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Anticoagulant antimicroorganism coating and preparation method and use thereof

An antibacterial coating and anticoagulation technology, applied in the field of anticoagulant antibacterial coating and its preparation, can solve the problems of complicated preparation process, difficulty in taking into account antibacterial and anticoagulant properties, low graft density of functional substrates, etc. Simple process, mild and controllable preparation conditions, and improved antibacterial and anticoagulant properties

Pending Publication Date: 2021-08-06
济南金泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention overcomes the drawbacks of the low active group content of the polymer material in the existing surface modification technology, which leads to the low graft density of the functional matrix and the complicated preparation process, and solves the problem that it is difficult to take into account the good antibacterial and anticoagulant properties on the surface of the medical device / material question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) Wash the polished stainless steel test piece (1 cm × 2 cm) in turn with acetone, absolute ethanol, and ultrapure water ultrasound for 5 minutes. The stainless steel was then quickly immersed in a Tris-HCl buffer solution (pH = 8.5) deposited for 1 h, and the addition of polyethylene imine (PEI) (20 mg / ml) was added at room temperature for 30 minutes. After the reaction is completed, 2-4 times was cleaned with deionized water, and then naturally dried in a dry box after cleaning.

[0035] 2) Weigh 0.02 g of carboxymethyl chitosan and 0.02 g of heparin dissolved in 10 mL MES buffer solution (pH = 8.5, 20 mM), then EDC (1- (3-dimethylaminopropyl) -3-ethyl) Carbonedimide) (0.1 m) and NHS (N-hydroxy succinimide) (0.05 m) were added to the above solution. The soaking of the stainless steel treated (1) was soaked in the above mixture for 6 hours. After the removal, the surface was cleaned with PBS, and the sample was washed three times with ultrapure water, and finally was bl...

Embodiment 2

[0038] 1) Clean the Pu film (1.5 cm × 1 cm) in sequentially unhameted ethanol and deionized water, then washed 30 min, then use high purity N. 2 Blow dry. The silicon wafer is cleaned by Piranha lotion, and the volume ratio is 7: 3 in the sample. 2 SO 4 Ho 2 O 2 Mixed liquid, wash it with a large amount of deionized water with high purity N 2 Blow drying.

[0039] Left-vocabula (2 mg / ml) and polyethylene imine (1 mg / ml) were dissolved in 10 ml Tris-HCl buffer (pH = 8.5, 50 mm), and then the solution was oscillated in the constant temperature clock (25 ° C). Reaction for 1 hour. Finally, the cleaned PU film was immersed in the above solution, and the reaction was reacted at room temperature for 8 h. After the reaction was completed, washed with deionized water and placed in the drying tank to dry to constant weight.

[0040] 2) After mixing heparin sodium (2 mg / ml) and CMCs (5 mg / ml) solution 1: 1, EDC (0.1 M) and NHS (0.05 M) were added. Then, the PU film was immersed in t...

Embodiment 3

[0043] As compared with Example 1, the experimental process differs only in the molar ratio of EDC / NHS 1: 1. Other conditions and steps are as in Example 1.

[0044] After dopamine, dopamine / polyethylene imine, the stainless steel test piece after treatment, the water contact angle is 58.25 ± 1.63 °, 37.23 ± 1.34 °, 32.42 ± 0.79 °, respectively, indicating that hydrophilicity is obvious. improve. The thickness of the broken field emission scanning electron microscopy was 18.55 ± 0.22 nm. The diluted coating method showed that after treatment, the stainless steel can kill 80% E. coli and 85% of S. aureus in 20 hours. Raised by qcm measuring heparin graft density to 370 ng / cm 2 . Plasma complex calcification time was increased from 33 min, and the hemolysis rate was reduced by 4% by the original 6%, and the platelet adherence was reduced by 80%, indicating that there is good blood compatibility. The determination is lower toxic to human umbilical vein endothelial cells, and ce...

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Abstract

The invention provides an anticoagulant antimicroorganism coating and a preparation method and use thereof. The anticoagulant antimicroorganism coating based on dopamine-assisted codeposition has a structure as follows: a side close to a base material is taken as an inner side, a dopamine coating and an anticoagulant antimicroorganism composite layer are sequentially arranged from inside to outside and the dopamine coating and the anticoagulant antimicroorganism composite layer are coated on a surface of the base material; wherein the dopamine coating takes dopamine or levodopa and polyethyleneimine as raw materials, and the anticoagulation antimicroorganism composite layer takes an anticoagulation material, an antimicroorganism material, EDC(1-(3-dimethylaminopropyl)-3-ethylcarbodiimide) and NHS(N-hydroxysuccinimide) as raw materials; and hydroxyls and quinonyls of the dopamine coating are covalently bound with aminos and carboxyls of the anticoagulant antimicroorganism composite layer to realize chemical compounding of the two layers.

Description

Technical field [0001] The present invention relates to the field of medical plants / intervention materials, and more particularly to an anticoagulant antibacterial coating and a preparation method thereof. Background technique [0002] The information disclosed in the background is only intended to increase the understanding of the overall background of the present invention, and is not necessarily considered to recognize or imply that the information is intended to be a prior art known to those of ordinary skill in the art. [0003] Medical implant materials, especially blood contact materials, have been widely used in clinical, such as planting / interventional catheter, cardiac valve, hemodialysis film, cardiac pacemaker and cerebrovascular bracket. Although the use of these medical devices greatly improves the medical level, the medical implant material is mostly an inert material, and when it is in contact with the blood, it is easy to cause a thrombus, in addition, bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/10A61L31/08A61L31/14A61L31/16A61L33/10
CPCA61L31/10A61L31/08A61L31/14A61L31/16A61L33/08A61L2420/02A61L2420/08A61L2420/06A61L2300/404A61L2300/42A61L2300/236C08L79/02C08L5/08C08L5/10
Inventor 曹成波杨帆姜元旭王琳张春莲
Owner 济南金泉生物科技有限公司
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