A personalized 3D printed meniscal regeneration scaffold and its preparation method

Abstract

The invention provides a method for preparing a personalized 3D printed meniscus regeneration bracket, comprising: S1, preparing a bracket model and setting a support structure model for supporting the bracket model; S2, setting a high-temperature print head on a biological 3D printer, and low-temperature printing The working parameters of the printing design of the head and internal structure; S3, the extruded material in the high-temperature print head and the extruded material in the low-temperature print head are mixed and printed, and the inner 2 / 3 area of ​​​​the meniscus regeneration bracket is set as the "white area", The outer 1 / 3 area was set as the "red area" to construct the meniscus regeneration scaffold by partition printing. The advantages are: the bottom of the meniscus bracket can match the tibial plateau; and the regeneration and repair function after implantation can be effectively enhanced.

Description

technical field [0001] The invention relates to the technical field of medical prosthesis, in particular to a personalized 3D printed meniscus regeneration bracket and a preparation method thereof. Background technique [0002] The meniscus is a semilunar-shaped fibrocartilage tissue located between the femoral condyle and the tibial plateau cartilage. In the knee joint, it performs functions such as cushioning and decompression, absorbing shock, lubricating the joint, and maintaining joint stability. The blood supply of the joint capsule and synovium is called the "red zone". The inner 2 / 3 area lacks blood supply and only provides nutrition through the penetration of joint fluid, which is called the "white zone". [0003] As the number of people currently participating in sports continues to increase, the incidence of meniscus injuries is also increasing accordingly. Meniscal injury can significantly affect its function. Mild cases manifest as asymptomatic or mild joint pa...

AI Technical Summary

Problems solved by technology

However, most of the brackets produced in the previous research did not restore the shape of the original meniscus 1:1, and there were still some differences in shape with the natural meniscus, so they could not match the tibial plateau at the bottom and the femoral condyle above it, resulting in easy meniscus brackets. Dislocation, or uneven distribution of stress, ultimately affecting meniscal regeneration

Moreover, different growth factors can promote cells to produce different phenotypes and secrete different components. However, in previous studies, seed cells and growth factors were mostly evenly distributed throughout the scaffold, which eventually led to the regeneration of meniscus tissue in collagen composition, cell The phenotype is relatively single, which is quite different from the heterogeneous distribution of the natural meniscus, and cannot meet the functions of the natural meniscus

Ultimately, the regenerated tissue-engineered meniscus still has a large difference in collagen composition or distribution from the natural meniscus, or the regenerated meniscal tissue has poor cartilage protection

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