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Preparation method and application of bone black/CMC (carboxy methylated cellulose) stabilized FeS composite material loaded phosphate solubilizing bacteria functionalized microbial agent

A technology of composite materials and phosphate-dissolving bacteria, applied in the field of soil remediation, can solve the problems of low passivation efficiency, achieve the effect of enhancing passivation, enhancing biological functions, and promoting development and application

Pending Publication Date: 2021-10-15
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low passivation efficiency due to the high concentration of lead will seriously inhibit the biological activity of phosphate-solubilizing bacteria (PSB) when using bioremediation to repair lead pollution, and provide bone char / CMC stabilized FeS composite Preparation method and application of material-loaded functional bacterial agent of phosphate-solubilizing bacteria

Method used

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  • Preparation method and application of bone black/CMC (carboxy methylated cellulose) stabilized FeS composite material loaded phosphate solubilizing bacteria functionalized microbial agent
  • Preparation method and application of bone black/CMC (carboxy methylated cellulose) stabilized FeS composite material loaded phosphate solubilizing bacteria functionalized microbial agent
  • Preparation method and application of bone black/CMC (carboxy methylated cellulose) stabilized FeS composite material loaded phosphate solubilizing bacteria functionalized microbial agent

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specific Embodiment approach 1

[0020] Specific embodiment one: the preparation method of the functional bacterial agent of bone charcoal / CMC stabilized FeS composite material loaded phospholytic bacteria in this embodiment is carried out according to the following steps:

[0021] 1. Add bovine bone powder into the tube furnace, heat it to 490-510°C under nitrogen atmosphere, and keep it warm at 490-510°C for 110-120 minutes. After the heat preservation is over, cool it down, rinse it after soaking, and dry it overnight. , to get the original bone char; the FeSO 4 ·7H 2 O was added to deionized water, under nitrogen atmosphere, carboxymethyl cellulose solution was added, and magnetically stirred for 0.5-1h to obtain Fe(II)-CMC complex, the FeSO 4 ·7H 2The ratio of the mass of O to the volume of the carboxymethyl cellulose solution is (2.5-2.7) g: (15.5-17.5) mL, and the mass fraction of carboxymethyl cellulose in the carboxymethyl cellulose solution is 4-5%; Add raw bone charcoal to the Fe(II)-CMC complex...

specific Embodiment approach 2

[0026] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the bovine bone powder described in step 1 is prepared according to the following steps: after cleaning the bovine bone for 3 to 5 times, place it in an oven and dry it at 80 to 90°C Dry for 12-24 hours, pulverize, and pass through a 100-mesh sieve to obtain bovine bone powder.

[0027] Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0028] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is: in step 1, add bovine bone powder into the tube furnace, and heat it to 490-510°C at a heating rate of 10°C / min under a nitrogen atmosphere. .

[0029] Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a preparation method and application of a bone black / CMC stabilized FeS composite material loaded phosphate solubilizing bacteria functionalized microbial agent, and relates to the technical field of soil remediation. The invention aims to solve the problem of low passivation efficiency caused by serious inhibition of biological activity of phosphate solubilizing bacteria (PSB) by high-concentration lead when lead pollution is repaired by adopting a bioremediation method. The method comprises the following steps: placing phosphate solubilizing bacteria in an LB culture medium, and carrying out shake culture for 7-9 hours to obtain a phosphate solubilizing bacteria suspension; and mixing the bone black / CMC stabilized FeS composite material with a phosphate solubilizing bacteria suspension, carrying out shake culture for 5-8 hours, centrifuging, washing, and freeze-drying to obtain the bone black / CMC stabilized FeS composite material loaded phosphate solubilizing bacteria functionalized microbial agent. The invention discloses a preparation method and application of the bone black / CMC stabilized FeS composite material loaded phosphate solubilizing bacteria functional microbial agent.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a preparation method and application of a functional bacterial agent loaded with phosphate-dissolving bacteria on a bone char / CMC stabilized FeS composite material. Background technique [0002] Lead is one of the most frequently detected heavy metals in soil and can accumulate in soil through pesticide application, solid waste disposal, atmospheric deposition, and wastewater irrigation. After entering the soil, lead is difficult to degrade, migrate and accumulate easily, which can have harmful effects on soil microbial species and community structure, leading to a decline in soil fertility, interfering with the normal growth of crops, and causing crop yield reduction. In addition, lead pollutants can also accumulate in animals and humans through the conduction of the food chain, posing a serious threat to the ecological environment, food safety and human health. It can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C12N11/14C12N1/20B09C1/10
CPCC12N11/12C12N11/14C12N1/20B09C1/10
Inventor 张颖曲建华魏书奇孟庆娟刘洋王思琪毕馥漩
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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