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Method for identifying Bama minipigs through ISSR molecular marking

A Bamaxiang pig and molecular marker technology, applied in biochemical equipment and methods, microbiological measurement/inspection, etc., can solve problems such as inability to judge unknown samples, achieve obvious differences, reduce identification errors, and ensure varieties Effect

Active Publication Date: 2016-05-04
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no method for identifying Bama pigs, and this primer cannot determine whether an unknown sample is Bama pigs

Method used

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  • Method for identifying Bama minipigs through ISSR molecular marking
  • Method for identifying Bama minipigs through ISSR molecular marking
  • Method for identifying Bama minipigs through ISSR molecular marking

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method of UPGMA cluster analysis using ISSR molecular markers to identify Bamaxiang pigs.

[0026] The method for identifying Bama fragrant pigs comprises the following steps:

[0027] A. Sample DNA extraction;

[0028] B. PCR reaction and product detection;

[0029] C. UPGMA cluster analysis, if the similarity coefficient with the standard product is ≥0.94, the sample can be identified as Bamaxiang pig.

[0030] The nucleotide sequence of the primer used in the PCR reaction is 5'-(ATG) 6 -3'.

Embodiment 2

[0032] Bama pig, Huanjiang pig, Tibetan pig, Bama native pig and Chenghua pig were identified by ISSR molecular markers.

[0033] Weigh 0.5 g of fresh pork samples to be identified from Bama Xiang pig, Huanjiang Xiang pig, Tibetan pig, Bama local pig and Chenghua pig, grind with liquid nitrogen, and extract DNA according to the instructions of the DNA extraction kit. The DNA extraction kit is Tiangen blood / cell / tissue genomic DNA extraction kit TIANampGenomicDNAKit (DP304-02). Use 0.8% agarose gel electrophoresis to detect the integrity of the extracted DNA, and use an ultra-micro protein nucleic acid analyzer to detect the mentioned DNA quality and OD 260nm and OD 280nm Concentration, determination of DNA purity OD 260nm / OD 280nm . Store at -20°C for later use.

[0034] PCR reaction primer, its nucleotide sequence is 5'-(ATG) 6 -3'. The ISSR was amplified using the primers. The PCR reaction system is: template DNA 1.5 μL, primer 1 μL, 2×TaqPCR MasterMix 12.5 μL, ddH ...

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Abstract

The invention provides a method for identifying Bama minipigs through ISSR molecular marking. By specifically combining a specific DNA extraction process, a specific PCR amplification primer and a specific PCR amplification system, an unknown sample can be identified accurately based on distinguishing of Bama minipigs, Huanjiang minipigs, Tibetan pigs, local Bama pigs and Chenghua pigs, so that whether a pig is a Bama minipig is judged. By the adoption of the method, identification is quick, the result is reliable, and the development of the Bama minipig industry can be promoted.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for using ISSR molecular markers to identify Bamaxiang pigs. Background technique [0002] Bama Fragrant Pig originated from Bama Yao Autonomous County, Guangxi. It is a rare and locally advantageous breed of pig. Its meat is tender, sweet and delicious, and has a unique flavor. It can be used to make cured whole pigs, roast suckling pigs, and bacon. Popular with consumers. However, with the increase in demand for it, on the one hand, there are many hybrid breeds in the breeding industry. On the other hand, some illegal traders will use some other breeds of suckling pigs (such as Luchuan suckling pigs) as Bama fragrant suckling pigs for In the production of roasted fragrant pigs and cured fragrant pigs, the products lose the original flavor of authentic fragrant pigs and appear to be shoddy. At present, the relevant identification methods are mainly based on appearance descripti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 孙群耿琦李婉丽金玉兰李松孙宏虎温文婷
Owner SICHUAN UNIV
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