Breathable membrane for culturing high-yield varicella virus and culture method of high-yield varicella virus

A technology of varicella virus and culture method, which is applied in the direction of virus, virus/bacteriophage, double-stranded DNA virus, etc. It can solve the problems of not having oxygen selectivity, affecting the proliferation of varicella virus, affecting oxygen supply, etc., and achieving high-yield culture , enhance cell adhesion, enhance the effect of proliferation and differentiation

Active Publication Date: 2021-11-09
江苏金迪克生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing gas-permeable membranes are usually fluorinated ethylene propylene membranes, polycarbonate membranes, and polystyrene membranes. These membranes are usually

Method used

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  • Breathable membrane for culturing high-yield varicella virus and culture method of high-yield varicella virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] S1: Dissolve polypropylene and polycaprolactone in DMF-dichloromethane mixed solvent as a spinning solution, set the conditions: temperature is 25°C, relative humidity is 50%, feeding speed is 0.5mL / h, receiving distance 15cm, voltage 13Kv, electrospinning to obtain a fiber membrane; place it in a 0.4mol / L arginine aqueous solution, set the temperature at 40°C, shake for 2 hours, wash with water, and obtain a polycaprolactone composite membrane ,spare;

[0037] S2: (1) Set the temperature at 0°C, add the sodium nitrite solution to the pyridine solution and mix, add the multi-walled carbon nanotube dispersion, and stir for 2.5 hours; set the temperature at 26°C, stir for 9 hours, and wash until Neutral, dry; under inert gas, ultrasonically disperse it in the solvent, add cobalt phthalocyanine, set the temperature at 72°C and reflux for 2.3 hours, wash and filter; disperse it and polypropylene pyrrolidone in DMF-water, ultrasonic for 7 hours, Washing and drying to obtain...

Embodiment 2

[0041] S1: Dissolve polypropylene and polycaprolactone in DMF-dichloromethane mixed solvent as a spinning solution, set the conditions: temperature is 23°C, relative humidity is 48%, feeding speed is 0.4mL / h, receiving distance 15cm, voltage 12Kv, electrospinning to obtain a fiber membrane; place it in 0.1mol / L arginine aqueous solution, set the temperature at 28-42°C, shake for 1 hour, wash with water to obtain polycaprolactone Composite film, spare;

[0042] S2: (1) Set the temperature at 0°C, add the sodium nitrite solution to the pyridine solution and mix, add the multi-walled carbon nanotube dispersion, and stir for 2 hours; set the temperature at 25°C, stir for 8 hours, and wash until Neutral, dry; under inert gas, ultrasonically disperse it in the solvent, add cobalt phthalocyanine, set the temperature at 70°C to reflux for 2 hours, wash and filter; disperse it and polypropylene pyrrolidone in DMF-water, ultrasonic for 6 hours, Washing and drying to obtain cobalt phtha...

Embodiment 3

[0046] S1: Dissolve polypropylene and polycaprolactone in a mixed solvent of DMF-dichloromethane as a spinning solution, setting conditions: temperature 28°C, relative humidity 52%, feeding speed 0.6mL / h, receiving distance 15cm, voltage 15Kv, electrospinning to obtain a fiber membrane; place it in a 0.5mol / L arginine aqueous solution, set the temperature at 42°C, shake for 2 hours, wash with water, and obtain a polycaprolactone composite membrane ,spare;

[0047] S2: (1) Set the temperature at 3°C, add the sodium nitrite solution to the pyridine solution and mix, add the multi-walled carbon nanotube dispersion, and stir for 3 hours; set the temperature at 28°C, stir for 10 hours, and wash until Neutral, dry; under inert gas, ultrasonically disperse it in the solvent, add cobalt phthalocyanine, set the temperature at 75°C to reflux for 3 hours, wash and filter; disperse it and polypropylene pyrrolidone in DMF-water, ultrasonically for 8 hours, Washing and drying to obtain cob...

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Abstract

The invention discloses a breathable membrane for culturing a high-yield varicella virus and a culture method of the high-yield varicella virus. The breathable membrane is applied to a culture room for culturing the high-yield varicella virus. The breathable membrane sequentially comprises a breathable layer and a cell adhesion layer; the breathable layer is a polydimethylsiloxane composite membrane; and the cell adhesion layer is a polycaprolactone composite membrane. The breathable membrane and the culture method have the beneficial effects that (1) the polydimethylsiloxane composite membrane capable of enhancing oxygen permeability is prepared through regulation and control to provide oxygen for culture of varicella viruses, and the problem of gas exchange of carbon dioxide and oxygen is effectively solved in cooperation with a carbon dioxide incubator; (2) the polycaprolactone composite membrane capable of enhancing cell adhesion is prepared through regulation and control to enhance the adhesion and diffusion of the varicella viruses; and (3) the polydimethylsiloxane composite membrane and the polycaprolactone composite membrane are compounded by using diluted polydimethylsiloxane to form a breathable membrane with both breathability and cell adhesion, and the proliferation and differentiation of the varicella viruses are synergistically enhanced to realize high-yield culture.

Description

technical field [0001] The invention relates to the technical field of varicella virus culture, in particular to a gas permeable membrane for high-yield varicella virus culture and a culture method thereof. Background technique [0002] Chickenpox is an infectious disease caused by shingles virus infection, so it is necessary to cultivate varicella virus to prepare a vaccine to prevent chickenpox. Among them, varicella virus culture is indispensable to oxygen. In common incubators, oxygen usually comes from the single-way diffusion movement from the top air-liquid interface to the bottom, while varicella virus culture is generally static culture without medium flow. Therefore, during the culture process There is a high cell density of varicella virus in the medium. When the dissolved oxygen reaches the bottom, the oxygen is almost exhausted, which makes the quality of varicella virus uneven. The way to solve the problem is to use a gas-permeable membrane to provide oxygen t...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12N7/00
CPCC12M23/24C12M25/02C12N7/00C12N2710/16752Y02A50/30
Inventor 杨骏宇余军张笑雨唐阳展静欧智奇望朔杨文彬朱实惠吴建华付建林
Owner 江苏金迪克生物技术股份有限公司
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