Genetically engineered cell membrane bionic nano-microsphere with pancreatic cancer microenvironment targeting and method thereof

Abstract

The invention discloses a genetic engineering cell membrane bionic nano-microsphere with pancreatic cancer microenvironment targeting and a preparation method of the genetic engineering cell membrane bionic nano-microsphere. The tumor-associated macrophage targeting peptide is expressed on the surface of pancreatic cancer cells KPC in a lentivirus transfection mode, and a pancreatic cancer cell line overexpressed by the macrophage targeting peptide M2pep is constructed; a pancreatic cancer first-line chemotherapy drug gemcitabine is loaded in a polylactic acid-glycollic acid polymer by a multiple emulsion method, and polylactic acid-glycollic acid nanoparticles are formed by self-assembly; the cell membrane vesicles of a pancreatic cancer cell line are extracted by using a gradient centrifugation method, and the polylactic acid-glycollic acid nano-microspheres are entrapped to obtain the pancreatic cancer microenvironment enhanced targeting gene engineering cell membrane bionic nano-microspheres. The cell membrane bionic nano-microspheres can be enriched in pancreatic cancer tissues in a large amount, non-specific accumulation of other tissues in a body is reduced, a specific targeting effect on the pancreatic cancer tissues is achieved, and the cell membrane bionic nano-microspheres have the advantage of accurately delivering a chemotherapeutic drug gemcitabine to the pancreatic cancer tissues in a large amount.

Description

Technical field [0001] The present invention relates to a biomedical material, and more particularly to a gene engineered cell membrane bionic nano microsphere and preparation method thereof, which have a microenvironment targeted by pancreatic cancer, and is a preparation for bionic nanometers. Background technique [0002] Pancreatic cancer is a malignant tumor that occurs on the pancreas, accounting for about 1% -2% of the body malignant tumor, and has an upward trend in recent years. The site of tumor growth is most common in pancreatic heads, approximately 60%, followed by the cutting of the pancreatic tail, the least pancreatic cancer, only 5%. Male is more than women, with high age of 40-65 years old. The disease is the highest, and the number of deaths is ranked fourth. Due to its clinical manifestation, the pathogenesis is very difficult, and the vast majority of diagnosis is late. Based on nanometric chemotherapy in recent years, it is a hot direction in the field of na...

AI Technical Summary

Problems solved by technology

Chemotherapy currently has limited efficacy and severe side effects

Images