesions remains a controversial topic, with some studies suggesting that macrophage deficiency can significantly improve tissue healing and reduce peritendinous adhesion formation; some studies on macrophages and organ fibrosis Studies have also shown that the absence of macrophages can significantly improve organ healing and effectively prevent the development of fibrosis, further verifying that macrophages are an unfavorable factor in the healing of various tissues. Therefore, the use of macrophage exosomes to intervene Tendon adhesions may not be a better option

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