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ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide

A technology of targeting peptides and mixtures, applied in the field of biomedicine, can solve the problems of large molecular weight, high cost and unstable structure of monoclonal antibodies, and achieve the effect of inhibiting the infection of new coronavirus

Active Publication Date: 2022-04-19
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the molecular weight of monoclonal antibodies is large, the blocking efficiency is low, the structure is unstable, and it is easy to lose activity at room temperature.
Therefore, the production, transportation and storage of monoclonal antibodies all require low temperature conditions, and the cost is high

Method used

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  • ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide
  • ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide
  • ACE2 targeting peptide in targeted combination with ACE2 protein and application of ACE2 targeting peptide

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Embodiment 1

[0041] Using the phage 12 peptide library constructed by the phage display technology, four rounds of screening were conducted for ACE2 protein-specific binding positive polypeptides. Due to the large abundance of the phage 12 peptide library, 10 μL of the phage 12 peptide library was taken for screening during the screening process, and the experiment successfully obtained the polypeptide sequence that can efficiently target the ACE2 protein through the following operation steps.

[0042] 1.1. Resuscitation and culture of host bacteria E.coil.ER2738

[0043] Prepare LB solid-state culture plates and place in a 37 °C incubator for 1 h. Using a bacterium pick stick, dip the melted E.coil.ER2738 bacterial solution, apply a "Z" shape to the surface of the LB plate, and place in a 37 °C incubator for 12 hours. Add 5 mL of liquid LB medium containing tetracycline to the shaker tube, pick out the monoclonal colonies in the plate using a sterile tip and place the tip in the medium. Place...

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Abstract

The invention discloses an ACE2 targeting peptide in targeting combination with ACE2 protein and application of the ACE2 targeting peptide. The ACE2 targeting peptide is a polypeptide, and the amino acid sequences of the ACE2 targeting peptide are respectively as follows: FPHWHGNHKHNR, GFKSHFHHNHMR, AHYHRHPPHLNR, LSPHFHHHRGAK, METRPVAPHEFR, LHRPHFHAHNNS, GHQGHWYGMFRA, YPWHHRHHMPVHP, SHKHHWPYHAH and FHGHGSHNKHR; the bioactive substance is formed by forming ACE2 targeting peptide on the surface of a mixture of microorganisms and polymers. The ACE2 protein can be efficiently targeted, the combination of the novel coronavirus spike protein and the ACE2 protein is blocked, and the effect of inhibiting novel coronavirus infection is further achieved.

Description

Technical field [0001] The present invention belongs to a polypeptide in the biomedical field and its uses, in particular to a polypeptide capable of targeting binding angiotensin-converting enzyme 2 (ACE2) and its uses. Background [0002] Angiotensin-converting enzyme 2 (ACE2) is a protein present on the surface of a variety of cells. The spike protein on the surface of the new coronavirus can target the recognition of the ACE2 protein, and through the interaction of the spike protein with ACE2, the new coronavirus can enter the human cells, causing pneumonia. [0003] Usually, researchers mainly use monoclonal antibodies to block the binding of the ACE2 protein to the new coronavirus spike protein. However, monoclonal antibodies have large molecular weight, low blocking efficiency, unstable structure, and are prone to inactivity at room temperature. Therefore, the production, transportation and preservation of monoclonal antibodies require low temperature conditions and high ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/14
CPCC07K7/08A61P31/14A61K38/00Y02A50/30
Inventor 毛传斌鲍青杨涛杨明英
Owner ZHEJIANG UNIV
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