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Mineralized artificial periosteum, preparation method and application thereof

A technology of artificial bone and mineralized collagen, which is applied in the fields of medical formula, medical science, prosthesis, etc., can solve the problems of connective tissue ingrowth, achieve good mechanical properties and degradation properties, promote bone healing ability, and improve repair effect Effect

Active Publication Date: 2022-02-11
ASIA BIOMATERIALS WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this application is to provide a mineralized artificial periosteum and its preparation method and application, so as to solve the problem that the existing periosteum is easy to cause the ingrowth of connective tissue

Method used

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  • Mineralized artificial periosteum, preparation method and application thereof
  • Mineralized artificial periosteum, preparation method and application thereof
  • Mineralized artificial periosteum, preparation method and application thereof

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preparation example Construction

[0054] According to a typical embodiment of the present invention, a preparation method of mineralized artificial periosteum is provided, the method comprising:

[0055] S1, the preparation of dense layer, specifically include:

[0056] S1-1. Dissolving silk fibroin in lithium bromide solution or calcium chloride ternary system (calcium chloride / water / ethanol molar ratio 1:8:2) solution to obtain silk fibroin with a mass fraction of 5% to 20% protein solution.

[0057] The silk fibroin is selected from the natural high-molecular fiber protein extracted from silkworm cocoons or silk, and the mass fraction of silk fibroin in the silk fibroin solution is 5% to 20%. The reason: too high mass fraction of silk fibroin is not conducive to Degradation is slow during dissolution and use, and the low mass fraction affects the mechanical properties and physical barrier function of the membrane;

[0058] S1-2, taking the silk fibroin solution obtained in step S1-1 and injecting it into ...

Embodiment 1

[0096] A preparation method of mineralized artificial periosteum, the method comprising:

[0097] S1, the preparation of dense layer, specifically include:

[0098] S1-1. Dissolving silk fibroin in lithium bromide solution or calcium chloride ternary system (calcium chloride / water / ethanol molar ratio 1:8:2) solution to obtain a silk fibroin solution with a mass fraction of 5%.

[0099] S1-2. Take the silk fibroin solution obtained in step S1-1 and inject it into the mold, pre-freeze at -60°C for 12 hours, then dry the frozen body at a temperature of 10°C and a pressure of 10Pa for 48 hours, and then mold it under a pressure of 20MPa 60s to obtain dense layer film;

[0100] Thermally cross-linking the dense layer film at 110° C. for 24 hours in a vacuum oven to obtain a dense layer;

[0101] S2, the preparation of mineralized collagen solution, specifically include:

[0102] S2-1. Dissolving type I collagen in an acetic acid solution with a mass fraction of 5%, wherein the c...

Embodiment 2

[0111] A preparation method of mineralized artificial periosteum, the method comprising:

[0112] S1, the preparation of dense layer, specifically include:

[0113] S1-1. Dissolving silk fibroin in lithium bromide solution or calcium chloride ternary system (calcium chloride / water / ethanol molar ratio 1:8:2) solution to obtain a silk fibroin solution with a mass fraction of 5%.

[0114] S1-2. Take the silk fibroin solution obtained in step S1-1 and inject it into the mold, pre-freeze at -60°C for 12 hours, then dry the frozen body at a temperature of 10°C and a pressure of 10Pa for 48 hours, and then mold it under a pressure of 20MPa 30s to obtain dense layer film;

[0115] Thermally cross-linking the dense layer film at 110° C. for 24 hours in a vacuum oven to obtain a dense layer;

[0116] S2, the preparation of mineralized collagen solution, specifically include:

[0117] S2-1. Dissolving type I collagen in an acetic acid solution with a mass fraction of 5%, wherein the c...

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Abstract

The invention particularly relates to a mineralized artificial periosteum, a preparation method and application thereof, and belongs to the technical field of biomedical materials, wherein a compact layer is prepared firstly, and then a loose layer is prepared on the compact layer. According to the invention, a mineralized artificial periosteum with a double-layer structure is constructed, wherein a compact layer and a loose layer are synergistically combined, the surface of the compact layer is smooth, and the compact layer faces soft tissue during use, so that the physical barrier function of the periosteum can be well utilized, connective tissue is effectively prevented from growing into a bone defect area, and the regeneration function of specific tissue is played to the maximum extent; the rough loose layer is consistent with the autogenous bone in component and structure, faces bone tissue during use, and can be well attached to a bone defect surface and induce new bone generation, so that the repair effect is improved; and the mineralized artificial periosteum obtained by the method has good mechanical properties and degradation properties, and also has good bone healing promoting ability.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, in particular to a mineralized artificial periosteum and its preparation method and application. Background technique [0002] Periosteum is a dense connective tissue membrane that covers almost all bone surfaces except joints. It consists of an outer fibrous layer and an inner cambium. It plays an important role in bone development and fracture healing. The fibrous layer contains fibroblasts, collagen and elastic fibers, a network of nerves and microvessels, and provides the mechanical stability of the periosteum. The cambium stores a large number of different types of cells and provides a source of cells for bone formation and repair. Periosteum can provide nutrients to bone tissue through blood vessels, induce bone growth, control the direction of bone growth, etc., and play a vital role in the repair of bone tissue defects. Numerous studies have demonstrated the importance of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/22A61L27/24A61L27/12A61L27/50A61L27/54A61L27/58
CPCA61L27/227A61L27/24A61L27/12A61L27/50A61L27/54A61L27/58A61L2430/02A61L2400/12A61L2300/412A61L2300/112A61L2300/252C08L89/00
Inventor 唐三周雄王喆
Owner ASIA BIOMATERIALS WUHAN CO LTD
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