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Dominant epitope peptide of Ebola virus envelope glycoprotein as well as coding gene and application thereof

A technology of Ebola virus and envelope glycoprotein, which is applied in the field of dominant epitope peptide of Ebola virus envelope glycoprotein, and achieves the effect of wide application prospect and safe and convenient use

Active Publication Date: 2021-11-30
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only peptides with high affinity and tight binding to MHC class I molecules can be expressed on the surface of APC cells and presented to CD8+ T cells for recognition, thereby triggering an immune response

Method used

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  • Dominant epitope peptide of Ebola virus envelope glycoprotein as well as coding gene and application thereof
  • Dominant epitope peptide of Ebola virus envelope glycoprotein as well as coding gene and application thereof
  • Dominant epitope peptide of Ebola virus envelope glycoprotein as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Obtaining the dominant epitope peptide of Ebola virus envelope glycoprotein

[0023] Step1: Amino acid sequence search:

[0024] The glycoprotein of Zaire Ebola virus (GP, accession number: AY354458.1) was obtained from the NCBI GenBank database as input for various bioinformatics tools for epitope prediction, conservation analysis, molecular docking and experiments .

[0025] Step2: Epitope prediction:

[0026] For H2-Db, H2-Dd, H2-Kb, H2-Kd, H2-Kk and H2-Ld epitope prediction, web-based tools such as the IEDB method are recommended (http: / / tools.iedb.org / mhci / ), SMMPMBEC from the immune epitope database (http: / / tools.immunepitope.org / mhci / ), NetMHCpan 4.1 (http: / / www.cbs.dtu.dk / services / NetMHCpan / ), SYFPEITHI (http: / / www.syfpeit hi.de / bin / MHCServer.dll / EpitopePrediction.htm) and Rankpep (http: / / imed.med.ucm.es / Tools / rankpep.html) for prediction. Predicted epitopes that accounted for the top 2% of total predicted epitopes were selected. Finally, epitopes predict...

Embodiment 2

[0051] Application of dominant epitope peptide of Ebola virus envelope glycoprotein

[0052] The invention lays a foundation for the research of the new EBOV genetic engineering vaccine through the prediction of the dominant MHC class I epitope peptide on the EBOV GP, various computer analyzes and ELISpot test verification.

[0053] After the above experimental research, it is found that the dominant MHC class I epitope peptide on EBOV GP can effectively stimulate the body's cellular immune response.

[0054] In recent years, although vaccines related to EBOV have been developed at home and abroad, from the perspective of application, there are still many shortcomings in this type of vaccine, the most important of which is that the cellular immune response stimulated by the body is not good enough, and there are certain defects. security risks. If the EBOV GP dominant MHC class I epitope peptide screened out by the method of the present invention is used to stimulate the body...

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Abstract

The invention belongs to the technical field of microbial immunity, and particularly relates to dominant epitope peptide of Ebola virus envelope glycoprotein as well as a coding gene and application thereof. The epitope peptide is composed of sequences as shown in SEQ ID NO.1-25. According to the dominant epitope peptide of Ebola virus envelope glycoprotein as well as the coding gene and application thereof, potential epitopes derived from EBOV GP are predicted and verified through an ELISpot test, protection properties of the potential epitopes in different isolates around the world are subjected to a variety of deep analyses, so that screened dominant isolates are verified. The binding affinity and immunogenicity of the epitopes and proper sites of respective MHC class I molecules are further predicted, which is finally helpful for understanding the immunobiology of the EBOV GP and perfecting the epitope vaccine design for virus in the future.

Description

technical field [0001] The invention belongs to the technical field of microbial immunity, and in particular relates to a dominant epitope peptide of the envelope glycoprotein of Ebola virus, its coding gene and application. Background technique [0002] Ebola virus disease, caused by the Ebola virus, first appeared in Zaire and adjacent areas of Sudan. The genus Ebolavirus can be further subdivided into five species, named respectively: Zaire ebolavirus (ZEBOV / EBOV), Sudan ebolavirus (Sudan ebolavirus, SUDV), Tai Forest Egypt Bora virus ( Forestebolavirus (TAFV), Bundibugyo ebolavirus (BDBV) and Reston ebolavirus (RESTV). Human-to-human transmission occurs through contact with infected objects and exposure of mucous membranes or skin breaks to infectious body fluids and tissues. Ebola virus has been found in a variety of bodily fluids, including saliva, blood, breast milk, feces, and semen. In addition, there are multiple potential routes of transmission, including dir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/08C12N15/40A61K39/12A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2760/14122C12N2760/14134Y02A50/30
Inventor 杨琨姜东伯刘洋孙报增孙昊孙元杰杨舒雅张溪洋潘婧宇胡尘辰刘天玥王静韩晨颖黄依南丁家琦
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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