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Electrochemiluminescence immunosensor for non-small cell lung cancer CYFRA21-1 detection and detection method

A technology of non-small cell lung cancer and luminescent immunity, which is applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of expensive equipment, cumbersome operation, and high cost, and achieve reduced experimental costs, rapid signal response, and less usage Effect

Active Publication Date: 2022-03-25
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sensitivity is high, there are disadvantages such as time-consuming, high cost, expensive equipment and cumbersome operation. Therefore, it is necessary to develop a simple, sensitive, fast and economical method for the detection of CYFRA21-1 in clinical samples.

Method used

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  • Electrochemiluminescence immunosensor for non-small cell lung cancer CYFRA21-1 detection and detection method
  • Electrochemiluminescence immunosensor for non-small cell lung cancer CYFRA21-1 detection and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Preparation of Composite Nanomaterials and Construction of Electrochemiluminescent Immunosensors

[0061] Follow the steps below (construct the schematic as figure 1 shown):

[0062] Step 1: ZIF-67 dispersion: 1.455 g Co(NO 3 ) 2 ·6H 2 O and 1.65 g of 2-methylimidazole were dissolved in 30 mL of methanol, respectively. The above solutions were mixed after sonication and stirred at room temperature for 3 hours. The mixture was centrifuged to obtain a brown powder, which was washed with methanol and water, and dried at 60°C for further use. Finally, the brown powder was dispersed in ultrapure water to obtain a ZIF-67 dispersion with a concentration of 1 mg / ml.

[0063] Step 2: La-MOF dispersion: add 2 mmol La(NO 3 )3 and 2-aminoterephthalic acid were slowly added to 30 mL DMF solution. After ultrasonic treatment to uniform dispersion, the mixture was stirred at room temperature for 10 min, then transferred to a stainless steel autoclave lined with polytetrafluoroe...

Embodiment 2

[0080] Detection of CYFRA21-1 in non-small cell lung cancer by electrochemiluminescence immunosensor

[0081] Using the electrochemiluminescence immunosensor constructed in Example 1 to detect CYFRA21-1, follow the steps below:

[0082] (1) Draw the standard curve:

[0083] Add 10 μL of the signal probe prepared in step 5 to the surface of the electrode in the immunosensor constructed in Example 1, and place the electrode in a solution containing 5 mM H 2 o 2 Characterized in 0.1 M PBS solution, and measured the current values ​​of different concentrations of CYFRA21-1. A standard curve was drawn according to the logarithmic value and ECL response signal of different concentrations of CYFRA21-1. The test results showed that the two had a good linear relationship in the concentration range of 100 fg / mL - 100 ng / mL, the linear correlation coefficient was 0.9980, and the detection limit was 85.20 fM, the results are detailed in figure 1 .

[0084] (2) Sensor stability detect...

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Abstract

The invention provides an electrochemical luminescence immunosensor for detecting non-small cell lung cancer CYFRA21-1, which is characterized in that firstly, an MXene-PEI-NiMn LDH novel nano composite material is prepared as a substrate material of the sensor, a La-MOF-ZIF-67 bimetal organic framework is used for carrying a luminophor Luminol, and the electrochemical luminescence immunosensor for detecting the CYFRA21-1 is constructed by utilizing a sandwich method. The substrate material MXene-PEI-NiMn LDH and the bimetal organic framework La-MOF at ZIF-67 are used as co-reaction promoters to synergistically catalyze hydroxyl radicals generated by H2O2, the sensor not only has a stronger signal amplification effect, but also has the advantages of wide linear range, strong specificity, short analysis time and the like, and a new method can be provided for diagnosis of non-small cell lung cancer. The detection method is simple to operate and high in practicability.

Description

technical field [0001] The invention belongs to the technical field of electrochemiluminescence immunosensors and nanocomposite materials, and relates to an electrochemiluminescence immunosensor and a detection method for detecting non-small cell lung cancer CYFRA21-1. Background technique [0002] Lung cancer is one of the most common malignancies in the world and has become the leading cause of cancer-related death worldwide. Lung cancer is divided into small cell lung cancer and non-small cell lung cancer, among which non-small cell lung cancer (NSCLC) accounts for about 85% of all lung cancers, and about 75% of NSCLC patients are already at an advanced stage when they are discovered. Existing diagnostic methods have the disadvantages of high price, complicated and time-consuming process, and great damage to the patient's body. Therefore, for non-small cell lung cancer, it is of great clinical significance to explore a low-cost, simple and fast disease diagnosis method w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/543G01N33/531G01N33/574
CPCG01N27/26G01N27/3278G01N27/308G01N33/54306G01N33/531G01N33/57423Y02A50/30
Inventor 母昭德郭蕾白丽娟
Owner CHONGQING MEDICAL UNIVERSITY
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