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Rapid determination method suitable for ochratoxin A in licorice paste

A technology for the rapid determination of ochratoxin, applied in the preparation of test samples, measuring devices, instruments, etc., can solve the problem of inability to effectively dissolve licorice paste to extract ochratoxin A, difficulty in accurately determining ochratoxin A, pretreatment and purification There are many impurity steps, etc., to achieve the effect of short purification treatment time, removal of impurity interference, and less impurity interference

Pending Publication Date: 2021-12-17
乌鲁木齐海关技术中心
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  • Claims
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AI Technical Summary

Problems solved by technology

[0007] In view of the fact that no effective method for the determination of ochratoxin A in licorice paste has been found so far, the existing methods for the detection of ochratoxin A cannot effectively dissolve licorice paste and extract ochratoxin A, and there are many steps for pre-treatment to purify impurities. High cost and cumbersome operation, poor purification effect, leading to the technical status quo that cannot be accurately quantified, the present invention aims to provide a fast and effective detection method suitable for ochratoxin A in licorice paste
The detection method suitable for ochratoxin A in licorice paste provided by the present invention effectively solves the problem that the existing method is difficult to accurately determine ochratoxin A in licorice paste, and establishes a low-cost, accurate, fast and effective detection method for ochratoxin A in licorice paste. The determination method of aspergillus toxin A content provides an effective detection technology for the determination of ochratoxin A content in licorice paste

Method used

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  • Rapid determination method suitable for ochratoxin A in licorice paste
  • Rapid determination method suitable for ochratoxin A in licorice paste
  • Rapid determination method suitable for ochratoxin A in licorice paste

Examples

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Embodiment 1

[0026] Embodiment 1: The rapid determination method that is applicable to licorice paste ochratoxin A

[0027] A kind of rapid determination method applicable to ochratoxin A of licorice plaster, specifically comprises the following steps:

[0028] (1) Weigh 1.0 g of licorice paste sample, add 20 mL of extract, vortex and sonicate at 60°C for 30 minutes, take out the vortex 3 times during the period, cool to room temperature after sonication, and centrifuge at 8000 rpm 5min, take 10mL of supernatant in a centrifuge tube, add 5mol / L hydrochloric acid to adjust the pH to 2-3, vortex for 0.5min, add 15mL of chloroform, shake for 3min, centrifuge and take the chloroform layer into a chicken heart bottle , repeat the extraction once, combine the extracts and evaporate to 1-2mL at 50°C, add 20mL of extracts and vortex for 1min, then transfer the whole solution in the chicken heart bottle to a clean centrifuge tube, shake for 5min, and spin at 8000rpm After centrifugation for 5min, ...

Embodiment 2

[0033] Example 2: Application of Rapid Determination Method for Ochratoxin A in Licorice Paste

[0034] The present embodiment, on the basis of Example 1, to the actual application effect of the assay method applicable to licorice paste ochratoxin A provided by the present invention, to the purification of immunoaffinity column (contrast 1) and ion exchange solid phase in the prior art Extraction column purification (control 2), phenylsilane solid-phase extraction column and immunoaffinity column were purified twice to determine ochratoxin A in licorice paste samples (control 3), and the literature (“liquid-liquid extraction combined with immunoaffinity Column Chromatography Purification-High Performance Liquid Chromatography Determination of Ochratoxin A in Roasted Coffee", Fan Xiang et al., Physicochemical Test-Chemical Volume, 2008,44(8):736-739) reported a kind of technical scheme for the determination of ochratoxin A in licorice paste The assay results of ochratoxin A (co...

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Abstract

The invention discloses a rapid and accurate detection method suitable for ochratoxin A in liquorice paste. The rapid and accurate detection method suitable for the ochratoxin A in the licorice paste is finally obtained through key control steps of selecting an extraction solvent, optimizing liquid-liquid extraction and back extraction purification, improving a leaching solution of immunoaffinity column purification and the like. When an extracting solution is prepared from a NaHCO3 solution with the concentration of 30 g / L and acetonitrile according to a volume ratio of 60:40, the liquorice paste is completely dissolved, the pH value is adjusted to be 2-3, trichloromethane is used for extraction, then an extracting solution back-extraction technology is used for purification, an immunoaffinity column is used for re-purification, then determination is conducted, and the method is simple and rapid, good in purification effect, capable of effectively removing impurity interference and high and stable in recovery rate. The detection method provided by the invention is used for detecting the ochratoxin A in the licorice paste, the recovery rate 9 is as high as 0%, the problem that the ochratoxin A in the licorice paste is difficult to rapidly and accurately determine by the existing method is effectively solved, and the low-cost, rapid and accurate determination method for content of the ochratoxin A in the licorice paste is established.

Description

[0001] field of invention [0002] The invention relates to a detection method of ochratoxin A, in particular to a rapid detection method suitable for ochratoxin A in licorice paste. Background technique [0003] In the process of harvesting, processing, transportation and storage, Chinese herbal medicines may infect various fungi, especially toxin-producing fungi, under the influence of their own factors and external environmental conditions. and the quality and safety of its products. Mycotoxins do not show acute toxicity to the body, but they have a strong accumulation in the body, damage liver and kidney functions, and seriously threaten human health and even life safety. There are many kinds of Chinese herbal medicines, and the mildew conditions are different. The pollution and residual levels of mycotoxins and their impact on the quality of medicinal materials are also quite different. [0004] Ochratoxin A (OTA for short) is a toxic metabolite produced by several fung...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/08G01N30/14G01N30/74G01N1/38
CPCG01N30/06G01N30/08G01N30/14G01N30/74G01N1/38G01N2030/062G01N2030/146
Inventor 巩志国房芳苏敏王静静何璐齐冬琴姚伟琴李晓岩彭静怡
Owner 乌鲁木齐海关技术中心
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