Molecular marker primer of major gene locus TaIGC-7B of wheat glutelin macromer content and application of molecular marker primer

Abstract

The invention discloses a molecular marker primer of a major gene locus TaIGC-7B of the large polymer content of wheat glutelin and application of the molecular marker primer. Specifically, the invention discloses an application of an Indel molecular marker related to the glutelin large polymer content and / or a substance for detecting the molecular marker in identification or auxiliary identification of the wheat glutelin large polymer content. The invention designs a primer of the molecular marker and provides a method for identifying the large polymer content of wheat glutelin. The molecular marker as well as the primer and the method thereof have the characteristics of high specificity and single amplified DNA fragment, are convenient to detect, can be detected in each tissue and development stage of wheat, and can be used for screening in the seedling stage of the wheat, so that the breeding cost is saved, the selection efficiency of variety quality improvement is improved, and the selective breeding process of the wheat is accelerated. The molecular marker is a co-dominant marker developed according to Indel, can reduce linkage burdens during molecular marker-assisted selection, and has great application value.

Description

technical field [0001] The invention belongs to the technical field of molecular marker-assisted breeding, and in particular relates to a molecular marker primer of TaIGC-7B, a major gene locus of wheat glutenin macromer content, and its application. Background technique [0002] Wheat (Triticum aestivum L.) is one of the most important food crops in my country, and its production and consumption rank first in the world. Wheat flour has special noodle-making properties after adding water. The dough is both elastic and extensible, and can be used to process various foods, such as bread, noodles, steamed bread, biscuits and pastries. The current research has shown that the protein content and composition in wheat grains have a very important influence on the quality. Wheat grain protein mainly includes two parts: albumin and globulin, which are related to metabolism, and gluten protein, which is closely related to quality. In recent years, with the development of the economy...

AI Technical Summary

Problems solved by technology

Although it is known that glutenin and gliadin are the main components of gluten complex, the genes and molecular mechanisms that promote the formation and function of gluten complex are still unclear, which is very important for effectively regulating gluten complex and improving grain processing quality Adverse

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