Method for reducing total mercury content in rice

A rice and strain technology, applied in microorganism-based methods, horticultural methods, rice cultivation, etc., can solve the problems of complex preparation of sodium selenite-modified biochar, damage to soil ecosystems, and high energy consumption for biochar preparation. , to achieve the effect of less workload, simple spraying operation and less environmental damage

Active Publication Date: 2021-08-27
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The inventive method can effectively reduce the absorption of inorganic mercury and methylmercury by rice, but the disadvantage of this method is that the preparation of sodium selenite modified biochar is relatively complicated, the cost is high, and it is not environmentally friendly, and sodium selenite is highly toxic , which may harm the soil ecosystem
This method can reduce the absorption of mercury by crops, but the disadvantage of this method is that the effect of non-fine treatment in the field is not clear, and this soil conditioner needs to be added every time rice is planted, and long-term addition of mineral materials will change the soil structure. risks of
Chinese patent CN 108178709 A discloses a preparation method and application method of a rice mercury absorption inhibitor, wherein the rice mercury absorption inhibitor includes prepared biochar, and the application method is mainly to combine rice mercury absorption inhibitor with nitrogen fertilizer, The basal fertilizer composed of phosphate and potassium fertilizers is buried in the plow layer of mercury-contaminated soil and then rice is planted. This method can reduce the enrichment of inorganic mercury in rice and inhibit the absorption of methylmercury by rice. However, the disadvantage of this method is that biochar The preparation of high energy consumption

Method used

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  • Method for reducing total mercury content in rice
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  • Method for reducing total mercury content in rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The detection of the bacteria Pseudomonas taiwanensis used in the present invention

[0031] (1) Form

[0032] Bacteria Pseudomonas taiwanensis microscope (1000X) observation photo used in the present invention sees figure 1 , see plate colony morphology figure 2 .

[0033] (2) 16S rRNA gene sequence analysis

[0034] ①16S rRNA gene sequence

[0035]

[0036]

[0037] ②16S rRNA gene sequencing BLAST results

[0038] 16S rRNA gene sequencing BLAST results are shown in Table 1.

[0039] Table 1 Bacteria Pseudomonas taiwanensis 16S rRNA gene sequencing BLAST result used in the present invention

[0040]

[0041]

[0042]

[0043]

[0044]

[0045] ③Evolutionary tree

[0046] evolutionary tree image 3 .

[0047] (3) Physiological and biochemical characteristics of bacteria Pseudomonas taiwanensis used in the present invention

[0048] The test results of the physiological and biochemical characteristics of the bacteria Pseudomonas taiwanensi...

Embodiment 2P11

[0055] Experimental analysis of embodiment 2P11 bacterial strain processing water environment mercury

[0056] (1) Mercury speed test kit analysis:

[0057] 1) Test steps:

[0058] ① Preparation of culture medium: prepare 10-fold diluted tryptone soybean broth medium, and then add HgCl to the culture medium 2 , so that Hg 2+ The final concentration is 1mg / L;

[0059] ②Activation of the P11 bacterial classification: get the preserved P11 bacterial classification (when the Abs600 (absorbance value at 600nm) of the activated P11 bacterial liquid is at 0.4-0.5, mix it with 30% glycerol in an equal volume ratio to obtain the preserved P11 bacterial classification. P11 strains are stored below -20°C), inoculated in 10-fold diluted tryptone soybean broth medium according to the volume ratio of 1:100, and cultivated in a shaking shaker at 30°C and 180 rpm for 10-12 hours, that is The Abs600 of the bacterial liquid is between 0.4-0.5, that is, the activated bacterial liquid of P11 ...

Embodiment 3

[0072] Embodiment 3 The inventive method reduces the effect test of total mercury in rice

[0073] 1) Test steps:

[0074] ①Grouping: Divide the mercury-contaminated soil into three groups, group A is the blank control group, group B is the P11 culture solution treatment group, and group C is the microbial organic fertilizer (streptomyces, actinomycetes, Bacillus subtilis , Bacillus megaterium, Bacillus jelly-like, Pseudomonas, etc.) as base fertilizer and then treated with P11 culture medium.

[0075] ② Rice planting: Divide the soil into pots according to groups, and apply 150g / Kg (soil) to group C in the step ① of the microbial organic fertilizer, and then water all the pots to cover the soil, and then cultivate The seedlings in about 13 days are inserted.

[0076] ③P11 culture solution preparation: the activated bacterial solution of P11 described in Example 2 was inoculated in the improved M9 medium (dipotassium hydrogen phosphate 6 g / L, potassium dihydrogen phosphate 3...

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Abstract

The invention discloses a method for reducing total mercury content in rice. The method includes the following steps of preparing a unique strain P11 culture solution, treating rice with P11, applying a compound fertilizer in a tillering stage and a heading stage respectively, performing daily management and the like. By adopting the method for reducing the total mercury content in the rice to plant the rice, the total mercury content in the rice can be obviously reduced; the rice planting production process is relatively simple, only one leaf surface spraying process is added compared with a conventional rice planting method, spraying operation is easy, the workload is small, and the labor cost and the economic cost are low; a used strain comes from a soil environment, the raw materials of a culture medium are simple and easy to obtain, and the culture solution is simple in preparation process, low in energy consumption and low in cost; and the sprayed culture solution is high in environmental safety and small in environmental harm, hardly causes secondary pollution, hardly changes a soil matrix and hardly affects a soil structure.

Description

technical field [0001] The invention relates to the technical field of crop safety production, in particular to a method for reducing total mercury in rice. Background technique [0002] With the development of my country's industrialization, the problem of environmental pollution is becoming more and more serious. In 2014, the Ministry of Environmental Protection and the Ministry of Land and Resources jointly released a survey on the status of soil pollution in the country, pointing out that the total exceeding rate of all pollutants was 16.1%, among which the top The third inorganic pollutants are Cd, Hg, and As in sequence. Among them, mercury damages the central nervous system and has adverse effects on the mouth, mucous membranes and teeth. Mercury can accumulate in the body and is easily absorbed by the skin, respiratory tract and digestive tract. Prolonged exposure to high mercury environments can lead to brain damage and death. [0003] Since mercury has long-range t...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01G7/06C12N1/20C12R1/38
CPCA01G22/22A01G7/06C12N1/20
Inventor 潘继承夏清强汪劲松张新潮万进陈彪彪
Owner HUBEI NORMAL UNIV
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