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Penicillium bilaiae MP6 with bacteriostatic action and application thereof

A technology with Penicillium baileyi and bacteriostasis, applied in the field of microorganisms, can solve the problems of destroying ecological balance, affecting the sustainable and healthy development of agriculture, environmental pollution, etc., and achieves the effect of simple use, good market application prospects and good effects.

Active Publication Date: 2021-09-17
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the prevention and control of diseases caused by plant pathogenic bacteria mainly relies on chemical pesticides. The excessive use of chemical agents not only causes excessive pesticide residues in agricultural products, endangers the health of consumers, but also causes environmental pollution, destroys ecological balance, and affects the sustainable and healthy development of agriculture.

Method used

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  • Penicillium bilaiae MP6 with bacteriostatic action and application thereof
  • Penicillium bilaiae MP6 with bacteriostatic action and application thereof
  • Penicillium bilaiae MP6 with bacteriostatic action and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Isolation, screening and identification of bacterial strains

[0039] 1. Isolation and screening of strains

[0040] In July 2018, 15 vegetable soil samples were collected in Jimo, Qingdao, and the samples were transferred to sterile water and dispersed with glass beads. then diluted with sterile water to 10 -4 ~10 -7 Take 200 μl of PDA-coated plates (take 200 grams of potatoes, wash and cut into pieces, add appropriate amount of water and boil for 20-30 minutes, filter the potato juice with gauze, add 20 grams of glucose, 20 grams of agar, and dilute to 1000 ml. Natural pH), cultured at 26~28°C.

[0041] Pick a single colony of mold, and use the plate confrontation growth method to inoculate the single colony and Fusarium oxysporum, Phytophthora nicotiana, Phytophthora capsici, and Phytophthora soybean on PDA plates respectively, and culture at 26-28°C. By calculating the mycelial growth inhibition rate of test molds on plant pathogens, a strain with ...

Embodiment 2

[0053] Example 2 The biocontrol experiment of Penicillium balai MP6

[0054] 1. Inhibitory effect of MP6 strain on mycelia growth of plant pathogens

[0055] Experimental method: Put the MP6 strain and 4 kinds of pathogenic bacteria (Fusarium oxysporum, Phytophthora tabacum, Phytophthora capsici, Phytophthora soybean) respectively on the PDA plate, 26 ~ 28 ℃ activation culture for 3 ~ 5 days; Take a circular fungus cake (0.60 cm in diameter) from the edge of the mycelium of the activated strain (the growth status should be as consistent as possible), and then use an inoculation needle to pick the fungus cakes of the MP6 strain and the test Phytophthora to the two ends of the new plate. Then place the culture dish upside down in an incubator (26-28°C) for cultivation. After the treatment, observe and measure the growth of mycelia in time, and the antibacterial effect is as follows: Figure 2-5 As shown, the results showed that the MP6 strain had a good inhibitory effect on th...

Embodiment 3

[0067] Embodiment 3: Penicillium balai MP6 inhibits the growth of plant pathogen Ralstonia solanaceare

[0068] Dip the R. solanacearum bacterial solution with a sterile cotton swab, spread it on the surface of the plate, and then inoculate the Penicillium belaiai MP6 strain. Place the petri dish upside down in an incubator (26-28°C) and incubate for 4-5 days to determine the antibacterial effect of MP6 strain on R. solanacearum. Test results such as Figure 11 As shown, it is found that there is an obvious inhibition zone around the bacterium colony of the MP6 strain, indicating that Penicillium belais MP6 also has a significant inhibitory effect on the growth of R. solanacearum.

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Abstract

The invention discloses a Penicillium bilaiae MP6 with bacteriostatic action and application thereof. The Penicillium bilaiae MP6 is classified and named as Penicillium bilaiae, and is preserved in China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 2021612; the bacterial colony of the Penicillium bilaiae MP6 has developed hyphae and short length, and is felty; and the conidiophore head is broom-shaped, the conidium is chain-like, and the nucleotide sequence of ITS is shown as SEQ ID No. 5. The Penicillium bilaiae MP6 can be used for preparing a biocontrol agent for preventing and treating blight, phytophthora and / or bacterial wilt, and has obvious effects of inhibiting fusarium oxysporum, phytophthora sojae, phytophthora nicotianae, phytophthora capsici and ralstonia solanacearum, so that the resistance of plants to blight, phytophthora or bacterial wilt is increased, and the Penicillium bilaiae MP6 has good market application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Penicillium belaisi MP6 with antibacterial effect and its application. Background technique [0002] At present, due to the ubiquity of continuous cropping in agricultural production, the root metabolites of plants gradually accumulate in the soil, which provides an environment for the growth and reproduction of soil-borne fungal diseases, resulting in the increasing occurrence of soil-borne diseases in crops and vegetable cultivation. After crops are infected by soil-borne pathogenic fungi, the symptoms are generally blight-type and wither-type, and the specific symptoms are plant wilting, which in turn causes major economic losses. Among them, Fusarium, Phytophthora and Ralstonia solanacearum are three important soil-borne pathogenic fungi. [0003] Fusarium oxysporum is a worldwide distribution of soil-borne pathogenic fungi with a wide range of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/36A01P1/00A01P3/00C12R1/80
CPCC12N1/14A01N63/36
Inventor 梁文星徐敏王光远杨震宙李德龙李敬涛
Owner QINGDAO AGRI UNIV
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