Method for reducing viscosity of thickened oil by using microbial conversion liquid

A technology for microbial transformation and viscosity of heavy oil, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of serious heat loss, decline in crude oil recovery, poor thermal energy utilization, etc., to promote stripping. , Amplify the effect of easy construction and viscosity reduction

Pending Publication Date: 2022-03-25
ZHEJIANG UNIV OF TECH
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AI Technical Summary

Problems solved by technology

Thermal viscosity reduction After multiple rounds of thermal recovery in the same oil reservoir, the thermal energy utilization rate is poor, the heat loss is serious, and the oil recovery rate inevitably declines
Blending with thinner for viscosity reduction cannot fundamentally solve the problem of viscosity reduction for heavy oil

Method used

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  • Method for reducing viscosity of thickened oil by using microbial conversion liquid
  • Method for reducing viscosity of thickened oil by using microbial conversion liquid
  • Method for reducing viscosity of thickened oil by using microbial conversion liquid

Examples

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Embodiment 1

[0024] The microbial transformation solution preparation method of Corynebacterium glutamicum comprises the following steps:

[0025] 1) Cultivate Corynebacterium glutamicum preserved at -80°C for 12 hours at 30°C and 120rpm / min in LB liquid medium at a 5% (v / v) inoculum size;

[0026] 2) According to the inoculum amount of 5% (v / v), inoculate the bacterium cultivated in 1) into the CGXⅡ liquid medium, and cultivate it at 30°C and 120rpm / min for 12h; Body, frozen in -20 ℃ refrigerator for 12h, then thawed at room temperature;

[0027] 3) Prepare transformation solution: glucose concentration 15g / L, L-leucine concentration 11.2g / L, pH=8.0, 20mM PBS buffer;

[0028] 4) Put the thawed bacteria in step 2) into the transformation solution of step 3) at a concentration of 20 g / L, and perform a transformation reaction at 30° C. for 10 h; after the reaction, centrifuge the transformation solution, and take the centrifuged supernatant, namely For microbial transformation fluid.

[0...

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Abstract

The invention discloses a method for reducing viscosity of thickened oil by using a microbial conversion liquid, which comprises the following steps of: preparing the microbial conversion liquid of corynebacterium glutamicum in pure water with the concentration of 0.1-1%, and injecting the conversion liquid into an oil reservoir or an oil-containing porous medium through a producing well to promote the stripping of the thickened oil and improve the rheological property of the crude oil, so that the viscosity of the thickened oil is reduced. The oil-phase flow resistance is reduced, the viscosity of the heavy oil is reduced, after the viscosity reduction treatment of the heavy oil in an oil reservoir or an oil-containing porous medium is completed, oil displacement can be carried out by adopting an existing conventional method, and the oil displacement difficulty is greatly reduced. The method for reducing the viscosity of the thickened oil by using the microbial conversion liquid has the advantages of low cost, environmental friendliness, simple process, easiness in amplified construction and capability of effectively reducing the viscosity of the thickened oil, and has a wide application prospect in the field of thickened oil recovery.

Description

technical field [0001] The invention belongs to the field of biology and petroleum exploitation, and in particular relates to a method for reducing the viscosity of heavy oil by utilizing microbial transformation liquid. Background technique [0002] Worldwide, heavy oil reserves are abundant, and they are playing an increasingly important role in the process of crude oil extraction. Because heavy oil is rich in high-viscosity substances such as asphaltene and colloid, it has high seepage resistance and poor fluidity, making it difficult to mine and transport. [0003] In the prior art, the methods of reducing the viscosity of heavy oil are usually through chemical viscosity reduction, thinning, thermal viscosity reduction and other methods. Among them, chemical viscosity reduction increases the difficulty of downstream refining due to the addition of chemical agents, and is not friendly to the environment. Thermal viscosity reduction After multiple rounds of thermal recov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E21B43/22C09K8/582C12N1/20C12R1/15
CPCE21B43/16C09K8/582C12N1/20
Inventor 贠军贤黄葆凌刘谭锦楼小玲张玮沈绍传
Owner ZHEJIANG UNIV OF TECH
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