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Grease-rich streptozotoci and culture application thereof

A technology of chain algae and oil, applied in the fields of biotechnology and bioenergy, can solve problems such as unsatisfactory effects

Active Publication Date: 2022-05-13
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The microalgae disclosed in the above-mentioned patents are aimed at high temperature resistance, tolerance to SO 2 The microalgae bred with the same performance are not ideal when used in other aspects, so it is necessary to breed microalgae with more other functions, or to breed microalgae with multiple functions to make it more suitable for industrial applications

Method used

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  • Grease-rich streptozotoci and culture application thereof
  • Grease-rich streptozotoci and culture application thereof
  • Grease-rich streptozotoci and culture application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Separation and domestication screening to obtain Chainsella HCS-BY1

[0027] (1) Obtaining the starting algae strain: In October 2016, 150mL of water samples were retrieved from the Hunchun River in Hunchun City, Jilin Province, filtered with gauze to remove large impurities, and 50ml of the filtered water samples were inoculated into 200mL of BG11 The enrichment culture was carried out in the culture medium, the light intensity of the culture was 5000Lux, the temperature was 25°C, the light cycle was 24h, and the light-dark time ratio was 14:10. After about half a month of culture, the culture medium turned green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000Lux and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker ...

Embodiment 2

[0032] Example 2 Identification of algae strains

[0033] The DNA of HCS-BY1 algae cells was extracted by CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Desmodesmus abundans It has the greatest similarity, its BLASTn value is 2517, and its Max index value is 98.9%, so it can be determined that HCS-BY1 is a catenia ( Desmodesmus abundans ).

Embodiment 3

[0034] The application of embodiment 3 S. catenella HCS-BY1

[0035] The algae liquid of HCS-BY1 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formula of BG11 medium is shown in Table 1 and Table 2. The cultivation was carried out in a photobioreactor. The OD of the culture medium after inoculation was 690 is 0.22. CO was introduced from the bottom of the reactor 2 Flue gas with a content of 40v%, of which the NO content is 0.07v%, SO 2 The content is 0.05v%. During the cultivation process, the light intensity was 8000Lux, the cultivation temperature was 28°C, the pH value was controlled at 7-8, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 8 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder was measured to calculate the biomass production, and th...

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Abstract

The invention relates to a strain of grease-rich Streptomyces sp. And a culture application thereof, the strain is Streptomyces sp. HSC-BY1, the classification name of the strain is Streptomyces sp. HSC-BY1, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 12, 2020, and the preservation number is CGMCC No.19982. The invention also relates to a method for preparing the grease-rich Streptomyces sp. HSC-BY1. The grease-rich Streptomyces sp. HSC-BY1 can be used for preparing the grease-rich Streptomyces sp. HSC-BY1. The streptozotocaceae provided by the invention is high in nitrogen fixation efficiency, and the obtained biomass is rich in grease; particularly, the catalyst has the function of resisting NOx and SOx at the same time.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to an oil-rich algae and its cultivation application. Background technique [0002] Since the beginning of the 21st century, there is a close relationship between economic development and energy consumption. Energy security is a strategic issue for all countries. Finding a clean, economical, efficient and renewable new energy has become an urgent problem to be solved. Among them, bioenergy has always been an important reserve technology direction, and various countries have maintained interest in it. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/6463B01D53/85B01D53/62B01D53/50B01D53/56C12R1/89
CPCC12N1/12C12P7/6463B01D53/85B01D53/62B01D53/508B01D53/565B01D2258/0283B01D2257/504B01D2257/404B01D2257/302Y02A50/20
Inventor 师文静樊亚超张霖李晓姝廖莎王鹏翔朱化雷
Owner CHINA PETROLEUM & CHEM CORP
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