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Recombinant adenovirus for expressing African swine fever virus B602L-B646L protein and construction method thereof

A B602L-B646L, African swine fever virus technology, applied in the field of genetic engineering

Pending Publication Date: 2022-03-01
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no recombinant adenovirus against B602L-B646L and a feasible method for preparing the recombinant adenovirus

Method used

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  • Recombinant adenovirus for expressing African swine fever virus B602L-B646L protein and construction method thereof
  • Recombinant adenovirus for expressing African swine fever virus B602L-B646L protein and construction method thereof
  • Recombinant adenovirus for expressing African swine fever virus B602L-B646L protein and construction method thereof

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Embodiment Construction

[0025] Various exemplary embodiments of the present invention will now be described in detail. The detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features and embodiments of the present invention.

[0026] It should be understood that the terminology described in the present invention is only used to describe specific embodiments, and is not used to limit the present invention. In addition, regarding the numerical ranges in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range. ...

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Abstract

The invention discloses a recombinant adenovirus for expressing African swine fever virus B602L-B646L protein and a construction method thereof, and belongs to the technical field of genetic engineering. A recombinant adenovirus shuttle vector pENTRE-EGFP-TOPO is utilized, and a recombinant adenovirus vector pAD-CMV-EGFP-B602L-B646L is obtained through a series of intermediate processes; according to the present invention, the African swine fever virus B602L-B646L protein expression recombinant adenovirus is constructed, the African swine fever virus B602L-B646L protein expression recombinant adenovirus is linearized and transfected with AD293 cells, the recombinant virus is screened according to the cytopathic effect formed by adenovirus infection, the adenovirus packaging process is achieved, the recombinant adenovirus expressing the African swine fever virus B602L-B646L protein is obtained, and the foundation is laid for the construction of the recombinant adenovirus vaccine expressing the African swine fever virus

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a recombinant adenovirus expressing African swine fever virus B602L-B646L protein and a construction method. Background technique [0002] African swine fever (African swine fever, ASF) is an acute, severe, highly contagious infectious disease caused by African swine fever virus (ASFV) infection. Clinical manifestations include fever, cyanosis of the skin, obvious bleeding of lymph nodes, kidneys, and gastrointestinal mucosa. [0003] African swine fever virus is the only member of the African swine fever virus family and the African swine fever virus genus. It is also the only DNA arbovirus at present. It is generally believed that there is only one serotype. Internationally, according to a 478bp nucleic acid sequence at the end of the ASFV B646L gene, it will be ASFV is divided into 24 genotypes, most of which belong to genotype Ⅱ in my country. African swine fever...

Claims

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Application Information

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IPC IPC(8): C12N15/861C12N15/64C12N15/34A61K39/12A61P31/20
CPCC12N15/86C07K14/005A61K39/12A61P31/20C12N2710/10343C12N2800/107C12N2710/12022C12N2710/12034A61K2039/5256A61K2039/53A61K2039/552
Inventor 朱鸿飞贾红鑫婷郭晓宇袁维峰崔帅王洋
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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