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Resveratrol carbon quantum dots as well as preparation method and application thereof

A technology of resveratrol and carbon quantum dots, which is applied in the field of medicine, can solve problems such as research reports on the inhibitory activity of resveratrol carbon quantum dots to pyocyanin, and achieve clinical application prospects for prevention and treatment, and enhance The effect of inhibitory activity and good clinical application prospect

Inactive Publication Date: 2021-07-20
XUZHOU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no relevant resveratrol carbon quantum dots (CQD Res ) and the research report on improving the inhibitory activity of pyocyanin

Method used

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  • Resveratrol carbon quantum dots as well as preparation method and application thereof
  • Resveratrol carbon quantum dots as well as preparation method and application thereof
  • Resveratrol carbon quantum dots as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] CQD Res Preparation and structural characterization of:

[0029] Drugs: Resveratrol Res was purchased from Sangon Bioengineering (Shanghai) Co., Ltd.

[0030] Resveratrol carbon quantum dots CQD Res Preparation of: CQD by one-step pyrolysis Res preparation. Take 500mg of resveratrol Res and put it into a dry pot, heat and react in a tube furnace at 270°C for 3h. After the furnace temperature dropped to room temperature, the burnt Res was dissolved in ethanol and ultrasonically oscillated for 1 h. Then centrifuge at 15000rpm for 30min to remove insoluble particles. The supernatant was dialyzed in ethanol solution (MWCO=1000Da) for 48h, and then the dialysis bag was dialyzed in ultrapure water for 48h. The mixture in the dialysis bag was transferred into a beaker, pre-cooled at -80°C, and placed in a freeze dryer to lyophilize into powder. Experimental results: CQD Res It is a black powder with high solubility in ethanol and DMSO.

[0031] CQD Res Structural cha...

Embodiment 2

[0040] CQD Res To enhance the inhibitory activity of pyocyanin: pick a single colony of Pseudomonas aeruginosa PAO1 into LB medium, and culture it at 37°C and 180rpm for 17h. Take 5 μL of the bacterial solution and add it to 5 mL of PDB medium (20 g of peptone, 1.4 g of magnesium chloride, 10 g of potassium sulfate, 10 g of glycerol, 1000 mL of water, pH 7.3), and add CQD at a final concentration of 6.25-100 μg / mL Res , with DMSO as the negative control and Res as the positive control, cultured at 37°C and 180rpm for 17h. Take the culture solution and centrifuge at 12000rpm for 10min, take 200μL of the supernatant and measure the OD in a microplate reader 695 .

[0041] Test results: by figure 2 It can be seen that CQD Res After treatment, the inhibitory activity of pyocyanin was significantly enhanced, CQD Res The effect of inhibiting the synthesis of pyocyanin at the subinhibitory concentration is equivalent to 4 times of the same concentration of Res.

Embodiment 3

[0043] Cell membrane phospholipid composition experiment: Inoculate a single colony of Pseudomonas aeruginosa PAO1 into M9 medium (Na 2 HPO 4 .7H 2 O12.8g, KH 2 PO 4 3g, NaCl 0.5g, NH 4 Cl 1g, MgSO 4 .7H 2 O 0.5g, FeSO 4 10mg, MnCl 2 10mg, glucose 4g, water 1000mL, pH 7.0), cultured at 37°C, 180rpm for 17h. Take 30 μL of the bacterial solution and add it to 30 mL of M9 medium, add 100 μg / mL of CQD Res . With DMSO as negative control and 100 μg / mL Res as positive control, culture at 37° C. and 180 rpm for 17 hours. Set up 9 repetitions for each set. Take the cultured bacterial solution and centrifuge it at 12000rpm for 10min, collect the bacterial cells, and wash 3 times with PBS. Add 3.8 mL of pre-cooled methanol / water (1 / 0.9, v / v), ultrasonically break completely, add 4 mL of chloroform, vortex fully, and centrifuge at 12000 rpm for 10 min. Take the upper methanol / water phase, blow off the methanol with a nitrogen blower, freeze in a -80°C refrigerator for 24 ...

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Abstract

The invention discloses resveratrol carbon quantum dots as well as a preparation method and application thereof. The resveratrol carbon quantum dots are prepared by a resveratrol Res pyrolysis method, can enhance the inhibitory activity of pyocyanin, change the phospholipid composition of a cell membrane and inhibit the expression of fatty acid synthesis related genes, and has an application prospect in preparation of drugs for resisting pseudomonas aeruginosa infection.

Description

technical field [0001] The invention belongs to medical technology, and in particular relates to resveratrol carbon quantum dots and their preparation and application. Background technique [0002] The misuse of antibiotics has exacerbated the drug resistance of Pseudomonas aeruginosa. Transforming drug delivery strategies, discovering new targets, and developing drugs with novel antivirulence mechanisms are important solutions to deal with drug resistance of Pseudomonas aeruginosa. Traditional antibacterial drugs work by inhibiting bacterial growth or sterilizing bacteria, and are prone to drug resistance. However, non-classical antibacterial drugs targeting bacterial virulence factors do not aim at killing bacteria. By weakening the pathogenicity of bacteria and eliminating bacteria with the help of the body's own immunity, it is not easy to generate selection pressure for drug resistance, and has the advantage of reducing or delaying the emergence of drug resistance. . ...

Claims

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Application Information

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IPC IPC(8): A61K33/44A61K31/05A61P31/04C01B32/15B82Y5/00
CPCA61K33/44A61K31/05A61P31/04C01B32/15B82Y5/00A61K2300/00
Inventor 周金伟王颖唐皓哲姬鹏程王昌耀杨雨洁
Owner XUZHOU UNIV OF TECH
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