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Hair follicle tissue composite preserving fluid, preparation method thereof and preserving method for maintaining in-vitro activity of hair follicles

A technology for preserving solution and hair follicles, which is applied in the field of maintaining the in vitro activity of hair follicles, and in the field of composite preservation solution for hair follicle tissue, which can solve the problems of lack of protective components, unfavorable in vitro preservation of hair follicles, cell edema, etc., and achieve the effect of good clinical application prospects.

Active Publication Date: 2021-12-10
北京大麦植发技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its high sodium properties may lead to cell edema at low temperature. In addition, due to its lack of protective components, it is not conducive to long-term preservation of hair follicles in vitro, so it is not the best choice for in vitro preservation.

Method used

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  • Hair follicle tissue composite preserving fluid, preparation method thereof and preserving method for maintaining in-vitro activity of hair follicles
  • Hair follicle tissue composite preserving fluid, preparation method thereof and preserving method for maintaining in-vitro activity of hair follicles
  • Hair follicle tissue composite preserving fluid, preparation method thereof and preserving method for maintaining in-vitro activity of hair follicles

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1: A kind of hair follicle tissue composite preservation solution and the preservation method of maintaining the activity of isolated hair follicles:

[0068] 1. A hair follicle tissue composite preservation solution in this embodiment is composed of a basic preservation solution and an auxiliary preservation solution, wherein the basic preservation solution is composed of a solute, a solvent and a pH regulator;

[0069] The pH adjuster is sodium hydroxide / hydrochloric acid, which is used to adjust the pH value of the hair follicle tissue composite preservation solution to 7.4;

[0070] The solvent is medical sterile water; a part of the solvent is used to dissolve the solute and configure it into a solution, and the volume of the solution is 1 / 2 of the volume of the hair follicle tissue composite preservation solution; the other part of the solvent is used to constant the volume of the solution added with the components of the auxiliary preservation solution ...

Embodiment 2

[0093] Embodiment 2: A kind of hair follicle tissue composite preservation solution and the preservation method for maintaining the activity of isolated hair follicles:

[0094] The content of each component of solute in the present embodiment is:

[0095] Sodium chloride 5 mmol / L

[0096] Potassium chloride 10 mmol / L

[0097] Magnesium chloride hexahydrate 1 mmol / L

[0098] Lacturonic acid 25 mmol / L

[0099] Trehalose 5 mmol / L

[0100] Mannitol 1 mmol / L

[0101] Potassium dihydrogen phosphate 5 mmol / L

[0102] The content of each component in the auxiliary preservation solution in the present embodiment is:

[0103] Histidine 1 mmol / L

[0104] Tryptophan 1 mmol / L

[0105] Glutamine 1 mmol / L

[0106] Glutathione 1 mmol / L

[0107] Adenosine triphosphate 1 mmol / L

[0108] Desfermine 5 umol / L

[0109] Insulin 3 IU / L

[0110] Hydrocortisone 4 mg / L

[0111] The preparation method of the hair follicle tissue composite preservation solution and the preservation method f...

Embodiment 3

[0112] Embodiment 3: a kind of hair follicle tissue composite preservation solution and the preservation method of maintaining the activity of isolated hair follicles:

[0113] The content of each component of solute in the present embodiment is:

[0114] Sodium chloride 30 mmol / L

[0115] Potassium chloride 20 mmol / L

[0116] Magnesium chloride hexahydrate 10 mmol / L

[0117] Lacturonic acid 75 mmol / L

[0118] Trehalose 20 mmol / L

[0119] Mannitol 50 mmol / L

[0120] Potassium dihydrogen phosphate 50 mmol / L

[0121] The content of each component in the auxiliary preservation solution in the present embodiment is:

[0122] Histidine 100 mmol / L

[0123] Tryptophan 20 mmol / L

[0124] Glutamine 20 mmol / L

[0125] Glutathione 50 mmol / L

[0126] Adenosine triphosphate 50 mmol / L

[0127] Desfermine 50 umol / L

[0128] Insulin 50 IU / L

[0129] Hydrocortisone 60 mg / L

[0130] The preparation method of the hair follicle tissue composite preservation solution and the preserva...

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Abstract

The invention discloses a hair follicle tissue composite preserving fluid, a preparation method thereof and a preserving method for maintaining in-vitro activity of hair follicles, and belongs to the technical field of cell cryopreservation. The preserving method for maintaining in-vitro activity of hair follicles comprises the following steps: 1, taking a hair follicle tissue composite preservation solution, and pre-cooling at 4 DEG C; taking in-vitro unit hair follicles, rinsing the in-vitro unit hair follicles with a Ringer's solution, and removing blood clots and impurities; the rinsed unit hair follicles are placed in a cold hair follicle tissue composite preserving fluid to be fully immersed, and the preserving temperature needs to be maintained at 4 DEG C; 2, removing the hair follicle tissue composite preserving fluid, rinsing unit hair follicles with Ringer's solution, and transplanting the hair follicles into a human body after removing residual preserving fluid. The hair follicle tissue composite preserving fluid is composed of a basic preserving fluid and an auxiliary preserving fluid, wherein the basic preserving fluid is composed of a solute, a solvent and a pH value regulator. The method has the advantages that no risk doubt component exists, the stored hair follicle can be directly used for clinical transplantation treatment after being simply flushed with normal saline, and the method is safe and effective and has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of cell cryopreservation, and in particular relates to a hair follicle tissue composite preservation solution, a preparation method thereof and a preservation method for maintaining hair follicle activity in vitro. Background technique [0002] Androgenic alopecia (AGA) is one of the most common skin diseases in the world, afflicting more than 100 million adult men in my country. Although AGA does not affect physical health, its bald appearance has a great psychological impact on patients, and some patients even have social barriers. As an important means of clinical treatment of AGA, surgical hair transplantation is mainly aimed at severe and late irreversible AGA hair loss. With the development of technology, it is also suitable for mild to moderate patients. At present, the main surgical treatment plan is to drill the healthy unit hair follicles of the posterior occipital, and then transplant them to the...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 李兴东胡志奇苗勇周易
Owner 北京大麦植发技术研究有限公司
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