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Medicine for preventing and treating ischemic heart disease of mammals as well as preparation method and application of medicine

A technology of ischemic heart disease and mammals, applied in the field of medicine, to avoid fatal arrhythmia, improve the microenvironment, and improve the effect of survival

Inactive Publication Date: 2022-02-11
杭州科贝生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no drug or method that can promote the regeneration of coronary collateral circulation in the ischemic area of ​​the myocardium to rebuild the damaged coronary vasculature

Method used

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  • Medicine for preventing and treating ischemic heart disease of mammals as well as preparation method and application of medicine
  • Medicine for preventing and treating ischemic heart disease of mammals as well as preparation method and application of medicine
  • Medicine for preventing and treating ischemic heart disease of mammals as well as preparation method and application of medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: preparation of pharmaceutical composition

[0040]Collect the whole herb of Lanbuzhenghe Potentilla at an altitude of 1000-1400 meters in Guizhou, wash it, and dry it in the shade; or collect the whole herb of fresh Lanbuzhenghe Potentilla at an altitude of 1000-1400 meters in Guizhou and wash it. The above-mentioned fresh grass freeze-dried / natural shade-dried plant raw materials were respectively cut into pieces, and 80% of the blue cloth was mixed with 20% of Potentilla. In the experiment, it was found that the ethanol / water extract of Lanbuzheng not only contains components that induce coronary vascular regeneration in the ischemic area of ​​the heart and rebuilds the cardiac vascular network, but also contains components that damage the liver. And it was found that the liver-damaging component also inhibits the active site from regenerating myocardial tissue with the correct ultrastructure at the site of cardiac injury. In order to remove the liver-h...

Embodiment 2

[0044] Embodiment 2: Promote the formation of blood vessel-like lumen in 3D cell culture of vascular endothelial cells

[0045] The preparation of Ham's F-12 three-dimensional vascular endothelial cell culture medium is briefly as follows: Fibrin was prepared by polymerization of α-thrombin (2.5 units / ml) and fibrinogen solution (5 mg fibrinogen / ml serum-free medium) matrix. After polymerization, the gel was immersed in Ham's F-12 medium containing 10% calf serum and incubated in a 37ºC water bath for 3 hours to inactivate α-thrombin. The active site composition was determined in a three-dimensional vascular endothelial cell culture medium to promote the formation of blood vessel-like lumen structures of human coronary artery endothelial cells (HCAEC) in the culture medium. Inoculate HCAEC on the surface of the three-dimensional culture matrix in a 96-well culture dish, add different concentrations (5-30μg / ml) of the active site composition to treat the target cultured cells,...

Embodiment 3

[0048] Example 3: Animal model of myocardial infarction evaluates the composition of the active site, that is, the pharmaceutical composition

[0049] The therapeutic effect of

[0050] The animal model of myocardial infarction was made by completely ligating the anterior descending coronary artery in rats. Thirty male Sprague-Dawley rats with a body weight of 300-350 grams were injected intraperitoneally with pentobarbital (50 mg / kg body weight) at room temperature to anesthetize the experimental animals, carried out endotracheal intubation and carried out artificial respiration with a small animal ventilator, The left anterior descending (LAD) artery of the heart of the experimental animal was surgically opened, permanently ligated completely, the chest was closed, and the animal was placed in a warm area to wake up and recover to create an animal model of myocardial infarction on the anterior wall of the left ventricle. Three days after LAD ligation, aCGJ (250 mg / kg body w...

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Abstract

The invention discloses a medicine for preventing and treating ischemic heart disease of mammals and a preparation method and application thereof. The present invention relates to the method for more significantly increasing the effect of preventing or treating ischemic heart disease (IHD) in mammals, including human beings, by removing hepatotoxic parts from fresh plants aleppo avens and potentilla chinensis, and significantly increasing the effective content of active compounds. The IHD or disease states include chronic coronary heart disease, acute myocardial infarction and heart failure diseases caused by acute myocardial infarction. The active sites provided by the invention can be formulated as pharmaceutical compositions and medicaments for use in the disclosed methods. Also provided are uses of the active sites in the preparation of pharmaceutical preparations and medicaments.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a medicine for preventing and treating ischemic heart disease in mammals. [0002] Materials and preparation methods and applications. Background technique [0003] The heart is an important internal organ and the organ with the largest oxygen consumption per unit. The myocardial blood flow per 100g is about 60-80ml / min. If the adult heart is 300g (about 0.5% of the body weight), the overall myocardial blood flow is about 180-80ml / min. 240ml / min. In contrast, the average blood flow of the whole body tissue is 7ml / min per 100g, and the heart is about 10 times more. Myocardial oxygen uptake capacity is very strong, it can take up about 65-75% of oxygen from capillaries. % oxygen, the average oxygen uptake per 100g of other tissues is only about 0.3ml / min. During exercise, the cardiac output needs to increase significantly, the workload of the heart increases proportionally, and the oxyg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P9/10A61P9/04
CPCA61K36/73A61K9/0053A61K9/006A61K9/0019A61P9/10A61P9/04A61K2236/35A61K2236/37A61K2236/333A61K2236/51A61K2236/55A61K2300/00
Inventor 李明梅丽萍
Owner 杭州科贝生物制药有限公司
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