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Method for obtaining chlorogenic acid and isochlorogenic acid from stevia rebaudiana waste residues

A technology for isochlorogenic acid and stevia, which is applied in the field of extracting chlorogenic acid and isochlorogenic acid, can solve the problems of large changes, waste of resources and high production process risks, and achieves the effects of simple method and simple operation.

Inactive Publication Date: 2021-10-15
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional stevia extract production process, only the steviol glycosides in stevia are often obtained, and the chlorogenic acids in stevia are regarded as impurities, and they are removed by physical or chemical methods into precipitated waste residues. If the chlorogenic acid substances in these waste residues are not recovered, it is undoubtedly a waste of resources
[0005] At present, although there are some technical materials, the extraction and separation methods of chlorogenic acid and steviol glycosides in stevia have been proposed, but most of these methods have greatly changed the traditional stevia process. High process risk and long payback period
On the basis of traditional techniques, it is of more practical significance to extract and recover chlorogenic acids in stevia flocculation waste residue, but the prior art methods have less utilization of stevia flocculation waste residue, although the invention 201210421968.2 involves obtaining The method of chlorogenic acid in stevia, but due to the limitation of process conditions, a large amount of isochlorogenic acid is not recycled in stevia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 100kg of stevia rebaudiana dry leaf material and pulverize it, and use hot water as a solvent for countercurrent extraction to obtain a stevia rebaudiana extract. Calcium oxide was slowly added to the stevia extract for flocculation, and the pH value was controlled to be 10 during the flocculation process; after the pH value was stabilized, the flocculation clear liquid and flocculation waste residue were separated by filtering with a plate and frame filter. Stir and disperse the obtained 50 kg stevia flocculation waste residue evenly in 100 kg water, adjust the pH value to 3.5 with hydrochloric acid, and separate the filtrate and filter residue with a plate and frame filter. The filtrate rich in chlorogenic acid is filtered and clarified through a ceramic membrane in turn, and then concentrated and removed by organic membrane filtration, wherein the molecular weight cut-off of the organic membrane is 250, and the membrane passing pressure is 0.4MPa; the membrane co...

Embodiment 2

[0029] Take 100kg of stevia rebaudiana dry leaf material and pulverize it, and use hot water as a solvent for countercurrent extraction to obtain a stevia rebaudiana extract. Calcium hydroxide was slowly added to the stevia extract for flocculation, and the pH value was controlled at 9 during the flocculation process; after the pH value was stabilized, the flocculation clear liquid and flocculation waste residue were separated by filtering with a plate-and-frame filter. Stir and disperse the obtained 50 kg stevia flocculation waste residue evenly in 150 kg water, adjust the pH value to 3.0 with sulfuric acid, and separate the filtrate and filter residue with a plate and frame filter. Filter and clarify the filtrate rich in chlorogenic acid through ceramic membrane in turn, and then concentrate and remove impurities through organic membrane filtration, wherein the organic membrane molecular weight cut-off is 300, and the membrane passing pressure is 0.5MPa; the membrane concentr...

Embodiment 3

[0031] Take 1 t of stevia rebaudiana dry leaf raw materials and pulverize them, and use hot water as a solvent for countercurrent extraction to obtain a stevia rebaudiana extract. Slowly add 50kg of calcium chloride to the stevia extract for flocculation. During the flocculation process, use sodium hydroxide to control the pH value at 11; Stir and disperse the obtained 0.5 t stevia flocculation waste residue evenly in 2 t water, adjust the pH value to 4.0 with citric acid, and separate the filtrate and filter residue with a plate and frame filter. The filtrate rich in chlorogenic acid was filtered and clarified through a ceramic membrane in turn, and then concentrated and removed by organic membrane filtration, wherein the molecular weight cut-off of the organic membrane was 200, and the membrane passing pressure was 0.7MPa; the membrane concentrate was dried to obtain 12.7kg of chlorogenic acid . Disperse and dissolve the filter residue rich in isochlorogenic acid with 1t of...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a method for extracting chlorogenic acid and isochlorogenic acid from stevia rebaudiana waste residues. The invention relates to a method for obtaining chlorogenic acid and isochlorogenic acid from stevia rebaudiana waste residues, which mainly comprises the following steps: by taking industrial production stevia rebaudiana flocculation waste residues as raw materials, dispersing the stevia rebaudiana waste residues with water, regulating the pH value to 3.0-4.0, and filtering and separating filtrate and filter residues; filtering the filtrate with a ceramic membrane and an organic membrane in sequence to remove impurities, and drying to obtain chlorogenic acid; and continuously dissolving and dispersing the filter residue with ethanol, filtering and collecting the filtrate, concentrating under reduced pressure and recovering the ethanol, extracting and refining the extract with ethyl acetate, and drying to obtain the isochlorogenic acid. According to the method, industrial waste residues in the stevia rebaudiana production process are fully utilized, production of stevioside is not affected, meanwhile, chlorogenic acid and isochlorogenic acid are extracted and separated through the waste residues, resin does not need to be used for separation and purification in the whole production process, operation is easy, and the method is suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, in particular to a method for extracting chlorogenic acid and isochlorogenic acid from stevia waste residue. Background technique [0002] Stevia is native to the subtropical regions of South America, and its leaves contain a variety of low-calorie, high-sweet steviol glycosides; it was introduced and cultivated in my country in the 1970s, and stevia extract has been widely used as a sweetener in food at home and abroad , Beverage and other industries. In recent years, research in the field of medicine has also found that stevia extract has various effects such as anti-oxidation, anti-inflammation, lowering blood sugar and blood pressure, and these effects may be more related to chlorogenic acids contained in stevia. [0003] Chlorogenic acids are depsipolic acids produced by caffeic acid and quinic acid, and are a kind of phenylpropanoid compounds produced by shikimic acid pathway in th...

Claims

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Application Information

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IPC IPC(8): C07C67/56C07C67/60C07C67/28C07C67/48C07C67/58C07C69/732
CPCC07C67/56C07C67/60C07C67/28C07C67/48C07C67/58C07C2601/14C07C69/732
Inventor 唐婷覃蒙珍陈久福李杰宋云飞
Owner GUILIN NATURAL INGREDIENTS CORP
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