Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Research on influence of emodin on physiological indexes of megalobrama amblycephala infected with aeromonas hydrophila

A technology of Aeromonas hydrophila and physiological indicators, applied in the field of freshwater fishery aquaculture, can solve the problems of high mortality of fish and little research on antibacterial ability, so as to improve the infection ability, promote the secretion of cytokines, and improve the body The effect of phagocytosis

Pending Publication Date: 2021-11-02
FRESHWATER FISHERIES RES INST OF SHANDONG PROVINCE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its breeding environment contains a large number of bacteria, viruses and exogenous pathogenic factors, etc. (Li et al., 1993), which lead to large-scale outbreaks of diseases of bream, resulting in high mortality of fish
Preliminary experiments have proved that emodin can be used as an immune enhancer to promote the growth, non-specific immunity and anti-Aeromonas hydrophila ability of bream, but emodin is antibacterial to bream infected with Aeromonas hydrophila. little research has been done on the effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Research on influence of emodin on physiological indexes of megalobrama amblycephala infected with aeromonas hydrophila
  • Research on influence of emodin on physiological indexes of megalobrama amblycephala infected with aeromonas hydrophila
  • Research on influence of emodin on physiological indexes of megalobrama amblycephala infected with aeromonas hydrophila

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] Example 1 Effect of Emodin on the Total Bacterial Count of Aeromonas in Each Tissue of Megalobrama after Intraperitoneal Injection of Aeromonas hydrophila

[0120] 1.1 Count of Aeromonas bacteria in the blood and tissues of bream

[0121] Under aseptic conditions, take the blood, kidney and liver tissue of bream, thaw the kidney and liver tissue at room temperature and weigh them, add sterile saline at a ratio of 1:9, and homogenate with 10ml of sterilized glass The blood dilution and the homogenized liver and kidney bacterial suspension were used as the original solution, and the blood, liver and kidney bacterial suspension were used as the original solution, and the samples were diluted 10 times by 10 times dilution method. -1 , 10 -2 、10 -3 and 10 -4 A total of 4 gradients, 0.1mL of the stock solution and samples of each dilution were spread on the RS selective medium plate, and the plates were placed in a constant temperature incubator for 20 hours of overnight c...

Embodiment 2

[0130] Example 2 Study on the Effect of Emodin on the Immune Index of Brachycephalus after Intraperitoneal Injection of Aeromonas hydrophila

[0131] 2.1.1 White blood cell count and respiratory burst activity determination

[0132] 50 μL of anticoagulated fresh blood drawn during sampling was used for white blood cell (WBC) count, and the white blood cell count was measured on an automatic blood cell analyzer (Mindray BC-5300Vet), and the reagents used were provided by Shenzhen Mindray Biomedical Electronics Co., Ltd.

[0133] Respiratory burst activity was modified according to the descriptions of Secombes et al. (1990), Song et al. (1994) and Ai et al. (2007), and was determined by the NBT method. Take 100 μL of anticoagulated fresh blood drawn during sampling, and add 100 μL of 0. 2% NBT (Nitroblue tetrazolium, Sigma), incubate at 37°C for 30min, take 15μL of the reaction solution, add 300μL of N,N-dimethylformamide (DMF), mix well, and centrifuge at 2000r / min, 4°C for 5mi...

Embodiment 3

[0148] Example 3 Study on the Effect of Emodin on the Antioxidant Index of Brachycephalus after Intraperitoneal Injection of Aeromonas hydrophila

[0149] 3.1 Plasma SOD activity and MDA content

[0150] Determination of SOD activity According to (Granelli et al., 1995) report, the active oxygen anion produced by the oxidation of xanthine MDA content was determined according to (Drape et al., 1993) report, using thiobarbituric acid colorimetric assay, SOD activity and MDA content assay kits were purchased from Nanjing Jiancheng Biological Engineering Co., Ltd. graduate School.

[0151] 3.2 Result Analysis

[0152] During the 14-day test period, the plasma SOD activity of the group head bream in the negative control group, positive control group, 30, 100, and 150mg / kg emodin group was as follows: Figure 4 As shown in A. The results showed that at the beginning of the experiment and at 1, 7, and 14 days of feeding, compared with the negative control group, there was no sig...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a research on influence of emodin on physiological indexes of megalobrama amblycephala infected with aeromonas hydrophila. The research comprises the following steps: researching the influence of emodin on the total bacterial count of aeromonas in each tissue of megalobrama amblycephala after intraperitoneal injection of aeromonas hydrophila, researching the influence of emodin on the immune index of megalobrama amblycephala after intraperitoneal injection of aeromonas hydrophila, and researching the influence of emodin on the liver tissue structure of megalobrama amblycephala after intraperitoneal injection of aeromonas hydrophila. The invention provides research on the influence of emodin on the anti-oxidation index of megalobrama amblycephala after intraperitoneal injection of aeromonas hydrophila and research on the influence of emodin on the relative expression quantity of Cu / Zn-SOD, CAT, GPx, TNF-alpha and IL-1 beta mRNA of megalobrama amblycephala after intraperitoneal injection of aeromonas hydrophila. Results show that by adding 100mg / kg of emodin into daily ration, the in-vivo pathogenic bacteria can be killed and the aeromonas hydrophila infection resistance of megalobrama amblycephala can be improved by improving the phagocytic function of the body, promoting the secretion of cell factors and the like, so that the immune function of the body can be recovered.

Description

technical field [0001] The present invention relates to a study on the influence of Chinese herbal medicine on the ability of freshwater fish to resist the infection of Aeromonas hydrophila, specifically a study on the influence of emodin on the physiological indicators of bream infected with Aeromonas hydrophila, which belongs to freshwater fishery Farming application field. Background technique [0002] Aeromonas hydrophila (Aeromonas hydrophila) widely exists in nature, commonly found in fresh water, sewage, sludge, soil and human feces (Zhai Guocai, 1997). It has a wide range of pathogenicity, and can infect various animals including cold-blooded animals such as fish, amphibians, reptiles, poultry and mammals, leading to local infections such as sepsis or skin ulcers. In addition, it can also cause large-scale disease outbreaks in aquatic animals, especially fish, in hot summer (Larsen et al., 1976). Once the fish is diseased, it can lead to high mortality and cause in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/122A61K36/704A61P31/04A61P39/06A61K49/00A01K61/10G01N33/68G01N33/74G01N33/78C12Q1/6851C12Q1/54A61K125/00
CPCA61K31/122A61K36/704A61P31/04A61P39/06A61K49/0008A01K61/10G01N33/6869G01N33/6863G01N33/78G01N33/743C12Q1/6851C12Q1/54G01N2333/525G01N2333/545C12Q2531/113C12Q2563/107C12Q2545/113Y02A40/81
Inventor 张媛媛王晓丽朱永安刘亚
Owner FRESHWATER FISHERIES RES INST OF SHANDONG PROVINCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com