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Feed additive for enriching phosphatidyl ethanolamine DHA in eggs, and preparation method and application thereof

A technology for enriching phosphatidylethanolamine type and phosphatidylethanolamine type, which is applied in the application field of laying hen feed, can solve the problems of low purity of DHA, inapplicability, instability of phosphatidylethanolamine type DHA, etc., and achieve the goal of reducing the difficulty of preparation Effect

Active Publication Date: 2022-01-28
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the feed structure of chickens and other reasons, most of the DHA enriched in eggs is of the phosphatidylcholine type, which makes it impossible for people to eat this kind of eggs to achieve the purpose of nourishing the brain efficiently.
[0005] (2) The purity of DHA is low. There are many ways to enrich DHA in eggs. Among them, adding flaxseed (oil) and fish oil to the diet of chickens can easily enrich DHA in eggs, but at the same time it will also cause the accumulation of DHA in eggs. The content of eicosapentaenoic acid (EPA) in eggs is too high, and EPA will be converted into prostaglandins after being eaten by infants, which will have a certain impact on the body of infants and young children. Therefore, eggs with high EPA content are not suitable for mothers. infant
[0006] (3) Phosphatidylethanolamine-type DHA is unstable. In DHA eggs produced by traditional methods, because phosphatidylethanolamine-type DHA has a very strong reducing property, it is easily oxidized, causing phosphatidylethanolamine-type DHA to appear in eggs. Stable phenomenon, causing its content in eggs to be high and low, or even absent from time to time

Method used

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  • Feed additive for enriching phosphatidyl ethanolamine DHA in eggs, and preparation method and application thereof
  • Feed additive for enriching phosphatidyl ethanolamine DHA in eggs, and preparation method and application thereof
  • Feed additive for enriching phosphatidyl ethanolamine DHA in eggs, and preparation method and application thereof

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Experimental program
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Effect test

preparation example

[0037]The following Schizochytrium species were purchased from the US ATCC, number 20888. Alkaline protease is a serine protease purchased from Wuhan Jinke Tiancheng Technology Co., Ltd. Soybean phospholipid powder, yeast selenium and cysteine ​​were purchased from Hunan Cannon Zhenghe Biotechnology Co., Ltd.

[0038] The preparation method of directional purified soybean lecithin is as follows: take 1 weight part of soybean lecithin powder and put it into a reaction kettle, add 18 parts by weight of absolute ethanol, and 1.5 parts by weight of ammonia water with a mass concentration of 20%, and stir at 45°C at 80 rpm React for 50 minutes. After the reaction, the filtrate was obtained by centrifugation at 500×g for 15 minutes, and the filtrate was freeze-dried at -20° C. for 8 hours to obtain directional purified soybean lecithin.

[0039] The preparation method of the broken-wall Schizochytrium powder is as follows: the Schizochytrium algae species are placed in an aqueous ...

Embodiment 1

[0041] 1. Experimental design

[0042] The experiment selected a single-factor randomized block test design, and randomly selected 600 healthy Dawu Golden Phoenix chickens with similar ages (220 ± 10 days) and similar egg production rates and divided them into 6 groups randomly, with 4 repetitions in each group. 25 chickens per replicate, where:

[0043] (1) Group C1 is a blank control group, fed with a basal feed that does not contain any DHA and additives related to the present invention as a basal diet. The basic feed is conventional laying hen feed produced by Changsha Weijia Feed Co., Ltd.;

[0044] (2) Group C2 was the directional purified soybean phospholipid-deficient control group, and added 0.33% broken Schizochytrium powder + 0.03% yeast selenium + 0.22% cysteine ​​on the basis of C1 feed. That is, the quality of the basic feed is 100 parts by weight, the addition of the broken wall Schizochytrium powder is 0.33 parts, the addition of yeast selenium is 0.03 parts...

Embodiment 2

[0058] On the basis of the preparation method of T group directional purification soybean lecithin in embodiment 1, change the source of directional purification soybean lecithin and / or processing method, experimental design is as follows:

[0059] Group A1: no ethanol was added during the preparation of directional purified soybean lecithin;

[0060] Group A2: no ammonia water was added during the preparation of directional purified soybean lecithin;

[0061] Group A3: The preparation process is the same as that of Group T, and ethanol and ammonia water are added during the preparation process of directional purified soybean lecithin;

[0062] Group A4: the raw material was replaced with fresh soybean oil residue, extracted with ethanol;

[0063] Group A5: the raw material is replaced with fresh soybean oil residue, extracted with ammonia water;

[0064] Group A6: the raw material is replaced with fresh soybean oil residue, extracted with ethanol and ammonia water;

[0065...

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Abstract

The invention provides a feed additive capable of enriching phosphatidyl ethanolamine DHA in eggs. The feed additive contains directionally purified soybean phospholipids, wall-broken schizochytrium limacinum powder, selenium yeast and cysteine. Wherein the directionally purified soybean phospholipid is rich in phosphatidyl ethanolamine soybean phospholipid, the schizochytrium limacinum provides DHA, selenium yeast and cysteine which can generate a certain content of glutathione peroxidase in chicken bodies, full combination of phosphatidyl ethanolamine and DHA is guaranteed, the structure and content of phosphatidyl ethanolamine DHA are stabilized, and phosphatidyl ethanolamine DHA is stably enriched in eggs. The directionally purified soybean phospholipid, the wall-broken schizochytrium limacinum powder, the selenium yeast and the cysteine are powder and are easy to mix uniformly, so that the preparation method is different from the prior art in which oily substances such as schizochytrium limacinum oil are adopted, and the preparation difficulty is reduced.

Description

technical field [0001] The invention relates to the technical field of feed additives and laying hen feeding, in particular to a feed additive for enriching eggs with phosphatidylethanolamine-type DHA, a preparation method of the feed additive, and its application in laying hen feed. Background technique [0002] Docosahexaenoic acid, or DHA for short, is known as brain gold because of its huge proportion in the brain and its ability to promote brain development in infants and young children. In addition to its important role in the human brain, DHA also plays an indispensable role in the development of the optic nerve and the improvement of immunity. In the DHA supplements currently on the market, the types of DHA are not uniform, such as ethyl ester type, triglyceride type, phospholipid type, etc., and phospholipid type DHA includes phosphatidylcholine type (lecithin type) DHA and phosphatidylethanolamine (cephalin) DHA. In the absorption and utilization of DHA in the br...

Claims

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Application Information

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IPC IPC(8): A23K50/75A23K20/158A23K20/20A23K20/142A23K10/16A23K10/30A23K10/37
CPCA23K50/75A23K20/158A23K20/30A23K20/142A23K10/16A23K10/30A23K10/37Y02P60/87
Inventor 孙志良张运强
Owner HUNAN AGRICULTURAL UNIV
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