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Molecular marker for identifying sex of haliotis discus hannai and primer thereof

A technology of wrinkled plate abalone and molecular markers, which is applied in the field of genetic engineering, can solve the problems of quality and stress resistance (decreased survival rate, increased electricity and labor costs for farms, and deterioration of water quality in aquaculture water, etc., and achieves a wide range of applicable materials. , reduce identification errors, and increase economic benefits

Inactive Publication Date: 2021-08-06
JIMEI UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially in the breeding season, the gonads of aquaculture abalone mature and discharge one after another, not only wasting a lot of energy in vain, resulting in a decline in the quality and stress resistance (survival rate) of abalone; it is also easy to cause the water quality of the aquaculture water to deteriorate, increasing the electricity consumption of the farm and labor costs

Method used

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  • Molecular marker for identifying sex of haliotis discus hannai and primer thereof
  • Molecular marker for identifying sex of haliotis discus hannai and primer thereof
  • Molecular marker for identifying sex of haliotis discus hannai and primer thereof

Examples

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Effect test

Embodiment 1

[0026] Example 1: Specific molecular markers of abalone wrinkled

[0027] The applicant of the present invention took the wrinkled plate abalone collected in Jinjiang, Fujian, China, and the Jinjiang Fuda abalone farm as samples, and constructed 11 sets of genome resequencing data of the wrinkled plate abalone, each of male and female, and performed GWAS and The sex dimorphic genotype analysis obtained the genome-wide association analysis results and the sex dimorphic SNP density distribution relationship, and it was further known that the gender-significantly related regions were located on chromosomes 2 and 9. The present invention detects the sexually dimorphic indel of chromosome 2, and finally finds three adjacent SNP sites in the gender-significantly related region of chromosome 2. The red frame marks the three SNPs of the female as heterozygous, and the blue line The 3 SNPs that are boxed to identify males are homozygous (see Figure 1-2 ). figure 1 Middle: F-1 repres...

Embodiment 2

[0029] Example 2: Sex identification of wrinkled pan abalone

[0030] Source of wrinkled plate abalone: ​​54 from Jinjiang population, 60 from Dalian population, and 58 from South Korea population. A total of 172 individuals (83 females and 89 males) from the three populations were used to verify the accuracy of the male-specific molecular marker for gender identification, and the results showed that the sex detection rate was 100%.

[0031] The primer pairs of the present invention are used to carry out PCR amplification on Genomic DNA of Panbao wrinkle, which is determined to be male or female by anatomical examination, and the accuracy and specificity of the method of the present invention are verified by comparing the agarose gel electrophoresis patterns. The specific method steps are as follows:

[0032] 1. Randomly cut 12 parts of the adductor muscle tissue of male and 12 female wrinkled disc abalone adults from three different sources, put them in absolute ethanol, and...

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Abstract

The invention discloses a molecular marker for identifying sex of haliotis discus hannai and a primer thereof. According to the molecular marker, when SNP of 522 bp, 528 bp and 532 bp of SEQ ID NO: 1 are homozygous, the genetic sex is female; and when SNP of 522 bp, 528 bp and 532 bp of SEQ ID NO: 1 are heterozygous, the genetic sex is male. The genetic sex molecular marker has advantages of being applicable to the whole life history, wider in applicable material range and the like.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a molecular marker and a primer for distinguishing the sex of abalone wrinkled. Background technique [0002] Gender is a key link in determining the reproduction of bisexual organisms, and it is also a continuous hotspot in the research of developmental biology and evolutionary biology. Shellfish (molluscs) are closely related to humans. They may be an important protein source for humans, or they may be intermediate hosts for pathogens or invasive organisms that endanger the environment. Shellfish is the second door of the animal kingdom, with a wide range of species, rich and diverse reproductive modes and sex determination methods, which can provide rich materials for the study of the sex determination mechanism and overall evolution of invertebrates. Individual growth and other important economic traits of some shellfish species are closely related to sex; however, the sex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6879C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/6879C12Q1/686C12Q2600/156
Inventor 蔡明夷罗晗娇叶校斌
Owner JIMEI UNIV
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