Reusable optical biosensor chip and method for realizing reuse of optical biosensor chip

Abstract

The invention discloses a reusable optical biosensor chip and a method for realizing reuse of the optical biosensor chip, and relates to the field of biosensing. A parylene C film is attached to the surface of a nano structure of the optical biosensor chip, and the parylene C film is coupled with an antibody and an antigen; and the nanostructure of the optical biosensor chip is coupled with the antibody and the antigen thereof by using parylene C, and parylene C is removed after the detection is completed. The parylene C film is used as a coupling bridge between the optical biosensor chip nanostructure and the specific antibody, a specific parylene C growth thickness and a removal method are adopted, the surface property of the nanostructure is not influenced, parylene C can be grown on the chip again, and a spectral result shows that the spectrum of the chip after parylene C is removed completely coincides with that of an initial chip.

Description

technical field [0001] The invention relates to the technical field of biosensing, in particular to a reusable optical biosensor chip and a method for realizing the repeated use of the optical biosensor chip. Background technique [0002] With the development of micro-nano processing technology, optical biosensors, especially plasmon-based biosensors, have aroused great interest of researchers due to their advantages of high sensitivity, rapidity, and label-free detection, and are widely used in the detection of various biomarkers. Sensing detection. In addition, these sensor chips can be fabricated in a small area using CMOS technology, making it possible to achieve high integration of optical biosensing structures and microfluidic chips. [0003] The core "hot spot" structure of the optical biosensing chip is composed of various fine nanostructures. High-sensitivity nanostructures are prepared by electron beam lithography and ion beam etching techniques, and combined with...

AI Technical Summary

Problems solved by technology

At present, the methods of immobilizing antibodies are divided into covalent coupling and physical adsorption. Covalent coupling usually uses multi-level compounds to connect through chemical bonds. The temperature and pH of the buffer will affect the modification efficiency, and the steps are cumbersome. Gold, silicon and other nanostructures are non-specifically adsorbed by labeled BSA, and then coupled with a specific antibody labeled with a label molecule. Once the antibody is successfully modified, it cannot be removed, and the chip can only be used once.

At present, the method that can realize the reusability of the chip is mainly to break the chemical bond between the chip and the modifier through chemical reagents, but the stability of the chip is poor, and the effect of reusing is not ideal.

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