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Pseudomonas parafulva and application thereof

A technology of pseudomonas and microbial strains, which is applied to Pseudomonas paraxanthinum and its application fields in plant growth promotion and soil heavy metal pollution prevention and control, can solve problems such as few reports of pseudomonas, and achieves Improve repair efficiency, promote growth, and promote plant growth effects

Active Publication Date: 2021-10-08
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Pseudomonas fluorescens (P.fluorescens), Pseudomonas aeruginosa (P.aeruginoda), Pseudomonas putida (P.putida) and Pseudomonas syringae (P.syringae) are the most studied , while there are few reports on other species in Pseudomonas

Method used

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  • Pseudomonas parafulva and application thereof
  • Pseudomonas parafulva and application thereof
  • Pseudomonas parafulva and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and Identification of Pseudomonas Paraxanthomonas CNBG-PGPR-7

[0026] (1) Culture medium composition:

[0027] Nutrient Agar (NA) medium: peptone 10g, beef extract powder 3g, sodium chloride 5g, agar 15g, distilled water 1L.

[0028] (2) Sample dilution and coating

[0029] Take 0.5g soil sample (collected from the farmland around the Nanjing mining area in Jiangsu Province) and suspend and shake in 4.5mL sterile saline, which is 10 -1 Gradient (ten-fold dilution), take 0.5mL from the dilution and suspend and shake in 4.5mL sterile normal saline, which is 10 -2 Gradient (100-fold dilution), and so on until diluted to 10 -6 gradient. Draw 100 μL from the dilutions of the 6 dilution gradients, spread them on the Nutrient Agar (NA) solid medium plate, prepare 2 copies in parallel, invert them, and culture them in a constant temperature incubator at 30°C for 24-36 hours, observe from time to time .

[0030] (3) Strain isolation and purification

...

Embodiment 2

[0052] Physiological characteristics of embodiment 2 bacterial strains

[0053] The medium used in this example is Nutrient Broth (NB) medium. The composition of NB medium is: peptone 10g, beef extract powder 3g, sodium chloride 5g, distilled water 1L.

[0054] (1) Growth curve

[0055] Take out the single colony of the strain CNBG-PGPR-7 picked from the solid medium plate and transfer it into the above NB liquid medium for activation, then activate the 2nd generation with 5% inoculum amount, and finally insert the 4th generation NB liquid with 5% inoculum amount In the culture medium, place it on an automatic growth curve analyzer at 30°C for continuous culture for 72 hours, and record the OD600 value every 0.5 hours. The growth curve of Pseudomonas paraxanthomonas CNBG-PGPR-7 is shown in figure 2 , the strain can enter the mid-logarithmic growth phase at about 5.0h, the OD600 value is 1.00, and then the growth rate slows down, and reaches the early stage of stabilization...

Embodiment 3

[0060] Example 3 Evaluation of related indicators of bacterial strain growth-promoting ability

[0061] (1) Nitrogen fixation capacity

[0062] Ashby nitrogen-free solid medium: glucose 10g, potassium dihydrogen phosphate 0.2g, magnesium sulfate heptahydrate 0.2g, sodium chloride 0.2g, calcium sulfate dihydrate 0.1g, calcium carbonate 5g, agar 20g, distilled water 1000mL.

[0063] Pick a single colony from the NA solid medium plate and inoculate it on Ashby nitrogen-free solid medium. Plate streaking method: Divide the Ashby nitrogen-free medium into three areas I, II, and III; pick a single colony from the NA solid medium, and perform zigzag streaking in the I area of ​​the Ashby nitrogen-free medium; Dip the bacterial solution from the zone, and make a zigzag streak in the II zone of the Ashby nitrogen-free medium; dip in the bacterial fluid from the II zone, and perform a zigzag streak in the III zone of the Ashby nitrogen-free medium. The inoculated Ashby nitrogen-free s...

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Abstract

The invention discloses a Pseudomonas parafulva and application thereof. The Pseudomonas parafulva was preserved in China General Microbiological Culture Collection Center on November 6, 2020, and the preservation number is CGMCC No.21121. The Pseudomonas parafulva CNBG-PGPR-7 has good tolerance to various heavy metals at the same time, and has a plant growth promoting function and nitrogen fixation, phosphorus solubilization and siderophore secretion capabilities; the germination rate of sage seeds can be promoted to be increased by about 10%, the biomass of the upper portion of sage grassland is increased by 20% to 30%, and the biomass of underground root systems is increased by 11% to 65%; and the tolerance of sage to heavy metals can be improved, and the remediation efficiency is improved. The Pseudomonas parafulva can be used for removing heavy metals in heavy metal polluted environments and can be inoculated to heavy metal tolerant or hyperaccumulator rhizosphere to improve the remediation efficiency of heavy metal polluted soil.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Pseudomonas paraxanthomonas and its application in plant growth promotion and soil heavy metal pollution prevention and control. Background technique [0002] Bacteria are an important class of microbial resources in the environment, with the characteristics of various types, wide distribution, large specific surface area, fast reproduction and vigorous metabolism. Certain bacteria can promote plant growth, increase plant stress resistance, and even inhibit harmful microorganisms. Such bacteria are called plant growth-promoting bacteria. Plant growth promoting bacteria promote plant growth through direct or indirect effects. The direct growth-promoting effect of plant growth-promoting bacteria is mainly through the synthesis of certain substances that have a direct effect on plant growth and development, such as plant growth hormone (IAA), iron cells and othe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P21/00C09K17/14B09C1/10C02F3/34C12R1/38C02F101/20
CPCA01N63/27C09K17/14B09C1/10C02F3/34C02F2101/20Y02W10/10
Inventor 贾明云于金平吕世鹏周冬琴李琦侯炤琪刘壮壮丰柳春
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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