Sandy slope microorganism curing liquid and sandy slope treatment method
A technology of microorganisms and solidified liquid, applied in the field of ecological management, can solve the problems of high cost, affecting the treatment effect, and poor growth conditions.
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Embodiment 1
[0050] This embodiment provides a method for preparing a microorganism cured liquid.
[0051] Preparation of (1) Bacillus pasteurii bacteria: in a sterile environment, picked with a sterile inoculating loop Bacillus pasteurii a ramp ring strain, transferred to seed medium, 30 deg.] C, 150rpm shaking for 24h under conditions , Bacillus pasteurii obtain seed liquid; press 2% inoculum was inoculated in a liquid medium and cultured to an OD600 value of 1.0 when the end of the incubation, bacteria standby.
[0052] Bacillus pasteurii seed medium consisted of 20g / L tryptone, 20g / L urea, 4g / L of sodium chloride, and deionized water, the pH is adjusted using pH adjusting agent is 9.0.
[0053] Bacillus pasteurii broth by extraction with 20g / L of yeast extract, 15g / L sucrose, 2g / L of ammonium sulfate, and distilled water, adjusting the pH using pH adjusting agent is 9.0 to 9.2.
[0054] (2) Preparation of staphylococcal bacteria: in a sterile environment, with a sterile inocula...
Embodiment 2
[0068] The present embodiment were used in Example 1, the cured liquid microorganism Comparative Examples 1 to 3. SLOPE simulation. Wherein, the slope is a slope of 1: 1, the slope of sandy soil particle diameter of 2 ~ 5mm.
[0069] Treatment: according to 350L / m 3 Standard, the nutrient solution is sprayed on the slope, after 15min accordance 100L / m 3 Standard mixing uniformly sprayed bacteria, in this order, every morning, once in the afternoon each spraying, spraying consecutive 5 days.
[0070] 5 days after spraying, the thickness of the consolidated soil detection embodiment of Example 1 of 6.87cm, thickness of the consolidated soil Comparative Example 1 was 5.16cm, soil consolidation Comparative Example 2 has a thickness of 4.63cm, thickness of the consolidated soil of Example 3 3.54cm. Show an example of mixed bacteria embodiment, the curing effect can effectively improve the soil.
Embodiment 3
[0072] The present embodiment were used in Example 1, the cured liquid microorganism Comparative Examples 1 to 3. SLOPE simulation. Wherein, the slope is a slope of 1: 0.5, particle diameter of sandy soil slope of 10 ~ 15mm.
[0073] Treatment:
[0074] (1) is inserted into the grouting pipe injected at sandy slope: THEⅡ grouting material is sand consolidation agent, water cement ratio is 0.4; grouting shaved cone-shaped distal end of the catheter, the pulp having a drainage hole quincunx seamless steel pipe, diameter 32mm, thickness 3.25mm, 1.8m, the level of elevation of 10 degrees catheter length, circumferential spacing 30cm, the longitudinal spacing of 0.5m, pulp drain aperture 6mm, hole spacing 10cm, grouting pressure 0.4Mpa.
[0075] (2) grouting 1.5h, microbial spray liquid curing method according to the second embodiment.
[0076] (3) 5 days after spraying, top-down manner laid mat reinforced microphone, between the web and the use of the same material as pad Mike reinfor...
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