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Culture medium, bacillus subtilis and composite strain

A Bacillus subtilis, culture medium technology, applied in the directions of bacteria, fungi, microorganism-based methods, etc., can solve the problems of poor palatability, restricted use, low protein content, etc., and achieves low crude fiber output, low price, and promotion. effect of proliferation

Pending Publication Date: 2021-11-09
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition to being rich in soluble polysaccharides, vitamins, crude fat, mineral elements and other nutrients, as well as polyphenols and flavonoids functional substances, citrus pomace also contains a lot of water and cellulose, and the protein content is not high, palatable Feed it directly to animals, which can affect its palatability and digestibility, is not conducive to the digestion and absorption of animals, and then affects the growth performance of animals, seriously restricting its use as feed directly, resulting in the disposal of citrus residue waste increasing costs

Method used

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  • Culture medium, bacillus subtilis and composite strain
  • Culture medium, bacillus subtilis and composite strain
  • Culture medium, bacillus subtilis and composite strain

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Embodiment 1

[0036] Raw material source:

[0037] Bacillus subtilis: kept in this laboratory

[0038] Aspergillus niger: Presented by the Fruit and Vegetable Deep Processing Laboratory of Sericulture and Agricultural Products Processing Institute, Guangdong Academy of Agricultural Sciences

[0039] Candida tropicalis: a gift from the Fruit and Vegetable Deep Processing Laboratory of the Sericulture and Agricultural Products Processing Institute of Guangdong Academy of Agricultural Sciences

[0040] Lactobacillus plantarum: preserved in this laboratory

[0041] Part I Carbon Source Optimization

[0042] Glucose, lactose, bran, soluble starch, corn flour, and molasses were used to replace the carbon source in the LB liquid basal medium, and the content was 0.5%. LB liquid basal medium adopts commercially purchased LB basal medium, mainly composed of 1% tryptone, 0.5% yeast extract and 1% NaCl. Make up to 1 L with distilled water, and sterilize under high temperature and high pressure at ...

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Abstract

The invention belongs to the technical field of biological fermentation, and discloses a culture medium for optimizing the activity of CMCA and FPA enzymes of bacillus subtilis. The culture medium is prepared from the following components: 9-11g / L of sucrose, 4.5-5.5 g / L peptone, 4.5-5.5 g / L of yeast extract, 2.5-3.5 g / L of MgSO4.7H2O, 1.5-2 g / L of FeSO4.7H2O, 0.1-0.5 g / L of sodium selenite and 0.1-0.3 g / L of manganese sulfate. The culture medium can optimize the enzyme activity values of CMCA and FPA of bacillus subtilis. The invention further discloses bacillus subtilis which is cultured by the culture medium and has excellent enzyme activity values of CMCA and FPA. Finally, the invention further discloses a composite strain.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium, Bacillus subtilis and composite strains. Background technique [0002] China is a big country of fruits. In addition to most of them being used for fresh food, a large part is used for juicing and canning, which in turn produces a large amount of by-products such as skin dregs. According to statistics, my country produces more than 40 million tons of pomace annually, of which more than 5 million tons of citrus pomace. In addition to being rich in soluble polysaccharides, vitamins, crude fat, mineral elements and other nutrients, as well as polyphenols and flavonoids functional substances, citrus pomace also contains a lot of water and cellulose, and the protein content is not high, palatable Feed it directly to animals, which can affect its palatability and digestibility, is not conducive to the digestion and absorption of animals, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12N1/16C12N1/14C12R1/125C12R1/685C12R1/74
CPCC12N1/20C12N1/38C12N1/16C12N1/14
Inventor 余苗马现永容庭李贞明刘志昌崔艺燕田志梅邓盾
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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