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Bionic nano material as well as preparation method and application thereof

A technology of biomimetic nano and nano materials, applied in the field of medicine, can solve problems such as difficulties in large-scale production, weak surface modification ability, and restrictions on biological applications, and achieve the effects of improving biological safety, reducing damage, and prolonging cycle time

Pending Publication Date: 2021-10-22
CHANGSHA WANOU CHEM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of oxygen production, which can continuously catalyze and not consume itself, is expected to develop a persistent and stable nanoenzyme active preparation for tumor treatment, but the material toxicity and weak surface modification ability of ceria limit the The biological application of this material, in addition, most of the synthesis methods of ceria require extremely high calcination temperature, which also makes it difficult for large-scale production. Therefore, it is necessary to develop a safer, reliable and easy-to-synthesize ceria nano-enzyme preparation

Method used

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  • Bionic nano material as well as preparation method and application thereof
  • Bionic nano material as well as preparation method and application thereof
  • Bionic nano material as well as preparation method and application thereof

Examples

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Embodiment 1

[0036] A kind of biomimetic nano material provided by the present invention, biomimetic nano material comprises cerium oxide, polyacrylic acid, chlorin e6, cinobufaction base and biomimetic membrane, polyacrylic acid is coated on the surface of cerium oxide to synthesize nano material, described Cinobufacin and chlorin e6 were respectively loaded on the surface of nanomaterials through physical adsorption and chemical bonding to obtain nanocomposites. The biomimetic film was coated on the outer layer of nanocomposites to obtain biomimetic nanomaterials. The particle size of biomimetic nanomaterials was 10nm-20nm.

[0037] The invention provides a method for preparing the above-mentioned biomimetic nanomaterial, comprising the following steps:

[0038] S1, 1M cerium ion (this method utilizes concentrated nitric acid to decompose 2.3g cerium carbonate to obtain) and 1g polyacrylic acid are evenly dispersed in double distilled water, slowly add dropwise 40ml 30% ammonia water and...

Embodiment 2

[0047] Application of a polyacrylic acid-modified ceria biomimetic nanomaterial co-loaded with chlorin e6 and cinobufaction group of the present invention in immune evasion and tumor targeting, using the co-loaded chlorin prepared in Example 1 Polyacrylic acid-modified ceria biomimetic nanomaterials based on phene e6 and cinobufin.

[0048] The polyacrylic acid-modified cerium oxide (CPCC) co-loaded with chlorin e6 and cinobufaction group and the polyacrylic acid-modified ceria biomimetic nanomaterial (CPCCM) with co-loaded chlorin e6 and cinobufaction group ) were co-incubated with macrophages to observe the degree of uptake, in which the gray fluorescence represents the nanocomposite material (CPCC) / nano biomimetic material (CPCCM) entering the macrophage, the results are as follows Figure 5 As shown, the nanocomposite without biomimetic membrane (CPCC) was recognized and phagocytized by macrophages when the concentration reached above 100 μg / ml, while the nanocomposite wit...

Embodiment 3

[0050] Application of a polyacrylic acid-modified ceria biomimetic nanomaterial co-loaded with chlorin e6 and cinobufaction group of the present invention in anti-breast cancer cells in vitro, using the co-loaded chlorin prepared in Example 1 Polyacrylic acid-modified ceria biomimetic nanomaterials based on phene e6 and cinobufin.

[0051] The activity of laser-activated / deactivated chlorin e6 (Ce6), co-loaded chlorin e6 and cinobufinin under hypoxic environment was evaluated by live-death staining (gray dots in the figure are labeled dead cells) The killing effect of polyacrylic acid-modified ceria (CPCC) and polyacrylic acid-modified ceria biomimetic nanomaterials (CPCCM) co-loaded with chlorin e6 and cinobufaction group (CPCCM) on breast cancer cells. Such as Figure 7 As shown, due to the influence of hypoxic environment, chlorin e6 cannot exert the normal photodynamic killing effect under laser irradiation, and the fluorescence intensity is weak. The fluorescence of CPC...

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Abstract

The invention discloses a bionic nano material as well as a preparation method and application thereof. The invention relates to a biomimetic nano material which comprises cerium dioxide, polyacrylic acid, chlorin e6, cinobufagin and a biomimetic membrane, the polyacrylic acid is coated on the surface of the cerium dioxide to synthesize the nano material, the cinobufagin and the chlorin e6 are loaded on the surface of the nano material through physical adsorption and chemical bond combination to obtain a nano composite material, the outer layer of the nano composite material is coated with the biomimetic membrane to obtain the biomimetic nano material, and the invention further provides a preparation method which is easy to operate and easy for large-scale production of the biomimetic nano material. The biomimetic nanomaterial disclosed by the invention has relatively good stability, dispersity and safety, is long in oxygen production strategy duration time, can still keep an effective photodynamic killing effect in an oxygen-deficient environment, is beneficial to reducing side effects of chemotherapeutic drugs and enhancing tumor enrichment efficiency of the chemotherapeutic drugs, and is applied to anti-tumor treatment of photodynamic therapy combined with chemotherapy.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a biomimetic nano material and its preparation method and application. Background technique [0002] Chemotherapy is one of the most widely used tumor treatment methods at present, but the systemic distribution and lack of targeting ability of chemotherapeutic drugs also lead to damage to healthy cells. With the development of nanotechnology in medical applications, combination therapy brings new hope to this dilemma by combining multiple treatment modalities to reduce the amount of chemotherapy drugs or improving drug targeting strategies through drug delivery systems. The combination of photodynamic / chemotherapy has developed a variety of excellent therapeutic strategies due to its good regioselectivity, minimal invasiveness, and low toxicity. However, photodynamic therapy, as an oxygen-dependent treatment method, is incompatible with the hypoxic environment inside the tumor ...

Claims

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Application Information

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IPC IPC(8): A61K47/58A61K9/14A61K47/02A61K47/32A61K41/00A61P35/00A61K31/585A61K33/244
CPCA61K47/58A61K41/0071A61K33/244A61K31/585A61K9/143A61K9/146A61P35/00A61K2300/00
Inventor 欧青
Owner CHANGSHA WANOU CHEM TECH CO LTD
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