Preparation method and detection method of electrochemical sensor for analyzing tea polyphenol and components thereof

Abstract

The invention discloses a preparation method and a detection method of an electrochemical sensor for analyzing tea polyphenol and components thereof. The preparation method comprises the following steps: S1, preparing a carboxylated multi-walled carbon nanotube / sodium lignosulfonate composite material; s2, preparing a carboxylated multi-walled carbon nanotube / sodium lignin sulfonate modified screen-printed electrode; s3, preparing a nanogold / carboxylated multi-walled carbon nanotube / sodium lignin sulfonate modified working electrode; s4, placing the constructed sensor in standard solutions of epigallocatechin gallate or rutin with different concentrations, and drawing a working curve; s5, the constructed sensor is placed in a citric acid buffer solution for detection; s6, recording the oxidation peak current value in the step S5, and measuring the concentration of total catechin, rutin and tea polyphenol in the sample according to the working curve in the step S4; the sensor prepared by the invention can simultaneously measure the content of catechin compounds and rutin, does not need complex sample pretreatment during detection, and is simple to operate, short in detection time and low in cost.

Description

technical field [0001] The invention relates to a preparation method and a detection method of an electrochemical sensor for analyzing tea polyphenols and its components, and belongs to the field of electrochemical sensors. Background technique [0002] Tea has a long history as a beverage in my country. Tea polyphenols are important taste and functional substances in tea, accounting for about 14%-30% of the dry weight of tea. Intake of tea polyphenols is beneficial to human health, and has various health functions such as anti-oxidation, anti-cancer, lowering blood sugar and blood pressure, anti-virus and anti-aging. The content of tea polyphenols is an important factor to evaluate the quality of tea; at present, spectrophotometry is one of the commonly used methods, with gallic acid as the standard sample, it can quickly and easily measure the total polyphenol content in tea, but it cannot distinguish catechu amount of ketones and flavonoids. Liquid chromatography and m...

AI Technical Summary

Problems solved by technology

The content of tea polyphenols is an important factor to evaluate the quality of tea; at present, spectrophotometry is one of the commonly used methods, with gallic acid as the standard sample, it can quickly and easily measure the total polyphenol content in tea, but it cannot distinguish catechu Amount of Vegetables and Flavonoids

Liquid chromatography and mass spectrometry can accurately detect the types and contents of tea polyphenols, but the equipment is relatively expensive, and the pretreatment and detection processes are usually complicated and time-consuming

[0003] The electrochemical detection method is a detection method developed in recent years. In order to improve the selectivity and sensitivity of electrochemical detection, nanomaterials are used to increase the electrochemical performance of the sensor; carbon nanotubes have excellent electrochemical behavior and high chemical stability. , biocompatibility and high specific surface area carbon nanomaterials, but due to its large van der Waals force and hydrophobic interaction, the multi-walled carbon nanotubes are agglomerated, and it is difficult to obtain a stable and uniform suspension

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