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A kind of antibacterial and sensitizing active refined product of jujube fruit and its preparation method and application

A sour jujube, active technology, applied in antibacterial drugs, antifungal agents, organic active ingredients and other directions, can solve the problem of poor efficacy of antibiotics against drug-resistant bacteria, and achieve a mild condition and moderate viscosity that are easy to apply to the skin. Effect

Active Publication Date: 2022-06-24
YANAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The first purpose of the present invention is to provide a kind of jujube fruit antibacterial and sensitizing active refined product, which solves the problem of poor curative effect of existing antibiotics on drug-resistant bacteria

Method used

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  • A kind of antibacterial and sensitizing active refined product of jujube fruit and its preparation method and application
  • A kind of antibacterial and sensitizing active refined product of jujube fruit and its preparation method and application
  • A kind of antibacterial and sensitizing active refined product of jujube fruit and its preparation method and application

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Experimental program
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Effect test

preparation example Construction

[0059] A preparation method of an antibacterial and sensitizing active refined product of jujube fruit, comprising the following steps:

[0060] Step 1, making sour jujube fruit into sour jujube powder, using chloroform to extract the sour jujube powder to obtain a chloroform extract;

[0061] Specifically, in step 1.1, take the ripe wild jujube fruit, select the fruit with no mechanical damage, uniform size and color, and no pests and diseases, wash off the surface dirt, put it into an oven for drying at 45°C, pulverize it, and pass it through an 80-mesh sieve to obtain Sour jujube powder, a total of 10kg, put it in a ziplock bag and keep it in the refrigerator for later use.

[0062] Step 1.2, first degreasing the sour jujube powder with petroleum ether, then mix it with chloroform according to the ratio of material to liquid volume ratio of 1:3, and then place it in an ultrasonic cleaner, ultrasonic power 800W, ultrasonic temperature 15-20 ℃, ultrasonic 50min, Filtration, ...

Embodiment 1

[0097] The eluates Fr.1A, Fr.1B, Fr.1C, Fr.1D, Fr.1E, Fr.1F, Fr.1G, Fr.1H, Fr.1I, Fr.1J were respectively mixed with ampicillin (Amp) Combined use to do a combined antibacterial test for Pseudomonas aeruginosa, and to test the activity of the above eluate;

[0098] (1) Preparation of bacterial liquid: shake and cultivate the tested strain at 37°C and 220 rpm overnight, and when the bacterial liquid OD600=1.0, dilute it to 1×10 6 cfu / ml;

[0099] (2) Preparation of each separated component: After accurately weighing each eluate, use absolute ethanol to dissolve and prepare the concentration of 2560mg / ml, 1280mg / ml, 640mg / ml, 400mg / ml, 320mg / ml, respectively. ml, 200mg / ml, 160mg / ml, 100mg / ml, 80mg / ml, 50mg / ml, 40mg / ml, 20mg / ml, 1mg / ml, 0.5mg / ml and 0.25mg / ml stock solutions;

[0100] (3) Preparation of antibiotics: Amp dissolved in sterile water is diluted to a mother liquor with a concentration of 4096 μg / ml using a culture medium, which is filtered and sterilized for subsequ...

Embodiment 2

[0169] The preparation of embodiment 2 erythromycin+Fr.B ointment and its in vivo activity detection

[0170] experiment method

[0171] (1) Preparation of erythromycin + Fr.B antibacterial ointment

[0172] Matrix preparation: weigh 21g of white petrolatum and 8g of liquid paraffin, heat in a water bath to about 60°C to completely melt the matrix and stir evenly;

[0173] Preparation of antibiotic solution: Weigh 1g of erythromycin and dissolve it in 1ml of absolute ethanol, prepare 2 copies for future use;

[0174] Preparation of Fr.B solution: Weigh 0.5g of Fr.B and dissolve it in 1ml of absolute ethanol, prepare 2 copies for later use;

[0175] Ointment preparation: remove the water-bath heating device with the prepared matrix, and when the temperature drops to about 50 °C, add the prepared erythromycin solution, Fr. The proportion of Fr.B is 1%, the proportion of Fr.B is 0.5%, and the mixture is stirred clockwise until uniform, and meanwhile the temperature of the mixe...

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Abstract

The invention discloses an antibacterial and sensitizing active refined product of wild jujube fruit and its preparation method and application. Fr.A was obtained by column chromatography; the treated Fr.A was separated by silica gel column chromatography to obtain Fr.B, and Fr.B was concentrated by distillation and vacuum-dried to obtain Fr.B, an antibacterial and sensitizing activity refined product of jujube fruit. Fr.B enhances the bactericidal effect of antibiotics on drug-resistant bacteria by changing the permeability of cells. It has broad-spectrum antibacterial and sensitizing activities, and can play an anti-inflammatory and wound-healing role.

Description

technical field [0001] The invention belongs to the technical field of jujube extracts, relates to a refined product with antibacterial and sensitizing activity of jujube fruit, and also relates to a preparation method and application of the above refined product. Background technique [0002] In recent years, in the treatment of bacterial infections, the inappropriate use of antibiotics has led to the emergence of widely drug-resistant bacteria, resulting in poor efficacy of existing antibiotics against drug-resistant bacteria. In view of the continuous emergence of bacterial drug resistance, it is urgent to find new and effective methods to solve the problem of drug resistance. Among them, plant natural compounds are important potential sources for the development of new antibacterial drugs, and many plants have been studied and can function as antibacterial sensitizers. The characteristics of this class of drugs are that they have no or only weak antibacterial effects, b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/201A61K31/045A61K31/20A61K31/01A61K31/231A61K31/11A61K31/23A61K31/19A61K31/198A61K31/22A61P31/04A61P29/00A61P17/02A61K36/725A61K9/06A61P31/10A61K45/06A61K31/7036A61K31/702A61K31/165A61K31/7048A61K31/56A61K31/496
CPCA61K31/201A61K31/045A61K31/20A61K31/01A61K31/231A61K31/11A61K31/23A61K31/19A61K31/198A61K31/22A61P31/04A61P29/00A61P17/02A61K36/725A61K9/06A61K9/0014A61P31/10A61K45/06A61K31/7036A61K31/702A61K31/165A61K31/7048A61K31/56A61K31/496A61K2236/35A61K2236/55A61K2236/51A61K2300/00
Inventor 林金水张恒尹芮高倩倩杨建社成娟丽张向前王凤裴永福惠雪芳
Owner YANAN UNIV
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