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Advanced epitope peptides of hantavirus envelope glycoprotein, and coding gene and application thereof

A technology of enveloped glycoprotein and hantavirus, which is applied in the direction of viral peptides, viruses, applications, etc., to achieve wide application prospects, safe and convenient use

Pending Publication Date: 2022-05-13
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, only peptides with high affinity and tight binding to MHC class I molecules can be expressed on the surface of APC cells and presented to CD8+ T cells for recognition, thereby triggering an immune response

Method used

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  • Advanced epitope peptides of hantavirus envelope glycoprotein, and coding gene and application thereof
  • Advanced epitope peptides of hantavirus envelope glycoprotein, and coding gene and application thereof
  • Advanced epitope peptides of hantavirus envelope glycoprotein, and coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Obtaining the dominant epitope peptide of Hantavirus envelope glycoprotein

[0024] Step1: Amino acid sequence search:

[0025] The glycoprotein sequence (GP, gene number: KT885048.1) of Hantavirus 76-118 was obtained from the NCBI GenBank database as input for various bioinformatics tools for epitope prediction, conservation analysis, molecular docking and multiple Sequence Alignment.

[0026] Step2: Epitope prediction:

[0027] For mouse H2-Db, H2-Dd, H2-Kb, H2-Kd, H2-Kk, and H2-Ld epitope prediction, web-based tools such as the IEDB method are recommended (http: / / tools.iedb.org / mhci / ), the SMMPMBEC method (http: / / tools.immunepitope.org / mhci / ), the NetMHCpan4.1 method (http: / / www.cbs.dtu.dk / services / NetMHCpan / ) from the immune epitope database, The SYFPEITH method (http: / / www.syfpeithi.de / bin / MHCServer.dll / EpitopePrediction.htm) and the Rankpep method (http: / / imed.med.ucm.es / Tools / rankpep.html) were used for prediction. Predicted epitopes that accounte...

Embodiment 2

[0053] Example 2: Application of the dominant epitope peptide of Hantavirus envelope glycoprotein

[0054] The invention lays a foundation for the research of the HTNV novel genetic engineering vaccine through the prediction of the dominant MHC I epitope peptide on the HTNV GP and various computer analyzes.

[0055] Step1: Peptide-MHC molecular docking

[0056] HPEPDOCK is a new type of web server, which can simulate molecular docking by inputting 9-peptide dominant epitope sequence and MHC class I molecule RDB format file, so as to obtain the docking model. Use the RCSB PDB database (https: / / www.rcsb.org / ) to obtain relevant MHC class I molecular 3D structure data {HLA-A1[HLA-A*0206(3OXR)], HLA-B7[HLA-B* 0702(5EO1), HLA-B*3501(1A9E), HLA-B*5101(1E28), HLA-B*5301(1A1N)], HLA-B8[HLA-B*0801(4QRP)], HLA-B15 [HLA-B*1501(1XR9), HLA-A*0101(4NQV)], HLA-B44[HLA-B*4402(3KPL)], H2-Ld(6L9M), H2-Kb(6JQ3), H2- Db(1JUF)}. The docking of each 9-peptide with the same MHC class I molecule ...

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Abstract

The invention belongs to the technical field of microbial immunity, and particularly relates to dominant epitope peptides of hantavirus envelope glycoprotein as well as a coding gene and application of the dominant epitope peptides. The epitope peptide is composed of sequences as shown in SEQ ID NO.1-11. The invention further discloses a preparation method of the epitope peptide. According to the invention, 11 candidate epitopes are obtained by screening and verification through a method for predicting and identifying antigen-specific optimized epitopes of HTNV GP, and then verification is carried out by simulating molecular docking. The immunoreactivity of the epitope in different isolates around the world is deeply compared and analyzed to verify the stability of the epitope in vaccine application. In a word, the generic MHC-I immunoreactivity of the HTNV GP is comprehensively evaluated according to comparative immunology, so that the immunobiology of the HTNV GP can be understood, and guidance is provided for development of HTNV epitope vaccines in the future.

Description

technical field [0001] The invention belongs to the technical field of microbial immunity, and in particular relates to a group of dominant epitope peptides of hantavirus envelope glycoproteins, encoding genes and applications thereof. Background technique [0002] Hantavirus, belonging to the Bunyaviridae family, is an enveloped, segmented, negative-strand RNA virus that causes two zoonotic diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantavirus (HTNV) is the main pathogen causing severe HFRS. At present, the disease occurs in more than 70 countries around the world, with about 20,000-50,000 new cases every year, and the fatality rate is about 1%-15%. Therefore, the high prevalence of HFRS is still considered a serious public health problem. [0003] The HTNV genome consists of three segments, S, M, and L, which encode nucleocapsid protein (NP), envelope glycoprotein (GP) and RNA-dependent RNA polymerase (RdRp), respecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/175C12N15/40A61K39/12A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2760/12122C12N2760/12134C12N2760/12133
Inventor 杨琨姜东伯孙报增张俊琦刘洋王佳伟孙昊张佳幸杨舒雅孙元杰胡尘辰刘天玥王静乔旭鹏陈佳希
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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