Nucleic acid functionalized MOF (Metal Organic Framework) material as well as preparation and application thereof
A functionalized, nucleic acid technology, applied in the field of nanomaterials and biosensing, to achieve the effect of enhanced fluorescence signal, excellent stimuli-responsive release performance, and broad application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] Taking the determination of ATP as an example to prepare nucleic acid functionalized MOF:
[0037] 1) 488mg ZrCl 4 and 218mg of 2-amino-4,4'-biphenyldicarboxylic acid were added to 30mL of DMF solution, ultrasonically dissolved for 15min to obtain a mixed solution, and then 3.6mL of acetic acid was added to the mixed solution and ultrasonically dissolved for 5min. Then, the reaction mixture was hydrothermally reacted at 100°C for 24 hours to generate UiO-67-NH 2 ; The resulting MOF product (UiO-67-NH 2 ) were washed 3 times with DMF and absolute ethanol respectively, and then vacuum-dried at 50°C (see figure 2 A).
[0038] 2) Mix 250 μL complementary strand DNA (100 μM, 5’COOH-GCAACAACGTACCCAATG3’) with 125 μL EDC (5 mgmL -1 ), 125μL NHS (5mg mL -1 ) stirred and reacted for 20min, then 5mg of the above-mentioned obtained UiO-67-NH 2 Added to the reaction mixture and stirred for 15 hours to obtain modified UiO-67, then washed the modified UiO-67 with PBS (10 mM, p...
Embodiment 2
[0042] Taking the determination of ATP as an example to prepare nucleic acid functionalized MOF:
[0043] 1) 488mg ZrCl 4 and 188 mg of terephthalic acid were added to 30 mL of DMF solution, ultrasonically dissolved for 15 minutes to obtain a mixed solution, and then 3.6 mL of acetic acid was added to the mixed solution for ultrasonically dissolved for 5 minutes. Then, the reaction mixture was hydrothermally reacted at 100°C for 24 hours to generate UiO-66-NH 2 . The resulting MOF product (UiO-66-NH 2 ) were washed 3 times with DMF and absolute ethanol respectively, and then vacuum-dried at 50°C (see image 3 A).
[0044] 2) Mix 250 μL complementary strand DNA (100 μM, 5’COOH-GCAACAACGTACCCAATG3’) with 125 μL EDC (5 mgmL -1 ), 125μL NHS (5mg mL -1 ) stirred and reacted for 20min, then 5mg of the above-mentioned obtained UiO-66-NH 2 Added to the reaction mixture and stirred for 15 hours to obtain modified UiO-66, then washed the modified UiO-66 with PBS (10 mM, pH=7.4), ...
Embodiment 3
[0048] Utilize above-mentioned embodiment to obtain material to carry out the detection of ATP:
[0049] Add 40 μL of the above to 300 μL PBS (10 mM, pH=7.4) to obtain Rho 6G@UiO-66 (250 μgmL -1 ) or Rho 6G@UiO-67 (250μg mL -1 ). Then add 20 μL exonuclease I (800 U mL -1 ) (purchased from Thermo Fisher Scientific) and 40 μL of ATP solution (10 nM). Take 100 μL of reaction solution at different times, centrifuge at 10,000 rpm for 3 minutes, measure the fluorescence intensity of the supernatant at an excitation wavelength of 525 nm and an emission wavelength of 552 nm; record the dye release of Rho 6G@UiO-66 and Rho 6G@UiO-67 obtained from the above examples Curve, at the same time with no exonuclease I, only added ATP sample as a control, no exonuclease I and no added ATP sample as a blank (see Figure 4 ).
[0050] Such as Figure 4 shown. The curve shows that within 30 min, the fluorescence of the sample without adding ATP changes very little. Once the target is prese...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap