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Chaetomium globosum and application of chaetomium globosum in preparation of medicament for preventing and treating wheat sharp eyespot

A kind of technology of Chaetomium globulus, Chaetomium spores, applied in the field of microorganisms

Active Publication Date: 2021-12-31
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Chaetomium globosa is an important flora in the genus Chaetomium, belonging to the subphylum Ascomycota (Aseomyeotina), class Pyrenomyeetes, order Sphaeriales ), Melanosporaceae, and Chaetomium spp. are common plant endophytic fungi that are widely distributed in various natural environments such as air and soil. These microorganisms can colonize plant roots, Promote the nutrient absorption of plants, and have a good antagonistic effect on a variety of plant pathogens. At present, there is no report on the control of wheat sheath blight in the field by Chaetomium globosa

Method used

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  • Chaetomium globosum and application of chaetomium globosum in preparation of medicament for preventing and treating wheat sharp eyespot
  • Chaetomium globosum and application of chaetomium globosum in preparation of medicament for preventing and treating wheat sharp eyespot
  • Chaetomium globosum and application of chaetomium globosum in preparation of medicament for preventing and treating wheat sharp eyespot

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The separation of embodiment 1 Chaetomium globosa strain

[0021] At the wheat grain filling stage, the uninfected plants in the wheat field were collected in Changge County, Henan Province, and put into paper bags. The samples were stored in a refrigerator at 4°C. Rinse the root of the sample, and then take 2-3cm of wheat root tissue, disinfect the surface with 75% absolute ethanol for 10-30s, sterilize with 1% NaClO for 1.5min, rinse with sterile water for 3 times, and blot dry with sterile filter paper After hydration, cut into small sections of about 0.5 cm, and place them on a potato dextrose agar medium (PDA, raw material composition: 300 g of peeled potatoes, 20 g of glucose, 20 g of agar and 1000 ml of distilled water) with sterile tweezers. After 5-7 days of under-cultivation, the edge mycelia were picked and grown on a new PDA plate. After 5-7 days under the same conditions, the mycelium pieces with uniform colonies were picked and cultured on a PDA slant, an...

Embodiment 2D

[0022] Example 2DNA Extraction

[0023] Pick the mycelium pieces onto the PDA medium, and after culturing at 20°C for 5-7 days, pick 5 edge mycelium pieces and place them evenly on a PDA plate covered with sterilized cellophane. After 3-5 days, scrape the mycelium with a sterilized small shovel, freeze it with liquid nitrogen, and store it in a refrigerator at -20°C. When extracting DNA, take out 20-25mg mycelium and put it into a pre-cooled 1.5ml EP tube; add a little liquid nitrogen and grind the mycelium to powder in the EP tube with a pre-cooled iron nail; add 500μL Extraction Buffer (50mM Tris- Cl (pH 8.0), 150mM NaCl, 100mM EDTA (8.0)), oscillated on a vortex shaker to suspend the precipitate and mix well; Mix upside down, place in a water bath at 37°C for 1-3h; then add 75 μL of 5M NaCl, mix upside down; add 65 μL of CTAB / NaCl (10% CTAB, 0.7M NaCl) solution, bathe in water at 65°C for 30 min; add etc. volume (700 μL) of Tris-phenol: chloroform: isoamyl alcohol (25:24:...

Embodiment 3

[0024] Example 3 identification

[0025] This test uses primers ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3', as shown in SE ID NO.1) and ITS4 (5'-TCC TCC GCT TAT TGA TAT GC-3', as shown in SEQ ID NO. .2) to amplify the DNA fragments containing ITS1, 5.8S rDNA and ITS2 of the strain to be tested. The system refers to Daval et al. (2010). Amplification reaction conditions: PCR reaction program: 95°C for 3min; 95°C for 45s, 50°C for 30s, 72°C for 1min, 35 cycles; 72°C for 10min. After the PCR product was sequenced, the BLAST sequence alignment analysis was carried out in NCBI, and the sequence alignment result showed that the strain was Chaetomium globosa. The accession number of the ITS sequence on NCBI is OK287116, and the strain was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation date is August 4, 2020, and the preservation number is CGMCC No.19939. The preservation address is: No. 3, No. 1 Yard, Beich...

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Abstract

The invention discloses chaetomium globosum, which is a chaetomium globosum (Chaetomiumglobosum) 12CG5-11 strain, is preserved in China General Microbiological Culture Collection Center on August 4, 2020, has a preservation number of CGMCC No.19939, has a preservation address of No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and is named as chaetomium globosum (Chaetomiumglobosum) in taxonomy. The chaetomium globosum spore liquid is produced through fermentation of a millet grain culture medium, the chaetomium globosum spore liquid is applied to preparation of a medicament for preventing and treating wheat sharp eyespot, and field test results show that after the chaetomium globosum 12CG5-11 spore liquid is sprayed in the sowing period of wheat, the prevention effects on the wheat sharp eyespot in the jointing period and the mature period reach 47.83% and 53.40% respectively.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a chaetomium globosa and its application in preparing medicaments for preventing and controlling wheat sheath blight. Background technique [0002] The use of microbial inoculum seed dressing to promote plant growth is a hot research direction at present. Endophytic fungi in plants, as a new microbial resource and the natural active substances they produce, have received more and more attention. [0003] Chaetomium globosa is an important flora in the genus Chaetomium, belonging to the subphylum Ascomycota (Aseomyeotina), class Sclerotia (Pyrenomyeetes), order Sphaeriales, family Melanosporaceae, genus Chaetomycetes ( Chaetomium spp.), is a common plant endophytic fungus, which is widely distributed in various natural environments such as air and soil. This type of microorganism can colonize plant roots, promote plant nutrient absorption, and has a good ef...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30
Inventor 冯超红李丽娟张姣姣宋玉立徐飞王俊美李亚红韩自行刘露露石瑞杰
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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