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Delivery system based on nano-liposome, preparation method and application thereof

A nano-liposome and delivery system technology, applied in the field of medicine, can solve problems such as unstable solubility of ozone, achieve the effect of increasing stability and improving hypoxic environment

Active Publication Date: 2021-07-13
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ozone is unstable and has low solubility, so an efficient and stable ozone delivery system is needed, which can deliver ozone to the tumor microenvironment at a fixed point

Method used

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  • Delivery system based on nano-liposome, preparation method and application thereof
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  • Delivery system based on nano-liposome, preparation method and application thereof

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0034] Such as figure 1 As shown, the nanoliposome-based ozone delivery system was prepared according to the following steps,

[0035] Step 1: Add 400 μL of perfluorocarbon and 100 μL of Tween-80 to the solvent, the solvent is 600 μL of acetic acid with a concentration of 0.6 mol / L; vortex for 15 minutes, then sonicate at 35W for 10 seconds, pause for 5 seconds, and cycle in total 10 times, a perfluorocarbon (perfluorodecalin, PFD) emulsion can be obtained. (The PFD emulsion group in the attached figure is the emulsion obtained by this step)

[0036] Step 2: 300 mg lecithin and 100 mg cholesterol were dissolved in a solvent, and the solvent was 10 mL of chloroform. Under the condition of a water bath at 42°C, the solution was rotary evaporated under reduced pressure, and then dried in a round-bottomed flask to form a lipid film.

[0037] Step 3...

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Abstract

The invention discloses a delivery system based on nano-liposome, a preparation method and an application thereof. The nano-liposome is of a granular structure composed of a shell layer and emulsion in the shell layer. The shell layer is composed of lecithin and cholesterol, and the emulsion is composed of perfluorocarbon and Tween-80; wherein the mass ratio of lecithin to cholesterol is (1-4): (0.5-1); the volume ratio of the perfluorinated carbon to the tween-80 is (1-6): (0.5-1.5); and the average particle size of the liposome is 100 nm to 300 nm. The preparation method comprises the following steps: firstly, perfluorocarbon is prepared into an emulsion, and then the emulsion is wrapped in a liposome; when the liposome is combined with ozone, the ozone is coated in the liposome, so that the stability of the ozone and the concentration of the carried ozone are improved; the nano-liposome loaded ozone is specifically delivered into a tumor microenvironment, under the action of rays, the ozone can be decomposed into hydroxyl radicals with higher concentration compared with oxygen, the effect of directly killing tumor cells is achieved, meanwhile, the ozone can also be decomposed to generate oxygen, and the killing sensitivity of radioactive rays to the tumor cells is enhanced by improving the hypoxic tumor microenvironment.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a nanoliposome-based delivery system, preparation method and application. [0002] technical background [0003] Radiation therapy is one of the main options for local treatment of tumors. The principle is that when a large amount of radiation enters the organism, it will destroy the DNA, RNA, protein and other biological functional macromolecules of the cell. In particular, it causes double-strand breaks in important genetic material DNA (deoxyribose nucleic acid), thereby causing irreparable damage to cells in a state of vigorous division (mostly tumor cells). The killing effect of radiation on cells is directly proportional to the growth rate of cells and inversely proportional to the degree of differentiation of cells. However, tumor cells grow faster than normal cells and have a lower degree of differentiation. The killing effect of radiation on them is much greater than ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K33/00A61K47/06A61P35/00
CPCA61K9/127A61K33/00A61K47/06A61P35/00
Inventor 石虎兵李燕雏郑丹
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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