Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Production method of immune electrode

A technology of electrodes and conductive polymers, which is applied in the field of preparation of immune electrodes, can solve the problems that the specificity of antibody-antigen reactions needs to be enhanced, and the stability of electrode modification materials needs to be improved, so as to facilitate a large number of detections, improve detection efficiency and accuracy, The effect of improving sensitivity

Pending Publication Date: 2021-06-18
NANJING LANSION BIOTECH CO LTD
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among them, the electrode is the core of the electrochemical sensor, and nanomaterials or composite materials can be used to prepare modified electrodes, so that the electrodes have more functions, so that they are suitable for a variety of experimental requirements and detection requirements; these materials mainly include: Graphene, graphene-gold nanoparticles, graphene-magnetic gold nanosphere composite materials, graphene-single-stranded DNA, carbon nanotubes-ionic liquids, etc., electrochemical immunosensors based on these materials have high sensitivity, Fast and efficient, etc. are preferred, but the stability as an electrode modification material needs to be improved or the antibody-antigen reaction specificity needs to be enhanced

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method of immune electrode
  • Production method of immune electrode
  • Production method of immune electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1: as Figure 9 As shown, the immune electrode includes a base layer 10, a gold layer 11, a conductive polymer layer 12, an antibody layer 13 and a porous protective layer 14, and the base layer 10, a gold layer 11, a conductive polymer layer 12, an antibody layer 13 and a porous protective layer Layers 14 are laminated sequentially from bottom to top, the base layer 10 is a PET flexible base layer; the conductive polymer layer 12 is a polypyrrole layer.

Embodiment 2

[0068] Embodiment 2: the preparation method based on polypyrrole immune electrode of embodiment 1, it comprises the following steps: (1) synthetic polypyrrole layer (conductive polymer layer): prepare polypyrrole layer on the gold-plated base layer after gold plating, obtain polypyrrole layer pyrrole / gold-plated base layer;

[0069] S11 ultrasonically clean the gold-plated PET base layer with ethanol and ultrapure water in turn, and dry it for later use;

[0070] S12 Fix the dried gold-plated PET base layer on the bottom of a 1000mL beaker, and then add the reaction solution to the beaker, which includes pure water, hydrochloric acid, ferric chloride and pyrrole; first add 400mL of pure water and 6.4 mL1mol / L hydrochloric acid, after stirring evenly, slowly add 0.88g ferric chloride, then stir evenly, place the beaker in ice bath, slowly add 400μL pyrrole under nitrogen atmosphere, and then carry out overnight without light under ice bath nitrogen atmosphere Reaction, the ove...

Embodiment 3

[0080] Embodiment 3: The difference from Example 2 is that the conductive polymer layer in the immune electrode adopts polypyrrole doped with sodium dodecylsulfonate; the preparation method based on polypyrrole immune electrode, its Include the following steps:

[0081] (1) Prepare conductive polymer layer: prepare polypyrrole layer on gold-plated base layer, obtain polypyrrole / gold-plated base layer;

[0082] S11 ultrasonically clean the gold-plated PET base layer with ethanol and ultrapure water in turn, and dry it for later use;

[0083] S12 Fix the gold-plated PET base layer that has been dried for later use at the bottom of a 1000mL beaker, and then add the reaction solution to the beaker, which includes pure water, hydrochloric acid, ferric chloride, pyrrole and sodium dodecylsulfonate (SDS) ; First add 400mL of pure water and 6.4mL of 1mol / L hydrochloric acid to the beaker, after stirring evenly, slowly add 0.88g of ferric chloride and 0.17g of sodium dodecylsulfonate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a production method of an immune electrode. The immune electrode comprises a base layer, a gold layer, a conductive polymer layer and an antibody layer, and the base layer, the gold layer, the conductive polymer layer and the antibody layer are sequentially attached from bottom to top. The production method of the immune electrode specifically comprises the following steps: (1) preparing the conductive polymer layer: preparing a polypyrrole layer on a gold-plated base layer to obtain a polypyrrole / gold-plated base layer; (2) producing the immune electrode: preparing an antibody layer on the polypyrrole layer to obtain an antibody / polypyrrole / gold-plated base layer; and (3) forming an immune electrode system: fixing the bare gold-plated base layer and the outer side of the antibody / polypyrrole / gold-plated base layer to obtain the immune electrode system. The polypyrrole material is used for fixing a biological recognition element antibody, the biological recognition element antibody is immobilized on an immune electrode, and polypyrrole has excellent conductivity so that electrode surface electron transfer can be accelerated, and the sensitivity of the immune electrode is improved.

Description

technical field [0001] The invention belongs to the field of electrochemical immunodetection, in particular to a preparation method of an immunoelectrode. Background technique [0002] Electrochemical immunosensor is an analytical method based on the specific combination of antigen and antibody; it has the advantages of rapid detection, low detection limit, high sensitivity, simple operation and low preparation cost. [0003] In recent years, electrochemical immunosensors have attracted much attention and have been widely used in the detection of tumor markers. When immunosensors are used for continuous target detection, the reproducibility of biomolecular probes is a major problem restricting the practical application of this type of sensors. Therefore, the development of immunosensors that can be mass-produced and low-cost, and used as disposable sensors, will overcome the disadvantages of cumbersome operations, time-consuming and labor-intensive operations caused by the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/531G01N27/327
CPCG01N33/531G01N27/3277G01N27/3278G01N33/5438G01N27/3275B01L3/502707B01L2300/0645B01L2300/0816B01L2300/087G01N27/3276G01N33/547
Inventor 许行尚杰弗瑞·陈
Owner NANJING LANSION BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com