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PH and photo-thermal dual-response drug-loaded nanoparticles, injectable collagen hydrogel system as well as preparation method and application of injectable collagen hydrogel system

A technology of collagen hydrogel and drug-loaded nanometers, which can be used in pharmaceutical formulations, nervous system diseases, medical preparations of non-active ingredients, etc., can solve the problems of insufficient drug release kinetics and functionality, and achieve long-term retention and Controlled release, scavenging free radicals, good photothermal responsiveness

Active Publication Date: 2022-03-15
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still shortcomings in the field of traditional metal-organic frameworks for drug loading, such as: (1) The toxicity evaluation of MOFs materials is still controversial, and chemical modification or compounding with other safer materials is often required to reduce their toxicity; (2) Drug release kinetics problems and insufficient functionality, for drug carriers used in spinal cord injury, in addition to high loading capacity and long-term retention in vivo, it also needs to have the ability to control release at a specific time

Method used

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  • PH and photo-thermal dual-response drug-loaded nanoparticles, injectable collagen hydrogel system as well as preparation method and application of injectable collagen hydrogel system
  • PH and photo-thermal dual-response drug-loaded nanoparticles, injectable collagen hydrogel system as well as preparation method and application of injectable collagen hydrogel system
  • PH and photo-thermal dual-response drug-loaded nanoparticles, injectable collagen hydrogel system as well as preparation method and application of injectable collagen hydrogel system

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0062] This preparation example prepares a kind of ZIF-8 of loading drug paclitaxel (PTX), and specific method is as follows:

[0063] ZIF-8 was dissolved in DMSO, ultrasonically dispersed, blended with PTX solution, the final concentration of ZIF-8 was 5 mg / mL, the final concentration of PTX was 500 μg / mL, and incubated at 25°C for 24h. The product was centrifuged at 12000 rpm for 15 min, and the precipitate was washed 3 times with PBS to obtain the drug-loaded ZIF-8, which was designated as ZIF-8 / PTX.

preparation example 2

[0065] This preparation example prepares a kind of ZIF-8 that the surface is coated with the load drug paclitaxel (PTX) of thermoresponsive phase change material, and specific method is as follows:

[0066] The synthesized ZIF-8 / PTX is dispersed in aqueous ethanol (ethanol volume fraction is 50%), then n-tetradecyl alcohol solution is mixed with it, ZIF-8 / PTX final concentration is 5mg / mL, n-tetradecyl alcohol final concentration 5mg / mL, stirred at 25°C for 3min. The product was centrifuged at 12000 rpm for 5 min, and the precipitate was washed 3 times with PBS to obtain a ZIF-8 loaded with drug paclitaxel (PTX) coated with a thermoresponsive phase change material on the surface, which was designated as PCM@ZIF-8 / PTX.

preparation example 3

[0068] This preparation example prepares a ZIF-8 loaded with drug paclitaxel (PTX) whose surface is coated with a heat-responsive phase-change material and a polyphenol compound, that is, a drug-loaded nanoparticle with a dual response to pH and light and heat involved in the present invention. The specific method as follows:

[0069] The synthesized PCM@ZIF-8 / PTX and tannic acid were mixed and dispersed in pure water, the final concentration of tannic acid in the mixed solution was 40 mg / mL, FeCl 3 The final concentration was 10mg / mL, the final concentration of PCM@ZIF-8 / PTX was 5mg / mL, and stirred at 25°C for 24h. The product was centrifuged at 12000rpm for 5min, and the precipitate was washed 3 times with PBS to obtain a ZIF-8 loaded with drug paclitaxel (PTX) coated with a thermoresponsive phase change material and a polyphenolic compound on the surface, which was designated as TA-PCM@ZIF-8 / PTX.

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Abstract

The invention relates to a pH and photo-thermal dual-response drug-loaded nanoparticle, an injectable collagen hydrogel system as well as a preparation method and application of the injectable collagen hydrogel system. The pH and photo-thermal dual-response drug-loaded nano particle comprises a ZIF-8 metal framework loaded with drug paclitaxel, a thermal response phase change material encapsulated on the surface of the ZIF-8 metal framework, and a polyphenol compound encapsulated on the surface of the thermal response phase change material. According to the drug-loaded nano particle, ZIF-8 is used as a carrier material to load a drug paclitaxel, high drug loading capacity and drug release of pH responsiveness at a spinal cord injury inflammation part are achieved, the thermal response phase change material endows the drug-loaded nano particle with a photo-thermal response function, the polyphenol compound improves the biocompatibility of the drug-loaded nano particle, and the drug-loaded nano particle has a good application prospect. The nano-particles have good light absorption effect in a near-infrared region and also have good photo-thermal responsiveness, and due to the existence of phenolic hydroxyl active groups, a physical or chemical stabilization effect can be generated between polyphenol and collagen, so that the nano-particles are endowed with the capability of combining with the collagen and promoting the cross-linking of the collagen.

Description

technical field [0001] The invention belongs to the technical field of nano-medicine delivery systems, and relates to a drug-loaded nanoparticle, an injectable collagen hydrogel system and its preparation method and application, in particular to a drug-loaded nanoparticle with dual response to pH and light and heat, and an injectable Collagen hydrogel system and its preparation method and application. Background technique [0002] In the past decade, the incidence of spinal cord injury repair has increased year by year. There are three key elements in the treatment of spinal cord injury repair, namely scaffolds, cells and drugs / factors. Paclitaxel PTX is a common anticancer drug. After dorsal spinal cord injury, paclitaxel can significantly reduce the scar formation at the injury site and the secretion and deposition of extracellular matrix ECM in the spinal cord contusion injury model. Appropriate paclitaxel concentration can effectively promote axon elongation and neural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/58A61K9/56A61K9/06A61K31/337A61K47/69A61K47/42A61K47/32A61P25/00
CPCA61K9/5015A61K9/5031A61K9/5073A61K9/06A61K9/0024A61K31/337A61K47/6949A61K47/42A61K47/32A61P25/00
Inventor 陈艳艳孙欣月戴建武
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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