Preparation method and application of acid-resistant lactobacillus caseiwith high reproductive capacity

A technology of Lactobacillus casei and ability, applied in the field of microorganisms, to achieve enhanced resistance, increased maximum specific growth rate, and improved vitality

Pending Publication Date: 2021-09-24
陈玉松
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of nucleotides to prepare probiotics with acid tolerance and strong reproductive ability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of acid-resistant lactobacillus caseiwith high reproductive capacity
  • Preparation method and application of acid-resistant lactobacillus caseiwith high reproductive capacity
  • Preparation method and application of acid-resistant lactobacillus caseiwith high reproductive capacity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The influence of embodiment 1 exogenous nucleotide 5' disodium guanylate on the growth of Lactobacillus casei

[0026] After the strains preserved in the glycerol protectant were activated in the MRS liquid medium and continuously cultured for 3 generations, the MRS medium was used as a blank control, and 1g / 100mL, 2g / 100mL, 3g / 100mL, 4g / 100mL of 5' Disodium guanylate was inoculated with Lactobacillus casei at a volume ratio of 1%, cultured on a shaker at 37°C for 24 hours, and samples were taken every 4 hours. The live bacteria of Lactobacillus casei were counted by the double dilution method, and the growth curve was drawn. The modified Gompetz model and the modified Logistic model were used to describe the growth and variation of Lactobacillus casei nonlinearly.

[0027] (1) Modified Gompertz model

[0028]

[0029] In the formula, t is time (h), N(t) is the number of bacteria at time t, N max , N 0 is the maximum and initial bacterial count (CFU / g). mu max i...

Embodiment 2

[0035] The influence of embodiment 2 exogenous nucleotides on Lactobacillus casei biofilm, extracellular polymer

[0036] Lactobacillus casei (1% inoculum) was inoculated in MRS broth culture medium for 24 hours on a shaker (37°C, 160r / min), diluted at a ratio of 1:100, and 100 μL of bacterial solution and 4.0% 5'guanosine Disodium acid MRS medium was added to the well plate, and MRS broth medium was used as a control. Put sterile coverslips and polished zinc sheets (0.5mm×0.5mm×0.3mm) into the orifice plate, and after standing at 37°C for 48 hours, wash the coverslips slowly with sterile water for 3 times, crystal violet ( 0.4%) was stained for 20 minutes, and the changes of the bacterial biofilm were observed under a microscope. Rinse the zinc sheet slowly with sterile water to remove non-adherent bacteria. Fix with 2.5% glutaraldehyde for 30 min and then dry for 30 min. Raman spectroscopy was used to detect the effect of exogenous nucleotides on the extracellular polymer...

Embodiment 3

[0039] Example 3 Molecular docking study on the mechanism of action of disodium 5' guanylate

[0040] The biofilm and extracellular polymers of Lactobacillus casei can be increased by inducer-2 (AI-2). AI-2 is a signal molecule synthesized through the methyl cycle. Lux S protein is the key enzyme for the synthesis of AI-2 , so it is of great significance to study the interaction between disodium 5'guanylate and lux S. Molecular docking is a theoretical simulation method that mainly studies the interaction between molecules (such as ligands and receptors) and predicts their binding mode and affinity. In recent years, molecular docking has become an important technology in the field of computer-aided drug research. In this example, the interaction between 5' disodium guanylate, AI-2 type signal molecule and the active site of Lactobacillus casei LuxS receptor protein was studied. The result is as Figure 4 As shown, Figure A is the interaction between AI-2 signal molecules and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method and application of acid-resistant lactobacillus casei with high reproductive capacity, and belongs to the technical field of microorganisms. According to the invention, lactobacillus casei is inoculated into an MRS liquid culture medium added with disodium 5 '-guanylate, and is cultured at 30-37 DEG C for 10-48 hours, so that the acid-resistant lactobacillus casei with high reproductive capacity is obtained. According to the lactobacillus casei, the production of a biofilm and the secretion of extracellular polymeric substances are increased, and the resistance of the lactobacillus casei to an external adverse environment is enhanced, so that the survival rate of bacteria is enhanced, a new idea is provided for improving the survival rate of the lactobacillus casei, and the application of the lactobacillus casei in food and health food is expanded.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method and application of acid-resistant Lactobacillus casei with strong reproductive ability. Background technique [0002] Probiotics are active microorganisms that colonize the human body and play an important role in human health. Studies have found that probiotics play an important role in regulating the balance of intestinal flora, promoting nutrient absorption, and maintaining intestinal health. However, probiotics are easily inactivated during processing and sales and when they pass through the digestive tract, and their functional dosage cannot be reached. Therefore, it is necessary to find suitable prebiotic substances to promote their growth and reproduction or enhance their ability to resist external adverse environments. [0003] Nucleotide is an important component of cells, the precursor of DNA and RNA, and plays an important role ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A23L33/135C12R1/245
CPCC12N1/20A23L33/135A23V2002/00A23V2400/125A23V2200/3204A23V2200/3262A23V2200/326A23V2200/324
Inventor 李勇丁婷陈玉松
Owner 陈玉松
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap