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Application of bifidobacterium lactis MN-Gup in improvement of obesity and characteristic intestinal flora thereof

A technology of bifidobacterium lactis and intestinal flora, applied in the field of microbiology, can solve the problems of affecting product efficacy, unable to specifically regulate obesity-related intestinal flora to restore healthy intestinal flora, single action mechanism, etc., to achieve Reduce the abundance of harmful bacteria, increase the abundance of beneficial bacteria, and inhibit fat accumulation

Active Publication Date: 2021-08-06
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the shortcomings of the existing probiotic products that have a single mechanism of action, cannot specifically regulate obesity-related intestinal flora and restore healthy intestinal flora, thereby affecting the efficacy of the product, thereby Provides a new application of Bifidobacterium lactis MN-Gup in specifically regulating the intestinal flora characteristic of obesity and improving and restoring the intestinal flora, and has a mechanism of action strongly related to the intestinal flora - inhibition Inflammatory factors, reducing blood lipids, and inhibiting adipose tissue degeneration, so as to comprehensively prevent, alleviate and improve obesity or weight gain

Method used

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  • Application of bifidobacterium lactis MN-Gup in improvement of obesity and characteristic intestinal flora thereof
  • Application of bifidobacterium lactis MN-Gup in improvement of obesity and characteristic intestinal flora thereof
  • Application of bifidobacterium lactis MN-Gup in improvement of obesity and characteristic intestinal flora thereof

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Embodiment 1

[0036] The preparation of embodiment 1 bifidobacterium lactis MN-Gup bacterium powder

[0037] (1) Inoculate Bifidobacterium lactis MN-Gup into the MRS medium for activation culture, and culture it statically at 37°C for 16 hours until it reaches the logarithmic phase, and the number of viable bacteria reaches 10 9 More than cfu / mL;

[0038] (2) Bifidobacterium lactis MN-Gup was centrifuged at 10000rpm to collect the thalline, and the protective agent was lactose, skimmed milk powder and other freeze-dried protective agents. Freeze and vacuum dry at ℃ for 24 hours to prepare MN-Gup bacteria powder, the number of bacteria is greater than 4.0×10 11 cfu / g.

Embodiment 2

[0039] The preparation of embodiment 2 Bifidobacterium lactis MN-Gup bacterium powder

[0040] (1) Inoculate Bifidobacterium lactis MN-Gup into MRS medium for activation culture, and culture it statically at 37°C for 18 hours, and cultivate it to the logarithmic phase, and the number of viable bacteria reaches 10 9 More than cfu / mL;

[0041] (2) Bifidobacterium lactis MN-Gup was centrifuged at 12000rpm to collect the thalline, and the protective agent was lactose, skimmed milk powder and other freeze-dried protective agents. Freeze and vacuum dry at ℃ for 24 hours to prepare MN-Gup bacteria powder, the number of bacteria is greater than 3.0×10 11 cfu / g.

Embodiment 3

[0042] The preparation of embodiment 3 Bifidobacterium lactis MN-Gup bacterium powder

[0043] (1) Inoculate Bifidobacterium lactis MN-Gup into MRS medium for activation culture, and culture it statically at 37°C for 16-20h, cultivate to logarithmic phase, and the number of viable bacteria reaches 10 9 More than cfu / mL;

[0044] (2) Bifidobacterium lactis MN-Gup was centrifuged at 11000rpm to collect the thalline, and the protective agent was maltodextrin, skimmed milk powder, etc., and the mass ratio of the added amount of the lyophilized protective agent to the collected thallus was 0.6:1, Freeze and vacuum-dry at -50°C for 21 hours to prepare MN-Gup bacterial powder with a bacterial count greater than 5.0×10 11 cfu / g.

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Abstract

The invention relates to the technical field of microorganisms, relates to application of bifidobacterium lactis MN-Gup to improvement of obesity and characteristic intestinal flora, and discloses bifidobacterium lactis MN-Gup and a microecological preparation thereof, which have the effects of regulating obesity characteristic intestinal flora and improving and restoring health of the intestinal flora, and have the effects that obesity characteristic intestinal flora are regulated; obesity characteristic intestinal flora are improved and regulated in a targeted manner, the abundance of beneficial bacteria related to obesity is improved, the abundance of harmful bacteria related to obesity is reduced, and the health of the intestinal flora is improved and recovered; inflammatory factors IL-1beta, TNF-alpha, IL-6 and INF-gamma are inhibited, and obesity-related inflammatory reactions are improved; triglyceride, cholesterol and / or low-density lipoprotein are reduced, high-density lipoprotein is increased, and the purposes of reducing blood fat and inhibiting fat accumulation are achieved; the adipose tissue degeneration is inhibited; and obesity or weight gain is comprehensively prevented, relieved or improved through the mechanisms.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the use of Bifidobacterium lactis MN-Gup in improving obesity and its characteristic intestinal flora. Background technique [0002] Obesity is a global problem that threatens public health. It not only causes many diseases including type 2 diabetes, coronary heart disease, hypertension, and osteoarthritis, but also imposes a serious economic burden on society. As the "invisible organ" carrying the "second gene" of the human body, the intestinal flora participates in the metabolism of nutrition and energy in the human body, and mediates the occurrence and development of obesity through the intestinal flora-intestine-target organ axis. The gut microbiota diversity and Bacteroides / Firmicutes ratio were decreased in obese patients compared with normal population. In addition, the products and metabolites of intestinal flora can also act on remote organs to affect the pathophysiolo...

Claims

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Application Information

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IPC IPC(8): A61K35/745A61P1/00A61P29/00A61P3/06A61P3/04A23L33/135
CPCA61K35/745A61P1/00A61P29/00A61P3/06A61P3/04A23L33/135A23V2002/00A23V2400/531A23V2200/3204A23V2200/332A23V2200/3262A23V2200/30
Inventor 陈建国康小红孙健栾少萌赵红峰孙二娜韩雨婷
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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