Preparation method of marine plant polysaccharide anti-adhesion material

A marine plant and anti-adhesion technology, which is applied in drug delivery, medical formula, surgery, etc., can solve the problems that sodium hyaluronate is easy to cause fever, polylactic acid degrades for a long time, and the human body is easy to cause inflammation, etc., so as to prevent adhesion and facilitate Manipulate, reduce the effect of manipulating

Active Publication Date: 2021-11-02
QINGDAO CHENLAND MARINE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the anti-adhesion products on the market mainly include sodium hyaluronate, chitosan anti-adhesion film, polylactic acid, etc. Sodium hyaluronate is easy to cause fever symptoms, and the degradation time in the body is too short to effectively prevent tissue adhesion; After using chitosan for anti-adhesion, patients will feel discomfort and feel foreign objects in the surgical site, and chitosan is an animal-derived material, which has the risk of immunogenicity and virus transmission; polylactic acid degrades for a long time and exists in the The human body is prone to inflammation, and lactic acid, one of the degradation products, will stimulate the body; in addition, the above products need to be used after complete hemostasis

Method used

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  • Preparation method of marine plant polysaccharide anti-adhesion material
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  • Preparation method of marine plant polysaccharide anti-adhesion material

Examples

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Embodiment 1

[0026] A preparation method of marine plant polysaccharide anti-adhesion material, the specific steps are as follows:

[0027] (1) Dissolve commercially available pharmaceutical excipient grade sodium alginate with a molecular weight of 10,000 in 0.4% w / w sodium chloride aqueous solution to make a solution with a concentration of 2% w / w; add activated carbon, activated carbon and alginic acid The mass ratio of sodium is 3:1, stir at 30°C for 4 hours, centrifuge the mixture at 9000 rpm for 30 minutes, and take the supernatant to obtain sodium alginate solution;

[0028] (2) With stirring at 50°C and 500rpm, add 1% w / w calcium chloride to the sodium alginate solution obtained in step (1) through a 26G injection needle to form gel microspheres, continue to stir for 2 hours, and centrifuge at 9000rpm After 30 minutes, take the precipitate and wash the gel microspheres fully with distilled water;

[0029] (3) Stir and soak the gel microspheres cleaned in step (2) in a lactic acid ...

Embodiment 2

[0034] A preparation method of marine plant polysaccharide anti-adhesion material, the specific steps are as follows:

[0035] (1) Dissolve commercially available pharmaceutical excipient grade potassium alginate with a molecular weight of 30,000 in 0.6% w / w sodium nitrate aqueous solution to make a solution with a concentration of 2% w / w; add activated carbon, activated carbon and potassium alginate The mass ratio of the mixture is 3:1, stirred at 40°C for 3 hours, centrifuged at 10,000 rpm for 20 minutes, and the supernatant was taken to obtain a potassium alginate solution;

[0036] (2) Under stirring at 50°C and 1000rpm, add 5% w / w calcium gluconate through a 20G injection needle into the potassium alginate solution obtained in step (1) to form gel microspheres, and continue stirring for 1.5h at 10000rpm Centrifuge for 20 minutes, take the precipitate and wash the gel microspheres fully with distilled water;

[0037] (3) Stir and soak the gel microspheres cleaned in step ...

Embodiment 3

[0042] A preparation method of marine plant polysaccharide anti-adhesion material, the specific steps are as follows:

[0043] (1) Dissolve oxidized sodium alginate with a molecular weight of 100,000 in 0.9% w / w sodium lactate aqueous solution to make a solution with a concentration of 2% w / w; add activated carbon, the mass ratio of activated carbon to oxidized sodium alginate is 3 : 1. Stir at 50°C for 0.5h, centrifuge the mixed solution at 12000rpm for 15min, take the supernatant to obtain an oxidized sodium alginate solution;

[0044] (2) Add 10% w / w calcium nitrate to the oxidized sodium alginate solution obtained in step (1) through a 24G injection needle under stirring at 30°C at 1500rpm to form gel microspheres, continue stirring for 1h, and centrifuge at 12000rpm After 15 minutes, take the precipitate and wash the gel microspheres fully with distilled water;

[0045] (3) Stir and soak the gel microspheres cleaned in step (2) in a lactic acid solution of pH=4 for 10 hour...

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Abstract

The invention discloses a preparation method of a marine plant polysaccharide anti-adhesion material, and belongs to the technical field of biomedical materials. The invention discloses a preparation method of a marine plant polysaccharide anti-adhesion material, which comprises the following steps: by taking pharmaceutic adjuvant-grade alginate as a material, performing activated carbon adsorption and centrifugation, performing calcium ion crosslinking on supernate to form gel microspheres, soaking the gel microspheres in an acid solution and an alkaline solution, and performing spray drying to obtain powder, finally, filling and sterilizing to obtain the marine plant polysaccharide anti-adhesion material. The marine plant polysaccharide anti-adhesion material is simple and safe, the preparation process is simple and easy to implement, the preparation time is short, the biocompatibility is good, and gel hemostasis adhesion can be formed after liquid absorption; the degradation time can be consistent with the adhesion formation critical period, adhesion formation is prevented in the whole process, and the overall anti-adhesion effect is achieved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a preparation method of a marine plant polysaccharide anti-adhesion material. Background technique [0002] After surgery, tissue adhesion is prone to occur, which is not only a common clinical phenomenon in the field of surgery, but also a process that patients must go through during the healing process. Adhesions are abnormal structures where fibrous bands of connective tissue join together with adjacent tissues or organs. Adhesions can range in size from a thin film to a dense vascular scar. Postoperative tissue adhesions occur in almost any body part that is surgically treated, but are particularly common after procedures such as the abdomen and pelvis, tendons, and spine. It is reported that 50%-100% of abdominal and pelvic surgery can lead to different degrees of adhesions. If the adhesion phenomenon occurs in the abdominal cavity, pelvic cavity, bone and ot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/02A61L31/14A61L31/16
CPCA61L31/042A61L31/024A61L31/028A61L31/14A61L31/148A61L31/146A61L31/16A61L2400/06A61L2400/04A61L2300/412A61L2300/232C08L5/04
Inventor 王宝群刘桂亭邹圣灿林莎莎
Owner QINGDAO CHENLAND MARINE BIOTECH CO LTD
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