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Pseudomonas nitroreducens, microbial inoculum, application of Pseudomonas nitroreducens and microbial inoculum and method for degrading acetaldehyde

A Pseudomonas, nitro technology, applied in the field of microorganisms, can solve the problems of acetaldehyde tolerance and harsh growth environment requirements, and achieve the effects of in-situ pollutant degradation, less interference, and easy operation.

Active Publication Date: 2021-10-15
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of nitroreducing Pseudomonas (Pseudomonas nitroreducens) and a bacterial agent in order to overcome the problem that the acetaldehyde-degrading bacteria in the prior art have strict requirements on acetaldehyde tolerance and growth environment, And their application in degrading acetaldehyde and processing lampblack condensate and the method for degrading acetaldehyde, this bacterial strain can tolerate high concentration acetaldehyde and can under unfavorable conditions (such as containing acetaldehyde and acrolein or acetaldehyde and formaldehyde simultaneously situation) to degrade acetaldehyde, thereby providing an available bacterial source for the biodegradation of acetaldehyde

Method used

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  • Pseudomonas nitroreducens, microbial inoculum, application of Pseudomonas nitroreducens and microbial inoculum and method for degrading acetaldehyde

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Embodiment approach

[0054] According to a specific embodiment of the present invention, the inorganic salt comprises sodium chloride, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium chloride, magnesium sulfate, calcium chloride and ferrous sulfate.

[0055] Wherein, the present invention has no special limitation on the amount of the added inorganic salt, which can be determined according to the type and content of the inorganic salt in the acetaldehyde-containing pollutant. For example, take NH 4 Cl 0.5-4g / L, NaCl4-8g / L, KH 2 PO 4 0.2-0.8g / L, K 2 HPO 4 ·3H 2 O 0.2-0.8g / L, CaCl 2 0.01-0.05g / L, MgSO 4 Taking 0.1-0.5g / L mixed inorganic salt solution as an example, based on each kilogram of the pollutants containing acetaldehyde and / or pollutants contained, the amount of the mixed inorganic salt solution can be 1-3mL / day.

[0056] In the present invention, when the acetaldehyde-containing pollutant is soil, in order to further promote the degradation efficiency of a...

preparation example

[0082] This preparation example is used to prepare the activated seed solution used in the following examples.

[0083] The LB medium was sterilized at 121°C for 20 min and cooled to room temperature. The nitroreductor Pseudomonas strain of the present invention was taken from a -80°C refrigerator, inoculated into the above-mentioned sterilized LB medium, and cultured at 170 rpm and 30°C for 12 hours to obtain an activated seed solution.

Embodiment 1

[0085] This example is used to illustrate the influence of culture conditions on the ability of the strains described in the present invention to degrade acetaldehyde.

[0086] The activated seed solution after activation was inoculated into carbon-free medium with an initial concentration of acetaldehyde of 500 mg / L according to different inoculum amounts (2%, 5%, 8%, 10%, 15%) respectively. Three parallels were set up for each gradient, and the blank culture medium was used as the control. The shake flask was cultured in a constant temperature shaker with a rotation speed of 170r / min and a temperature of 20°C (considering the volatility of acetaldehyde, the temperature was controlled at 20°C), and samples were taken at 0h, 6h, 12h and 24h to determine the amount of acetaldehyde in the medium. Aldehyde degradation rate and OD 600 .

[0087] Take the bacterial solution cultivated to the logarithmic phase and add it to the sterilized and cooled medium with an initial formalde...

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Abstract

The invention relates to the field of microorganisms, and discloses Pseudomonas nitroreducens, a microbial inoculum, application of the Pseudomonas nitroreducens and the microbial inoculum and a method for degrading acetaldehyde, and the preservation number of the Pseudomonas nitroreducens is CGMCC No.23134. According to the present invention, the Pseudomonas nitroreducens can survive and grow in acetaldehyde-containing pollutants, can maintain the acetaldehyde degradation ability in the presence of acrolein, and can coexist with formaldehyde degradation bacteria to achieve the good acetaldehyde degradation effect; and the invention provides an available strain for degrading acetaldehyde in acetaldehyde pollutants by a biological method, can realize in-situ pollutant degradation so as to reduce the cost of acetaldehyde degradation, has the advantages of simplicity and convenience in operation, less interference, less secondary pollution and the like, and is suitable for large-scale fermentation production and long-term use.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Pseudomonas nitroreducens and a bacterial agent, their application in degrading acetaldehyde and treating oil fume condensate, and a method for degrading acetaldehyde. Background technique [0002] Acetaldehyde is a volatile organic compound with a pungent smell and is listed as the first class of carcinogen by the International Agency for Research on Cancer (IARC). Acetaldehyde, one of the most common air toxins, carries a cancer risk greater than one in a million. Acetaldehyde exists widely in nature and is industrially produced on a large scale. And it occurs naturally in coffee, bread and ripe fruit. The routes of human exposure to acetaldehyde include air, water, land or groundwater, as well as beverages and smog. With the improvement of environmental quality and systematic research on the emission characteristics of pollution sources, it has been found that a la...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/38C12R1/07
CPCC12N1/20B01D53/84B01D53/72B01D2258/0283Y02A50/20
Inventor 史纯珍王蓉蓉姚志良
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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