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Fermentation culture method for producing schizophyllan from schizophyllum commune

A technology for fermenting and culturing Schizophyllum, which is applied in the field of microbial fermentation, can solve problems such as inability to meet the needs of polysaccharide production, and achieve the effects of increasing yield, increasing yield and increasing content

Active Publication Date: 2021-09-21
WUZHOUFENG AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both jasmonic acid and methyl jasmonate increase polysaccharide production by increasing the activity of glycosyltransferases (Jaulneau, Valérie, et al."Ulvan, a sulfated polysaccharide from green algae, activates plant immunity through the jasmonic acid signaling pathway. "Journal of Biomedicine and Biotechnology 2010(2010).), but still can not meet the demand of polysaccharide production

Method used

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  • Fermentation culture method for producing schizophyllan from schizophyllum commune
  • Fermentation culture method for producing schizophyllan from schizophyllum commune
  • Fermentation culture method for producing schizophyllan from schizophyllum commune

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Preparation of soybean meal protein.

[0028] The following four schemes A, B, C, and D are used to prepare soybean meal protein respectively, and the parts mentioned refer to parts by mass.

[0029] A: Soak 80 parts of soybean meal with 300 parts of water for 24 hours; adjust the pH value to 6-7, add papain according to 2% of the weight of soybean meal, stir evenly, and carry out enzymatic hydrolysis at 55° C. for 0.5 hour. The enzymatic hydrolyzate was deactivated at 100° C. for 10 minutes, and soybean meal protein A was obtained after the treatment.

[0030] B: Soak 80 parts of soybean meal in 300 parts of water for 24 hours; adjust the pH value to 6-7, add papain according to 2% of the weight of soybean meal, stir evenly, and carry out enzymatic hydrolysis at 55° C. for 1 hour. The enzymolyzate was deactivated at 100° C. for 10 minutes, and soybean meal protein B was obtained after the treatment.

[0031] C: Soak 80 parts of soybean meal in 300 parts...

Embodiment 2

[0034] Example 2: Initial culture of Schizophyllum.

[0035] Schizophyllum commune SCSIO z046 was inoculated with PDA comprehensive medium (potato 200g, glucose 20g, agar 15-20g, water 1000mL, pH natural), and cultured at 30°C in the dark for 5-10 days after inoculation.

Embodiment 3

[0036] Example 3: Optimization of SPG basal medium.

[0037] The production of SPG was optimized using the experimental method of Design Expert version 7.0.1.0 (Statcon, USA). The independent variables selected for optimization were as follows: starch, soybean meal protein prepared by Protocol C of Example 1, yeast extract, sodium carboxymethylcellulose (CMC) and oleic acid. Table 1 presents the factors evaluated at five different levels. The total experiment was 50 runs, including 32(2 5 ) factor points, 10 axial points, and 8 replicates of the center point. The dependent response is the SPG yield (y 1 ) and biomass production (y 2 ).

[0038] Table 1: Experimental independent variables and their levels in the central composite design (CCD)

[0039]

[0040] Effective factors such as carbon and nitrogen sources and appropriate concentration ranges were investigated in this study through a one-factor-at-a-time approach, as considered in previous studies. Based on the...

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Abstract

The invention belongs to the field of production of high-added-value products by microorganisms, and particularly relates to a fermentation culture method for producing schizophyllan from schizophyllum commune. The method comprises the following steps of: performing deep liquid culture on schizophyllum commune by using a basic culture medium, and then adding cyclopentanone fatty acid to continue culture. The fermentation culture medium and the cyclopentanone fatty acid additive provided by the invention can shorten the fermentation period of schizophyllum commune hyphae, and greatly improve the content of high-added-value products produced by unit mycelium.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for promoting high-level secretion of schizophyllum polysaccharide by schizophyllum. Background technique [0002] Polysaccharides are abundant natural biopolymers and represent the largest polymers ever produced globally. Schizophyllan polysaccharide (SPG), as a biopolymer, is a nonionic and water-soluble extracellular polysaccharide consisting of a backbone of 1,3-β-D-glucopyranose units linked to a single 1,6- β-D-glucopyranose linking. This biopolymer is produced by the wood-decaying and filamentous fungus Schizophyllum strains (Kumari, Maushmi, Shrikant A. Survase, and Rekha S. Singhal. "Production of schizophyllan using Schizophyllum commune NRCM." Bioresource technology 99.5 (2008): 1036-1043. Alizadeh, Vahid, Seyed AbbasShojaosadati, and Seyed Morteza Zamir."Enhancement of schizophyllan production in Schizophyllum commune using microparticles i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/14C12P19/04C12R1/645
CPCC12N1/38C12N1/14C12P19/04
Inventor 迟艳李峰刘昌春张宇
Owner WUZHOUFENG AGRI SCI & TECH
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