Pig virus gene chip and detection method thereof

Abstract

The invention provides a pig virus gene chip and a detection method thereof. The gene chip comprises a probe fixedly arranged on a substrate carrier, wherein the probe is selected from the characteristic segments of the following viruses: a classical swine fever virus (CSFV), a porcine reproductive and respiratory comprehensive virus (PRRSV), a pseudorabies virus (PRV), a porcine circovirus (PCV) and a porcine parvovirus (PPV). The types of the viruses detected by the method are various and the viruses cover common porcine viruses substantially. Random primer polymerase chain reaction (PCR) and multiplex-PCR are adopted to mark, so that false positive which is easy to cause when a PCR result is detected by gel electrophoresis is avoided, and high-flux accurate detection with short time is realized. The substrate carrier is a glass sheet which is subjected to aldehyde treatment, so that the combination between the probe and a target is facilitated, and higher noise is not brought to detection.

Description

Technical field [0001] The invention is the field of microbial testing, which involves a pork virus gene chip and its detection method.The genetic chip includes a probe fixed on the substrate carrier.(PRV), pig ring virus (PCV), pork fine virus (PPV). Background technique [0002] Animal quarantine issues have always been a hot issue that the international community and governments have paid attention to. Especially after joining the WTO in my country, due to the influence of technical trade measures, the hygiene quality requirements for domestic livestock products are getting higher and higher. Many, such as pathogenic bacteria, viruses, etc.At the same time, in order to ensure the quality of food hygiene and protect the health and safety of Chinese consumers, the safety testing of livestock products must be strengthened.The traditional methods currently used in the detection of pig -affected infectious viruses are roughly: virus separation, electron microscope observation, poly...

AI Technical Summary

Problems solved by technology

At present, the traditional methods used for the detection of infectious viruses in pigs generally include: virus isolation method, electron microscope observation, polymerase chain reaction, indirect immunofluorescence test, immunohistochemical technique, enzyme-linked immunosorbent assay, etc. Many defects: The advantage of the polymerase chain reaction (PCR) method is high sensitivity, but the detection of PCR results by gel electrophoresis may lead to false positive judgments due to non-specific amplification, or possible primer amplification band signals Very faint and invisible on gel electrophoresis, causing false negative judgments, and sequencing PCR products is cumbersome and time-consuming

The gene chip detects fewer virus types each time, and the sensitivity is limited, so it is not suitable for large-scale, high-throughput porcine virus detection

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