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Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor

An electrochemical and sensor technology, which is applied in the field of electrochemical sensors for rapid and sensitive determination of tetracycline and its preparation, can solve the problems of expensive instruments, time-consuming and specialized technicians, complex sample derivation processing steps, etc., to improve detection sensitivity and facilitate storage , Accelerate the effect of electron transfer

Active Publication Date: 2022-02-18
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods usually involve disadvantages such as expensive instruments, complicated sample derivatization steps, time-consuming and specialized technicians.

Method used

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  • Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor
  • Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor
  • Electrochemical sensor for measuring tetracycline as well as preparation method and application of electrochemical sensor

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preparation example Construction

[0035] As mentioned in the background art, tetracycline can cause adverse effects on our human body, and it is necessary to further improve the detection selectivity and sensitivity of tetracycline. Based on this, the present invention provides an electrochemical sensor for rapid and sensitive determination of tetracycline, its preparation method and application. The graphene nanobelt in the present invention is a nanobelt made of multi-walled carbon nanotubes, combined with gold nanoparticles by mercapto groups, and then loaded on the graphene nanobelt.

[0036] Graphene nanobelts, a two-dimensional crystalline carbon nanomaterial, have unique advantages in electrochemical sensing and detection due to their good electrical conductivity and electrochemical stability. However, pure graphene nanoribbons are not easily miscible with water, which leads to certain limitations in application. Cyclodextrin molecules have a special structure of "hydrophilic on the outside and hydroph...

Embodiment 1

[0038] Embodiment 1: the preparation of electrochemical sensor

[0039] (1) Preparation of graphene oxide nanobelts. Add 0.1g MWCNT to 150mLH 2 SO 4 / H 3 PO 4 (9:1) in the flask, after stirring for 1 hour, add 0.5g KMnO 4 , the mixture was stirred at 65°C for 2 hours, cooled in an ice bath and added with 0.5 mL of H 2 o 2 , and finally the solution was filtered with a 0.45 μm filter membrane, washed with deionized water several times until the filtrate was neutral, and dried at room temperature for 24 hours.

[0040] (2) Preparation of gold nanoparticles / mercaptocyclodextrin / graphene nanobelts. Dissolve the graphene oxide nanoribbon prepared in step (1) in water and disperse it with ultrasound (power 360w, frequency 40KHz) for 24 hours to obtain a graphene oxide nanoribbon solution. Add 20mg to 40mL water and stir for 0.5 hours to obtain 0.5mg mL -1 mercaptocyclodextrin solution. 40mL graphene oxide nanoribbon solution (0.5mg mL -1 ) and 40mL (0.5mg mL -1 ) SH-β-CD ...

Embodiment 2

[0044] Electrochemical detection of tetracycline on the AuNPs / SH-β-CD-GNRs / GCE prepared in Example 1

[0045] (1) In the potential range of 0.30 ~ 1.10V, in the phosphate buffer solution of pH 6.5, carry out cyclic voltammetry scanning, SH-β-CD-GNRs / GCE and AuNPs / SH-β- prepared in Example 1 CD-GNRs / GCE has an obvious catalytic effect on tetracycline, the results are as follows figure 1 and figure 2 As shown, in comparison, the catalytic effect of AuNPs / SH-β-CD-GNRs / GCE prepared in Example 1 on tetracycline is better.

[0046] (2) In the potential range of 0.30-1.10V, in the phosphate buffer solution of pH 6.5, the enrichment time is 250s, the enrichment potential is 0.30V, and the scanning rate of cyclic voltammetry is changed (5mV / s, 10mV / s s, 20mV / s, 40mV / s, 60mV / s, 80mV / s, 100mV / s), a series of CV curves are obtained. As the scan rate increases, the peak currents of the two oxidation peaks also increase gradually. And the peak current and scan rate of two peaks are lin...

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Abstract

The invention discloses an electrochemical sensor for measuring tetracycline as well as a preparation method and application thereof. The preparation method comprises the steps that a graphene oxide nanobelt is prepared firstly, then a thiolated cyclodextrin / graphene oxide nanobelt solution is prepared, then a HAuCl4 solution is added, an AuNPs / SH-beta-CD-GNRs composite material is prepared by means of the strong reducibility of hydrazine hydrate and the S-Au effect. On the basis, an AuNPs / SH-beta-CD-GNRs / GCE electrode is constructed. As a good electric conductor, the prepared composite material can effectively accelerate electron transfer between an electrode and a solution, so that the detection sensitivity is improved. Under the optimal condition, tetracycline is detected by using linear scanning voltammetry, the linear range is 0.05-240 microns, the detection limit is 0.016 micron (S / N = 3), and good repeatability and selectivity are achieved.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to an electrochemical sensor for rapid and sensitive determination of tetracycline and its preparation method and application. Background technique [0002] Tetracycline is the most common antibiotic used to treat bacterial infections. It can be used for things like urinary tract infections, chlamydia, and acne-like acne on the skin. However, its improper abuse will cause residues in animal-derived foods (such as meat, honey, and chicken, etc.), which will cause harm to human body drug resistance, vision, teeth, and allergies. The Ministry of Agriculture of my country has established corresponding maximum residue limit standards for tetracycline antibiotics that have been approved for use (for example, tetracycline in pork and beef is not higher than 100 μg / kg). Therefore, it is of great significance to develop a sensitive, high-selection and rapid detection method...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 汪小强邓昌晞李铭芳伍忠汉
Owner JIANGXI AGRICULTURAL UNIVERSITY
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