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Preparation method of fluorescent coding microsphere coated with metal nano shell layer

A metal nanometer, fluorescent encoding technology, applied in the field of preparation, fluorescent encoding microspheres, can solve the problems of low surface coverage, uncontrollable LSPR peaks, complex preparation process and so on

Active Publication Date: 2021-09-03
HANGZHOU SHINEDO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the common problems faced by these preparation methods are: low surface coverage, poor uniformity, complicated preparation process and uncontrollable LSPR peaks, etc.

Method used

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  • Preparation method of fluorescent coding microsphere coated with metal nano shell layer
  • Preparation method of fluorescent coding microsphere coated with metal nano shell layer
  • Preparation method of fluorescent coding microsphere coated with metal nano shell layer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] figure 1 It is a preferred embodiment of the method for preparing fluorescently encoded microspheres coated with noble metal nanoshells provided by the present invention. The fluorescently encoded microspheres coated with noble metal nanoshells prepared in this example are CdSe / ZnS QDs / PSMA@Ag nanoshell The noble metal nanomaterial is Ag nanoparticles, the fluorescent coding material is CdSe / ZnS quantum dots, and the polymer is styrene-maleic anhydride copolymer (PSMA). They are divided into two groups, and the preparation method is SPG film. Emulsion method and Pickering emulsion method, the specific preparation steps are:

[0088] Divided into two groups, the first group:

[0089] Dissolve 0.05mg of CdSe / ZnS quantum dots (purchased from Suzhou Xingshuo Nanotechnology Co., Ltd.) and 0.5g of styrene-maleic anhydride copolymer (PSMA) in 8mL of toluene to form a dispersed phase;

[0090] Disperse 60 mg of Ag resuspended in ethanol (90% ethanol) with nanoparticles (100 ...

Embodiment 2

[0101] The fluorescence-encoded microspheres (group 1) coated with noble metal nanoshells prepared in this example are CuInS 2 / ZnSQDs / PSMA@Ag nanoshell, where the noble metal nanomaterial is Ag nanoparticles and the fluorescent encoding material is CuInS 2 / ZnS quantum dots, the polymer is styrene-maleic anhydride copolymer (PSMA), and a group of metal-free nano-shells are performed at the same time, and sodium dodecyl sulfate (SDS) is used as a surfactant to prepare fluorescently encoded microstructures. Ball, as control group (second group), preparation method is SPG-Pickering emulsion method, and concrete preparation steps are:

[0102] Divided into two groups, the first group:

[0103] 1 mg of CuInS 2 / ZnS quantum dots and 0.5g of styrene-maleic anhydride copolymer (PSMA) are dissolved in 8mL of toluene to form a dispersed phase;

[0104] Disperse 75mg of Ag nanoparticles (80nm particle size) resuspended in ethanol in 100mL of ultrapure water, ultrasonically disperse e...

Embodiment 3

[0117] The fluorescence-coded microspheres coated with noble metal nanoshells prepared in this example are HPS / MOTAS@Aunanoshell, wherein the noble metal nanomaterials are Ag nanoparticles, and the fluorescent coded materials are 1,1,2,3,4,5-6 Phenylsilole (HPS), the polymer is styrene-acrylic acid copolymer (MOTAS), and the preparation method is the SPG-Pickering emulsion method, and the specific preparation steps are:

[0118] 0.8 mg of HPS and 0.5 g of styrene-acrylic acid copolymer (MOTAS) were dissolved in 8 mL of toluene to form a dispersed phase;

[0119] Disperse 100mg of Ag nanoparticles resuspended in ethanol in 100mL of ultrapure water, and disperse uniformly by ultrasonic to form a continuous phase;

[0120] A SPG membrane with a specification of Φ10xL20mm (effective length 10mm) is used, and the membrane pore size is 3 μm. An SPG membrane emulsification device (purchased from Beijing Jiasheng Xingye Technology Co., Ltd., imported from Japan, model: MN-20) is used,...

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Abstract

The invention provides a preparation method of a fluorescent coding microsphere coated with a metal nano shell layer. Different types of fluorescent materials with different contents are doped into a polymer microsphere by using a metal nano material modified with a certain ligand as a new surfactant in an emulsification process through an SPG method so as to prepare the fluorescent coding microsphere with different fluorescent coding signals inside and uniformly coated with the metal nano shell layer on the surface in one step. The prepared fluorescence coding microsphere comprises the metal nano shell layer, a polymer and a fluorescence coding material, the particle size of the fluorescence coding microsphere is 1-20 [mu]m, the coefficient of variation (CV) is less than 10%, and the fluorescence coding microsphere can be used for detecting protein / nucleic acid. The preparation method is simple in preparation process, high in surface coating rate, good in uniformity and controllable in LSPR peak, and the problems of low surface coating rate, poor uniformity, complex preparation process, uncontrollable local surface plasma resonance (LSPR) peak and the like in the current common precious metal nano shell coating method are solved.

Description

technical field [0001] The invention relates to the field of preparation and application of micro-nano materials, in particular to fluorescence-encoded microspheres coated with metal nano-shells, a preparation method and applications thereof. Background technique [0002] Liquid-phase biochip technology uses encoded microspheres as the carrier of liquid-phase reactions, and according to the interaction rules of protein-protein, nucleic acid-nucleic acid, etc., uses fast and high-throughput flow cytometry as an analysis method to analyze proteins, nucleic acids, etc. Rapid and high-throughput multiplex quantitative detection has a very wide range of applications in the fields of protein expression, nucleic acid analysis, early diagnosis and prognosis of diseases. Compared with traditional detection methods, although liquid-phase chip technology has achieved higher detection sensitivity, with the rapid development of nanoscience and nanotechnology, especially in the face of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06C09K11/62C09K11/88C12Q1/689C12Q1/6837C12Q1/04G01N33/58G01N33/574C12R1/35
CPCC09K11/883C09K11/623C09K11/06C09K11/025C12Q1/689C12Q1/6837G01N33/582G01N33/585G01N33/57473C09K2211/1096C12Q2563/107C12Q2563/149C09K11/565C09K11/58C09K11/621C09K2211/1007C09K11/02Y02A50/30B82Y15/00B82Y20/00B82Y30/00B82Y40/00G01N21/25G01N2021/258B01F23/43B01F23/49B01F23/4105B01F33/45B01F23/06B01F23/48B01F23/4146B01J13/04B01J13/02
Inventor 方剑秋钟春梅
Owner HANGZHOU SHINEDO BIOTECH CO LTD
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