Method for detecting nine cholesterol oxides in food by gas chromatography-mass spectrometry

Abstract

The invention relates to a method for detecting nine cholesterol oxides in food by gas chromatography-mass spectrometry, which comprises the following steps: S1, adding 19-hydroxycholesterol as an internal standard substance to carry out sample lipid extraction; S2, dissolving the obtained lipid extract, and carrying out saponification extraction to remove moisture so as to obtain a concentrated filtrate; S3, purification and concentration: adding 3mL of n-hexane, shaking and dissolving, adding into activated NH2-SPE, washing the column, discarding waste liquid, collecting filtrate, and blow-drying by nitrogen; S4, derivatization: adding 100 [mu] L of N, O-bis (trimethylsilane) trifluoroacetamide BSTFA for derivatization for 40 min, blow-drying with nitrogen, and dissolving with 1.0 mL of n-hexane to a constant volume; and S5, taking a mixed standard solution of nine cholesterol oxides, detecting the mixed standard solution under gas chromatography conditions and mass spectrometry conditions, and drawing a standard curve. According to the method, a pretreatment mode suitable for complex samples is established, saponification conditions and a solid-phase extraction column are optimized, the accuracy of analysis and detection is improved by adopting an internal standard substance, and the method has the advantages of accuracy, high efficiency and wide applicability.

Description

Technical field [0001] The present invention relates to the field of cholesterol oxide detection techniques, and more particularly to methods of detecting nine cholesterol oxides in foods in gas chromatography - mass spectrometry. Background technique [0002] Cholesterol Oxidation Products, COPS is an oxidative product of cholesterol, generally in meat products, dairy products, egg products and animal fats. During the production process and treatment of food, cholesterol is prone to its own oxidation reaction by light, oxygen and high temperatures, etc., and is derived from cholesterol oxidation products. [0003] Medical studies have shown that foodborne cholesterol oxides will cause certain damage to health. Studies have pointed out that cholesterol oxides and human atherosclerosis, cytotoxic effects, gene mutation, and even carcinogenic relationships. Cholesterol oxides have become a new hot spot for food safety, but the cholesterol oxide in food is still difficult to conduct...

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Problems solved by technology

[0004] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a method for detecting 9 kinds of cholesterol oxides in food by gas chromatography-mass spectrometry, which solves the problem of the limitation in the detection of cholesterol oxides in complex samples in the prior art, and has the advantages of Accurate and efficient, high detection limit, high sensitivity, and good repeatability

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