Preparation method of phosphorene-functional-modified 3D-print polylactic acid bionic nanofiber support

Abstract

The invention discloses a preparation method of a phosphorene-functional-modified 3D-print polylactic acid bionic nanofiber support and aims to provide a multifunctional support material for repairing bone defects and a preparation method thereof. The preparation method is characterized by comprising the following steps: loading an anti-inflammatory drug ibuprofen onto nano flaky phosphorene prepared by a liquid-phase stripping method, and coating the surface of the phosphorene with a polydopamine coating, so as to realize intelligent controlled release of the drug; and uniformly doping the obtained drug-loaded phosphorene into an amination-modified polylactic acid matrix to obtain a size mixture, constructing a personalized support by adopting a low-temperature 3D printing technology, and enabling the support to be subjected to phase separation under a low-temperature printing condition so as to form a reticular nanofiber structure of a bionic extracellular matrix. The preparation method has the characteristics that the prepared support can be subjected to personalized designing according to characteristics of a bone defect part of a patient, and the prepared support has good actions of cellular affinity, photothermal conversion performance, bone growth promotion, intelligent drug release and long-acting inflammation resisting and has potential application prospects in the field of bone tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of bone repair biomaterials, and in particular relates to a preparation method of a 3D printed polylactic acid biomimetic nanofiber scaffold functionalized and modified by phosphorene. Background technique [0002] Surgical intervention is required when bone tissue is extensively damaged and cannot heal itself, and bone tissue engineering has been widely studied as an attractive strategy for the repair and reconstruction of bone defects. Tissue engineering scaffolds as seed cell carriers are the key to tissue engineering technology, so constructing scaffolds with good biological activity and osteogenic ability has become a research focus in the field of bone tissue engineering. In addition to having good activity, the tissue engineering scaffold used for bone tissue repair must also have an interpenetrating three-dimensional pore structure similar to natural bone tissue to facilitate cell implantation, migra...

AI Technical Summary

Problems solved by technology

Polylactic acid material has good biocompatibility and biodegradability, and has been widely used in the preparation of tissue engineering scaffolds, but its application as a bone tissue engineering scaffold material also has some inherent performance deficiencies: first, polylactic acid Lactic acid is a strong hydrophobic material, which is not conducive to the adhesion and proliferation of cells on its surface, so the cell affinity is poor and the biological activity is low; secondly, the molecular structure of polylactic acid lacks active sites for inducing osteogenic mineralization, so bone Poor inductivity, low osteogenic ability, and the high temperature required for the preparation of scaffolds by the FDM method will degrade the polylactic acid material, and the acidic monomers released by the degradation will cause non-infectious inflammatory reactions at the implantation site, resulting in implantation failure of surgery

However, the chemical stability of phosphorene is poor, and it is easy to react with water vapor and oxygen in the air to oxidatively degrade, resulting in poor photothermal performance.