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Bioactive bone meal containing cell sheet and preparation method thereof

A technology of biological activity and cell membrane, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of limited regeneration ability of artificial bone bone cells, safety of allogeneic bone, limited source of autologous bone, etc., and shorten the time of bone repair , promote bone repair, improve the effect of bone regeneration

Pending Publication Date: 2022-02-15
STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of the existing limited sources of autologous bone, potential safety hazards of allogeneic bone, and limited bone cell regeneration ability of artificial bone, and provide a bioactive bone powder containing cell membranes that can improve bone regeneration ability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 A kind of bioactive bone meal containing cell membrane

[0036] The bioactive bone powder containing cell membranes is prepared by the following method:

[0037] S1. Add β-TCP powder into water to make a suspension, and then add an appropriate amount Cellulose and polyethyleneimine (PEI) are dispersed, emulsified and coagulated to form a printing paste, which is printed using a bio-3D printer (Regenovo, China), with a diameter of 2 mm, a pore size of 350-400 μm, and a porosity of 40-55%. , then dried at room temperature for 24 hours, heated at 400°C for 1 hour to remove organic matter, and sintered at 1100°C for 1 hour to obtain a spherical bone meal;

[0038] S2. Human umbilical cord mesenchymal stem cells hUCMSCs were inoculated in osteogenic induction medium, and the inoculation amount was (3~6)×10 4 Human umbilical cord mesenchymal stem cells were cultured and induced for more than 7 days, and then the human umbilical cord mesenchymal stem cells obta...

Embodiment 2

[0040] Embodiment 2 A kind of bioactive bone meal containing cell membrane

[0041] The bioactive bone powder containing cell membranes is prepared by the following method:

[0042] S1. Add β-TCP powder into water to make a suspension, and then add an appropriate amount Cellulose and polyethyleneimine (PEI) are dispersed, emulsified and coagulated to form a printing paste, which is printed using a bio-3D printer (Regenovo, China), with a diameter of 1 mm, a pore size of 400-500 μm, and a porosity of 40-50% , then dried at room temperature for 24 hours, heated at 400°C for 1 hour to remove organic matter, and sintered at 1200°C for 1 hour to obtain a spherical bone meal;

[0043] S2. Human umbilical cord mesenchymal stem cells hUCMSCs were inoculated in osteogenic induction medium, and the inoculation amount was (3~6)×10 4 Human umbilical cord mesenchymal stem cells were cultured and induced for more than 7 days, and then the human umbilical cord mesenchymal stem cells obtaine...

Embodiment 3

[0045] Embodiment 3 A kind of bioactive bone meal containing cell membrane

[0046] The bioactive bone powder containing cell membranes is prepared by the following method:

[0047] S1. Add β-TCP powder into water to make a suspension, and then add an appropriate amount Cellulose and polyethyleneimine (PEI) are dispersed, emulsified and coagulated to form a printing paste, which is printed using a bio-3D printer (Regenovo, China), with a diameter of 3mm, a pore size of 300-400μm, and a porosity of 50-55%. , then dried at room temperature for 24 hours, heated at 400°C for 1 hour to remove organic matter, and sintered at 1000°C for 1 hour to obtain a spherical bone powder;

[0048] S2. Human umbilical cord mesenchymal stem cells hUCMSCs were inoculated in osteogenic induction medium, and the inoculation amount was (3~6)×10 4 Human umbilical cord mesenchymal stem cells were cultured and induced for more than 7 days, and then the human umbilical cord mesenchymal stem cells obta...

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PUM

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Abstract

The invention belongs to the field of biological tissue engineering materials, and particularly relates to bioactive bone meal containing a cell sheet and a preparation method of the bioactive bone meal. The bioactive bone meal is obtained by wrapping 3D-printed spherical bone meal with beta-TCP powder with a cell sheet obtained after human umbilical cord mesenchymal stem cells are subjected to osteogenesis induction and film formation induction, the surface of the bone meal is modified with an extracellular matrix, and the bone meal contains rich cell growth factors, so that the bone meal has biological activity, the bone regeneration capacity can be remarkably improved, the bone repair is promoted, the bone repair time is remarkably shortened, the efficiency of constructing the tissue-engineered bone is improved, and the method is very suitable for the field of bone repair of alveolar bone defects and the like.

Description

technical field [0001] The invention belongs to the technical field of biological tissue engineering materials. More specifically, it relates to a bioactive bone powder containing cell membranes and a preparation method thereof. Background technique [0002] Clinically, tooth defects caused by aging, trauma, inflammation, etc. often bring a lot of inconvenience to the lives of patients. At this time, the restoration of oral dentures is particularly important, and the restoration of oral dentures, especially implant dentures, requires sufficient alveolar bone. However, these patients usually have alveolar bone defect, so the reconstruction of alveolar bone defect has become the research focus of oral restoration. At present, alveolar bone augmentation technologies include: guided bone tissue regeneration technology, bone grafting, bone tissue engineering, etc. Among them, guided bone tissue regeneration is not effective in repairing large-scale bone defects; autologous bon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/50B33Y80/00B33Y10/00
CPCA61L27/3608B33Y80/00A61L27/3834A61L27/3895A61L27/50B33Y10/00A61L2430/02
Inventor 周苗于洋郭凯李树祎耿远明
Owner STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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